| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,774,914 | 0 | C | G | 44.0% | 127.6 / 116.1 | 116 | A20P (GCC→CCC) | yicJ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (1/64); new base G (51/0); total (52/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-32 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 145 total aligned reads displayed.
CGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCTTGATGCT > REL606/3774839‑3774989 | cGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGtggctggc < 1:3687832/76‑1 (MQ=255) ggCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggcg < 2:1790912/76‑1 (MQ=255) ggCAGGAATGCCAAAAATATCGGTATAAAAGAACATC‑‑ATATAACATTACGTTATCGAAAATAATGTGGCTggcggc < 1:836907/76‑1 (MQ=255) gCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggggg > 1:3746753/1‑76 (MQ=255) cAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTgggggg > 1:2368932/1‑75 (MQ=255) aGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGgcggcg < 2:3049160/76‑1 (MQ=255) ggAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGt < 2:650353/76‑1 (MQ=255) gCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGactaac > 2:1128275/1‑73 (MQ=255) gCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTccc < 1:4556512/76‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTcccc > 1:4242312/1‑75 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTcccc > 1:4814573/1‑75 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCa < 1:3126147/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 1:2203885/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 1:4077791/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 1:4930403/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 1:177797/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 2:1909686/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 1:681942/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 2:3177065/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 1:944657/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 2:3202997/76‑1 (MQ=255) aaaaaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATg < 1:4428686/76‑1 (MQ=255) aaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCCTGccc > 2:4160015/1‑75 (MQ=255) aaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCCTGCCa > 1:4444498/1‑76 (MQ=255) aaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCa < 1:246953/76‑1 (MQ=255) atatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCGCCCACGCCaa > 2:1205772/1‑75 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCACTCCAT‑a > 2:4514365/1‑76 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGCGGCTCCCCTGCCCT‑aa > 1:993958/1‑76 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑aa < 2:3090037/76‑1 (MQ=255) cGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCCCCCCTGCCAT‑aaaa > 2:3634706/1‑74 (MQ=255) cGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AAcc < 2:3388978/76‑1 (MQ=255) ggTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCAC‑AACCa > 2:3123410/1‑76 (MQ=255) ggTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCa < 1:2680037/76‑1 (MQ=255) gTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCaa < 1:3628492/76‑1 (MQ=255) gTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCaa < 1:1726720/76‑1 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCATGCCAT‑AACCCAt > 1:113040/1‑76 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCACGGCAT‑AACCaaa > 1:2368022/1‑75 (MQ=255) taAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCCT‑AAACAAatt > 1:3856761/1‑76 (MQ=255) aaaaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑CACCAAattt > 2:2227936/1‑76 (MQ=255) aaaaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCAT‑AACCAAattt > 1:2797078/1‑76 (MQ=255) aaaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCAC‑TAACAAttttt > 2:4133574/1‑75 (MQ=255) aatcatcaaATACAACATAACGTTACCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTctc‑t < 1:4114435/73‑1 (MQ=255) aaCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AACCCAATTTTTC‑tt > 1:4653937/1‑76 (MQ=255) aaCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTATC‑tt < 1:3236288/76‑1 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑ttt < 1:2159947/76‑1 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑ttt < 1:2735632/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCCCGCCCT‑AACCAAATTTTTC‑tttt > 1:3310038/1‑75 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTa < 2:4374500/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTa < 2:3269262/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTa < 2:3643592/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTa < 1:3713863/76‑1 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTaa < 2:4987452/76‑1 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCAT‑AACCAAATTTCTC‑TTTTACg > 1:712914/1‑76 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACg < 1:1461853/76‑1 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCGC‑AAACAAATTTTTC‑TTTTAcag > 1:4063170/1‑74 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCATGCCCTAAACAAATTTTTTT‑TTTAACg > 1:3090420/1‑76 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACgg < 1:333247/76‑1 (MQ=255) tatataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGTCTCCCATCCCCT‑AAACCACTTTTTC‑TTTTACAG‑a > 2:3734242/1‑76 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGGCCT‑TACCAAATTTCTC‑TTTTACCG‑aa > 2:3615527/1‑75 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGCGGCTCCCCTGCCCT‑AACCAATTTTTTC‑TTTTAAGG‑ac > 1:2550532/1‑76 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑ac < 2:449719/76‑1 (MQ=255) ataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTCCCAT‑AACCAAATTTTTC‑TTTTACAG‑ac‑aa > 2:815037/1‑76 (MQ=255) aCATTACGTTATCGAAAATAATGTGGCTGGGGGGATCTCCCCTGCCAC‑AAACAATTTTCTC‑TTTTACTC‑AG‑Aacac > 2:4975076/1‑76 (MQ=255) cATTACGTTATCGAAAATAATGTGGCTGGGGGGGTCTCCCATGCCAT‑AAACAATTTTCTC‑TTTTACGG‑ac‑aacaat > 1:2321375/1‑74 (MQ=255) cATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACt < 2:1851453/76‑1 (MQ=255) ttACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCCT‑AAACAATTTTCTT‑TTTTACGG‑AC‑AAAACTTc > 1:2481129/1‑76 (MQ=255) ttcGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCa < 2:787949/74‑1 (MQ=255) tACGTTATCGAAAATAATGTGGCTGGGGGGGGTGCCCCTGCCAT‑AACCAATTTTTTCTTTTAACGGAac‑aacatca > 1:136360/1‑73 (MQ=255) aCGTTATCGAAAATAATGTGGCTGGGGGGGTCGCCCCCGCCCT‑AACCAAATTTTTC‑TTTTACAG‑AC‑AACACATccc > 1:3186922/1‑74 (MQ=255) cGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTCCCCT‑AACCAAATTTTTCTTTTAACGG‑‑C‑AACACCTCAcc > 2:2719774/1‑75 (MQ=255) gTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCG‑AACCAAATTTTTC‑TTTAAAAT‑AC‑AAAACATCAccc > 1:4461461/1‑74 (MQ=255) ttATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCActct < 2:1344322/76‑1 (MQ=255) ttATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCActct < 2:3857546/76‑1 (MQ=255) tATCGAAAATAATGTGGCTGGGGGGGGGGCCCCACCCAT‑CAACAACTTTCTC‑TTTTACTG‑AC‑AACACTTCAcactc > 2:444514/1‑75 (MQ=255) tATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AACCCATTTTTTT‑TTTTATGG‑AA‑AAAACCTCACTCtt > 2:4719277/1‑76 (MQ=255) tATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCAT‑AAACAATTTTCTT‑TTTTACAG‑ACAAACCCTTCAcctt > 2:1117136/1‑73 (MQ=255) aTCGAAAATAATGTGGCTGGGGGGGTCTCCCATCCCCT‑AACCAACTTTCTCTTTTAACGG‑‑C‑AAAACCTCACCCTTc > 1:2310723/1‑76 (MQ=255) cGAAAATAATGTGGCTGGCGGGGGCTCCCCGGCCCT‑AACCAACTTTTTC‑TTTATCTG‑CC‑AACACATCACCCCTCAt > 2:2613069/1‑76 (MQ=255) cGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCAt < 1:1818902/76‑1 (MQ=255) cGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCAt < 1:1097825/76‑1 (MQ=255) aaaaTAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AACCAAATTTCTC‑TTTTACGG‑AC‑AAAACATTACTCTTCATCt > 2:3374669/1‑76 (MQ=255) aaaaTAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCt < 2:465562/76‑1 (MQ=255) aaaTAATGTGGCTGGGGGGGGCTCCCCTCCCCT‑AAACAATTTTTTC‑TTTTAAGG‑AC‑AACACTTTACTCTTCATCtc > 2:4346455/1‑75 (MQ=255) aataatGTGGCTGGGGGGGGCTCCCCCGCCCT‑AAACAATTTTTTT‑TTTTACCG‑AC‑AACAATTCACTCTTTATCttc > 2:665466/1‑74 (MQ=255) aataatGTGGCTGGGGGCGGCTCCCCCGCCCT‑AACCAATTTTTTC‑TTTTAACG‑AC‑CACAACTCACTCCTCATCttc > 2:4194507/1‑74 (MQ=255) ataatGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCt < 1:2627462/76‑1 (MQ=255) taatGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCtt < 2:1901054/76‑1 (MQ=255) taatGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCtt < 2:3696726/76‑1 (MQ=255) tgtgGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTc < 2:1817970/76‑1 (MQ=255) ggctggcGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCtt < 1:1115232/76‑1 (MQ=255) ggctggcGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCtt < 2:1586521/76‑1 (MQ=255) gctggcGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCTTg < 1:940605/76‑1 (MQ=255) gctggcGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACCCTTCATCTGCTTTTCCTTg < 1:1925444/76‑1 (MQ=255) ctggcGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCTTGa > 1:362244/1‑76 (MQ=255) tggcGGCGTCTCCCCTGCCAT‑ACCCAATTTTCTC‑TTTAACGG‑CC‑AAAACTTCACTCTTCATGTGTTTTTCCTTGAt > 2:2136854/1‑76 (MQ=255) tggcGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCTTGAt > 2:3227923/1‑76 (MQ=255) ggcggcGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCTTGATg > 2:1231194/1‑76 (MQ=255) ggcggcGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCTTGATg > 1:968102/1‑76 (MQ=255) cggcgTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCTTGATGCt < 1:4265996/76‑1 (MQ=255) cggcgTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCTTGATGCt < 1:1482652/76‑1 (MQ=255) | CGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCTC‑TTTAACGG‑AC‑AACACTTCACTCTTCATCTGCTTTTCCTTGATGCT > REL606/3774839‑3774989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |