| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,854,401 | 0 | A | C | 40.0% | 77.6 / 23.5 | 60 | K88N (AAA→AAC) | yeaO | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (21/15); new base C (0/24); total (21/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.21e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACG > REL606/1854326‑1854475 | cTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGGGGGGAGCG‑CCacaaaa > 1:3046670/1‑76 (MQ=255)cTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCaaaaaa < 2:1361630/76‑1 (MQ=255) gttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAAc < 2:1743238/75‑1 (MQ=255) ttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGGGGGGAACG‑CCacaaaaaa > 2:1986975/1‑74 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAg < 1:3885678/76‑1 (MQ=255) cgACCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCg < 2:922673/76‑1 (MQ=255) tcAATATCTTGAAGAACTGGCGCAACCCGAGCAAGAAGGAAGGCGG‑CGGGCGGACACCG‑CAAAAAAACAGCCGCTg < 2:2578884/75‑1 (MQ=255) tatCTTGCAGAACTGGCGCAACACGAGAAAGAAGGAAGGCGG‑CTGGCGGCCATCG‑CCAAAAACCAGCCGCTGAccc < 2:3255458/76‑1 (MQ=255) atCTTGTAGAACGGGCGCAACACGAGAAAGAAGGAAAGCGG‑CTGGCGGAAACCG‑CCAAAAACCAGCCGCTGACCCt < 2:3486185/76‑1 (MQ=255) tCTTGCAAAACTGGCGAAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTg < 1:1807412/76‑1 (MQ=255) gCAGAACGGGCGCAACCGGAAAAAGAAGAAAGGCGG‑CTGGCGAACATCC‑CAAAAAACCAGCCGCTGACCCTGctct < 2:4627933/76‑1 (MQ=255) gCAGAACGGGCGCAACACGAGAAAGAAGGAAAGCGG‑CGGGCGGACATCG‑CCAAAAACCAGCCGCTGACCCTGctct < 2:1505596/76‑1 (MQ=255) cAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTa < 1:523320/76‑1 (MQ=255) cAGAACTGGCGCAACACGAGAAAGAAGGAAAGCGG‑CGGGCGGACATCG‑CCAAAAACCAGCCGCTGACCCTGCTCTa < 2:3843336/76‑1 (MQ=255) cAGAACTGGCGAAACACGAGAAAGAGGGAAGGCGG‑CGGGCGGACATCC‑CAAAAAACCAGCCGCTGACCCTGCTCTa < 2:2833392/76‑1 (MQ=255) aataaCGGGCGCAACACGAGCAAGAGGAAAAGCGG‑CTGGCGGAAATCG‑CAAAAAAACAGCCGCTGACCCTGCTCTa < 2:1080690/73‑1 (MQ=255) aGAACTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAACCAGCCGCTGACCCTGCTCTAc < 1:2552163/76‑1 (MQ=255) gAACTGGCGCAACCCGACCAAGAAGGAAAGCGG‑CTGGCGAACATCG‑CCAAAAACCAGCCGCTGACCCTGCTCTACg < 2:2111396/76‑1 (MQ=255) aaCTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACgc < 2:4701250/76‑1 (MQ=255) aaCGGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAACCAGCCGCTGACCCTGCTCTACgc < 2:2905180/76‑1 (MQ=255) gggCGAACCACGAGAAAGAAGAAAAGCGG‑CTGGGGGACATCC‑CAAAAAACCAGCCGCTGACCCTGCTCTACgcagc < 2:411487/75‑1 (MQ=255) cgcACCACGAGAAAGAAGGAAAGGGG‑CTGGCGGACATCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaa < 1:4046540/76‑1 (MQ=255) cgcAACACGGAAAAGAA‑GATAGCGGTGTGCCGGACTGCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaa < 2:1568639/76‑1 (MQ=255) cgcAACACGAGCAGGAAGGAAAGCGG‑CGGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaa < 1:131794/76‑1 (MQ=255) cAACACGAGCAAGAAGGAAAGCGG‑CTGGGGGGCATCG‑CCCAAAAAAAACCGCCCACCCCCCCCTCCGCAGCaaaaa > 1:3950943/1‑76 (MQ=255) cAACACGAGCAAGAAGGAAAGCGG‑CTGGGGGGAATCGACCAAAAAAAAACGGCCG‑CCCCGCTCCACGCAGCaaaaa > 2:4904270/1‑76 (MQ=255) cAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaaaa < 1:4716045/76‑1 (MQ=255) cAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaaaa < 1:3475999/76‑1 (MQ=255) acccGAGCAAGAAGGAAAGCGG‑CTGGCGGCCATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAac < 1:4639516/73‑1 (MQ=255) gaaGCAAAAAGAAAGACGG‑CTGGCGGACACCC‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacc < 2:3967225/74‑1 (MQ=255) cGAGCAAGAAGGAAGGCGG‑CTGGCGGACATCG‑CAAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacc < 1:2018079/76‑1 (MQ=255) cGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacc < 1:2695950/76‑1 (MQ=255) gagaAAGAAGGAAAGCGG‑CGGGCGGACATCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacca < 2:1197194/76‑1 (MQ=255) gCAAGAAGGAAAGGGG‑CTGGCGGACACCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACg < 1:2795091/76‑1 (MQ=255) gaagGAAAGCGG‑CTGGCGGACATCG‑CCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGa < 1:351915/76‑1 (MQ=255) gaagGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGa < 1:4998108/76‑1 (MQ=255) gaagGAAAGCGG‑CTGGCGGACATCG‑CAAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGa < 1:1755319/76‑1 (MQ=255) aagGAAAGCGG‑CTGGCGGACATCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGaa < 1:4665477/76‑1 (MQ=255) ggAAGGCGG‑CTGGCGGACATCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 2:1288200/76‑1 (MQ=255) gaaaaGCGG‑CTGGGGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAcc < 2:215413/74‑1 (MQ=255) gAAGGCGC‑CGGGCGGACATCG‑CCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCa < 1:377469/76‑1 (MQ=255) gAAAGCGG‑CGGCCGGACATCG‑CCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCa < 2:2895650/76‑1 (MQ=255) aGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgc < 1:97648/76‑1 (MQ=255) gg‑CTGGCGGGCATCG‑CCAAAAAAAAGCCGCTGACCCCGCTCTTCGCAGCCAAAAAAACCACGCAGAACCATGCGCt > 2:971449/1‑76 (MQ=255) g‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg > 2:1218452/1‑76 (MQ=255) g‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg > 2:1879421/1‑76 (MQ=255) g‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAACATGCGCTg > 2:1383146/1‑76 (MQ=255) g‑CTGGCGGACATCC‑CCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg < 1:4552072/76‑1 (MQ=255) g‑CTGGCGGACACCG‑CAAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg < 2:749801/76‑1 (MQ=255) gcgggCGGACATCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg < 2:34621/73‑1 (MQ=255) gcgggCGGACATCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg < 2:4286291/73‑1 (MQ=255) gcgggCGAACACCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg < 2:2619858/73‑1 (MQ=255) cTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg > 2:495232/1‑76 (MQ=255) cTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg > 2:1294201/1‑76 (MQ=255) cTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg > 2:1292791/1‑76 (MQ=255) cTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg > 1:4968139/1‑76 (MQ=255) tGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGTGCTGGt > 1:3185966/1‑76 (MQ=255) tGGCGGACACCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt < 2:4241732/76‑1 (MQ=255) gggCGGACACCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt < 2:141373/75‑1 (MQ=255) ggCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTg > 2:2968479/1‑76 (MQ=255) gCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGc > 1:4872279/1‑76 (MQ=255) gggACACCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt < 2:3191875/75‑1 (MQ=255) cGGACATCG‑CCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt < 1:863648/76‑1 (MQ=255) cGGACATCG‑CCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt < 1:3762383/76‑1 (MQ=255) cGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 2:1024504/1‑76 (MQ=255) cGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 2:2905020/1‑76 (MQ=255) gACACCG‑CAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTgg < 1:155352/76‑1 (MQ=255) tcATCG‑CAAAAACCCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGc < 2:2628727/75‑1 (MQ=255) aaaTCG‑CCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGc < 2:2656160/74‑1 (MQ=255) aCATCG‑CCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGc < 2:1387937/76‑1 (MQ=255) accG‑CAAAAACCCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg < 2:380010/74‑1 (MQ=255) aTCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg > 2:685712/1‑76 (MQ=255) aTCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg > 2:1326471/1‑76 (MQ=255) tCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGa > 2:3181079/1‑76 (MQ=255) tCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGa > 2:4670302/1‑76 (MQ=255) tCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGa > 2:167099/1‑76 (MQ=255) ccAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg < 2:4828988/76‑1 (MQ=255) ccAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg > 1:3975901/1‑76 (MQ=255) ccAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg > 2:1664465/1‑76 (MQ=255) caaaaaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg < 2:227976/74‑1 (MQ=255) cAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTgg > 1:1194133/1‑76 (MQ=255) caaacCAGCCGCGAACCCTGCTCTACGCAGCAAAAACCACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 2:560506/75‑1 (MQ=255) aaacaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 1:227735/76‑1 (MQ=255) aaaacCAGCCGCTGATCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 2:1922998/76‑1 (MQ=255) aaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 2:1447327/76‑1 (MQ=255) aaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 2:1745754/76‑1 (MQ=255) aaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 1:1790209/76‑1 (MQ=255) aaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt < 2:560505/76‑1 (MQ=255) aaaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCt > 1:1220412/1‑76 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 2:4972543/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 2:2692766/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 2:2290011/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 1:3870420/76‑1 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 1:2369462/76‑1 (MQ=255) aaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa > 2:3426992/1‑76 (MQ=255) aaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa > 2:1528587/1‑76 (MQ=255) aaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTaag > 2:1739447/1‑74 (MQ=255) | CTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGG‑CTGGCGGACATCG‑CCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACG > REL606/1854326‑1854475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |