| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,378,885 | 0 | T | G | 35.2% | 239.1 / 31.4 | 125 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
| Reads supporting (aligned to +/- strand): ref base T (20/61); new base G (44/0); total (64/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-18 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 150 total aligned reads displayed.
TGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378812‑3378960 | tGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGCTGTTGCGCATCGGCAGGgggt > 2:431946/1‑76 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGtt < 2:2158144/76‑1 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGtt > 2:1805058/1‑76 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGtt < 2:4295455/76‑1 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTa < 1:1072543/76‑1 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTa < 1:1712345/76‑1 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTa < 1:4647546/76‑1 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGttt > 1:610373/1‑75 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTAc < 1:2524747/76‑1 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTAc > 2:1167093/1‑76 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTAc > 1:2677160/1‑76 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTgg > 1:897165/1‑76 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGGTACCTggg > 2:1497534/1‑75 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTggg > 1:6059/1‑75 (MQ=255) tACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGttt < 2:2647605/76‑1 (MQ=255) tACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTATCTGGttt > 1:3078957/1‑76 (MQ=255) tACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGgtt > 1:3457968/1‑76 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTg > 1:4005778/1‑76 (MQ=255) ccTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTGCCTGGGTTGa > 2:239986/1‑76 (MQ=255) ccTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGa < 2:1780500/76‑1 (MQ=255) ggTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTATCCGGGTTGTTTg > 2:1087320/1‑76 (MQ=255) gTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgc < 2:3527461/76‑1 (MQ=255) gTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTCGCCGGTTTGATTgc > 2:3571157/1‑76 (MQ=255) tttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTACCGGGTTGATTgca > 1:1847120/1‑76 (MQ=255) ttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcag > 2:3802646/1‑76 (MQ=255) ttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcag < 1:1198570/76‑1 (MQ=255) ttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTTATTgcag > 2:1915606/1‑76 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTgcagc > 1:3039603/1‑76 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTgcagc > 2:638014/1‑76 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTTCCTGGTTTGATTgcagca < 2:2331490/76‑1 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTtcagca > 2:3646781/1‑76 (MQ=255) aTTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCAt < 1:3170944/76‑1 (MQ=255) aTTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCAt > 1:783705/1‑76 (MQ=255) aTTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGTTTGATTGCAGCAt > 1:4258279/1‑76 (MQ=255) tttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATc < 2:796119/76‑1 (MQ=255) ttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCa < 1:2830318/76‑1 (MQ=255) ttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCa < 2:4050753/76‑1 (MQ=255) ttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCa > 1:803812/1‑76 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGGAGCATCAAc > 1:1432512/1‑76 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAAc > 1:2009695/1‑76 (MQ=255) tACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACg < 1:558588/76‑1 (MQ=255) cGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGcc < 1:1458191/76‑1 (MQ=255) cGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGcc < 2:4596031/76‑1 (MQ=255) gTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCAACGCCa > 2:2276208/1‑76 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGGAGCATCAACGCCaa > 1:2254247/1‑75 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGcccg > 1:2939715/1‑74 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGTTTTATTGCAGCATCAACGCCAGAc > 2:3091500/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGTTTGATTGCAGCATCAACGCCAGaa > 1:1469092/1‑75 (MQ=255) tGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACt < 2:2194735/76‑1 (MQ=255) tGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACt < 1:707397/76‑1 (MQ=255) gAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTg < 1:2521870/76‑1 (MQ=255) aaCAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCAACGCCAGACTGa > 2:1270207/1‑76 (MQ=255) cAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTTCCTGGTTTGATTGCAGCATCAACGCCAGACTGAAt > 2:3797446/1‑76 (MQ=255) cAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTAACTGGTTTGATTGCAGCATCAACGCCAGACTGAAt > 2:4672873/1‑76 (MQ=255) aGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATc > 1:4947208/1‑76 (MQ=255) gTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCt < 2:3250183/76‑1 (MQ=255) gTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCt < 1:4675020/76‑1 (MQ=255) tAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCCGCATCAAACCCAGACTGAAACTg > 2:1188892/1‑76 (MQ=255) tAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTg < 1:4026854/76‑1 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCCGACTGAATCTgg > 2:2395512/1‑76 (MQ=255) gAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGt < 2:584844/76‑1 (MQ=255) gAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCCCGGTTTGATTGCCGCATCAACCCCCGACCGAATCTGGt > 2:2706201/1‑76 (MQ=255) aaccaaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTc < 1:112911/76‑1 (MQ=255) aaccaaccGGGATGTTGCGCATCGGCAGGGGGGTCCCTGGTTTGATTGCAGCATCAACCCCAGACTGTATCTGGTc > 2:3780003/1‑76 (MQ=255) accaaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCg < 2:4941666/76‑1 (MQ=255) accaaccGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCg > 2:333244/1‑76 (MQ=255) aaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCa < 1:3925935/76‑1 (MQ=255) aaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCa < 1:4354333/76‑1 (MQ=255) aaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCa < 1:2805901/76‑1 (MQ=255) gggATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCtt < 2:3572309/76‑1 (MQ=255) gggATGTTGCGCATCGGCAGGGGGTTACCTGGTTTTATTGCAGCATCAAAGCCAGACTGAATCTGGTCGCCAGGtt > 2:4033866/1‑76 (MQ=255) ggATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCttt < 2:1261502/76‑1 (MQ=255) gATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTc < 1:3752807/76‑1 (MQ=255) gATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTc < 1:4747261/76‑1 (MQ=255) aTGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCa < 2:3078957/76‑1 (MQ=255) aTGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCa < 2:3335116/76‑1 (MQ=255) aTGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCa < 2:123956/76‑1 (MQ=255) tGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAg < 2:3298970/76‑1 (MQ=255) tGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAg > 2:2067347/1‑76 (MQ=255) gTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATAAACGCCAGACTGAATCTGGTCGCCAGCTTTCAgg > 1:4707295/1‑76 (MQ=255) ttGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATAAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGc > 2:2168579/1‑76 (MQ=255) cgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCtt < 2:492884/76‑1 (MQ=255) gcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCttt < 2:4548564/76‑1 (MQ=255) gcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCttt < 2:4523855/76‑1 (MQ=255) cATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTa < 2:2617314/76‑1 (MQ=255) cATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTa < 1:4872240/76‑1 (MQ=255) cATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTa < 2:3755959/76‑1 (MQ=255) cATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTa < 2:3982337/76‑1 (MQ=255) cATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAAACTGGTCGCCCGCTTTTAGGCCTTTa > 2:1705013/1‑76 (MQ=255) aTCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAg < 1:4733954/76‑1 (MQ=255) aTCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAg < 1:715131/76‑1 (MQ=255) aTCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAg < 2:2733674/76‑1 (MQ=255) cGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAggg > 2:3195709/1‑76 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 2:1482574/1‑76 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 2:4455576/1‑76 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 2:3474747/1‑76 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 2:3050106/1‑76 (MQ=255) aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 2:4300240/1‑76 (MQ=255) aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 2:529459/1‑76 (MQ=255) ggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAgg < 1:905302/76‑1 (MQ=255) | TGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378812‑3378960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |