| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,320,225 | 0 | T | G | 34.3% | 152.2 / 54.0 | 99 | T40P (ACC→CCC) | melR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (9/56); new base G (34/0); total (43/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-18 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 129 total aligned reads displayed.
TTTCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGG > REL606/4320150‑4320299 | tttCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCtggt < 1:57711/76‑1 (MQ=255) cATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGGCCTGACCATGCCAGTGGCTGGTggg < 1:2742016/76‑1 (MQ=255) cATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTggg > 1:4424493/1‑76 (MQ=255) aTTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTggggggg > 2:3809094/1‑75 (MQ=255) aTTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTggggggg > 2:804119/1‑75 (MQ=255) ttgttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTgggggggg > 2:4554353/1‑74 (MQ=255) ttgttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGcg > 2:362918/1‑75 (MQ=255) ttgttgATCAGGTATTCCACATCGCCAACGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGcg > 1:2603394/1‑75 (MQ=255) tgttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTggggggggc > 1:831726/1‑73 (MQ=255) tgttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCAt < 2:3891574/76‑1 (MQ=255) gttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATg < 2:2899038/76‑1 (MQ=255) gttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATg < 2:199542/76‑1 (MQ=255) gttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATg < 2:1059785/76‑1 (MQ=255) tgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGat < 2:4267940/76‑1 (MQ=255) tgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGat > 2:611213/1‑76 (MQ=255) gATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGata < 1:2632927/76‑1 (MQ=255) gATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGata < 2:3362859/76‑1 (MQ=255) gATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGata < 2:2896893/76‑1 (MQ=255) aTCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGAAGata‑t > 2:380193/1‑76 (MQ=255) tCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑Tg < 1:439503/76‑1 (MQ=255) cAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGCGTGATA‑TGc > 1:3737430/1‑76 (MQ=255) aGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGATA‑TGCg > 1:1020392/1‑76 (MQ=255) ggTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCgg < 2:4710062/76‑1 (MQ=255) tATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTg < 1:3948168/76‑1 (MQ=255) tATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGATA‑TGCgggg > 1:4063815/1‑74 (MQ=255) tATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGCTGGTA‑TGTgggg > 1:3022978/1‑74 (MQ=255) aTTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTgc < 2:483343/76‑1 (MQ=255) aTTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATA‑TTCggggg > 2:765587/1‑73 (MQ=255) aTTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGCGCGGTA‑TGCggggg > 1:2832925/1‑73 (MQ=255) tCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGCTGATA‑TGCGGTgcgg > 2:1484292/1‑75 (MQ=255) cacaTCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGTAA‑TGCGGGGggcgg > 1:2820835/1‑75 (MQ=255) cacaTCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGGTA‑TGCGGGGggcgg > 2:2169002/1‑75 (MQ=255) cacaTCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGCATGAAATTGCGG‑GCGCGa > 2:2508737/1‑76 (MQ=255) caTCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGCTGATA‑TGCGGTGCGCGaaa > 2:1184628/1‑76 (MQ=255) aTCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGATA‑TTCGGTGCGCGgaaa > 2:2689191/1‑75 (MQ=255) aTCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGCGTGATA‑TGCGGGGGGCGAAAc > 1:3447462/1‑76 (MQ=255) tCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACt < 2:3731491/76‑1 (MQ=255) tCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACt < 1:792111/76‑1 (MQ=255) tCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGGTA‑TGGGGTGGGCGGAAcc > 2:3172703/1‑75 (MQ=255) tCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATA‑TGCGGGGGGCGAAAcc > 2:3433243/1‑75 (MQ=255) tCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGCATGATA‑TGGGGGGGGCGGAACt > 2:1035123/1‑76 (MQ=255) cGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTc < 2:4325837/76‑1 (MQ=255) cGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTc < 2:4548604/76‑1 (MQ=255) cGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGGTG‑TGTGGTGGGCGGAACTc > 1:1525066/1‑76 (MQ=255) cGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATA‑TGCGGGGCGCGGAACTc > 1:922282/1‑76 (MQ=255) gCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCa < 2:3735045/76‑1 (MQ=255) gCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGATA‑TGCGGGGGGGGAAACTCa > 1:2116228/1‑76 (MQ=255) ccATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCaa < 2:926741/76‑1 (MQ=255) cATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAAt < 1:748627/76‑1 (MQ=255) gAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTcc < 1:1123178/76‑1 (MQ=255) gAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTcc < 1:1162289/76‑1 (MQ=255) aaaGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCa < 2:890465/76‑1 (MQ=255) aaaGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGTTA‑TTCGGTGCGCGAAACTCAACCTCCa > 2:4356515/1‑76 (MQ=255) aaGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCAt < 2:11086/76‑1 (MQ=255) aaGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCAt < 1:2222576/76‑1 (MQ=255) aaGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGCAGGAAA‑TGCGGGGCGCGAAACCCCATCTccct > 2:2773546/1‑74 (MQ=255) ggCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATcc < 2:246881/76‑1 (MQ=255) gCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCg < 2:1229241/76‑1 (MQ=255) gCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCg < 1:887638/76‑1 (MQ=255) cacaTTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGc > 2:4126450/1‑76 (MQ=255) cacaTTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGATA‑TGCGGGGGGGGGAACTCAATCTCCCTCCGc > 1:3424559/1‑76 (MQ=255) cacaTTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGATA‑TGCGGGGGGCGGAAATCAATCTCCATCCGc > 1:353036/1‑76 (MQ=255) cacaTTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATA‑TGAGGTGCGCGAAACTCAAACTCCAccccc > 2:3414659/1‑74 (MQ=255) cacaTTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATA‑AGCGGGGGGCGCAACTCAATCTCCATcccc > 1:923570/1‑74 (MQ=255) cacaTTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGACGGTA‑TGCGGTGCGCGCAACTCCATATCCCTcccc > 2:2489130/1‑74 (MQ=255) acaTTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCt < 2:635555/76‑1 (MQ=255) acaTTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGCATGATA‑TGCGGGGGGTGAAACTCAATCTCCATCCGCt > 1:4861799/1‑76 (MQ=255) ttCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGATA‑TGCGGGGCGCGGAAATCAATCTCCACCCGCTggg > 1:4588319/1‑75 (MQ=255) cACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGGTA‑TGCGGGGCGCGGAACTCAAACTCCCTCCCCTggggt > 2:4385784/1‑73 (MQ=255) aCTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTAtt < 2:602527/76‑1 (MQ=255) cTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTc < 2:770449/76‑1 (MQ=255) ttCGACCTGACCATGCCAGTGGCTGGGGGGGGTGGTA‑TGCGGTGGGGGGAACTCAAACTCCCTCCCCTGGGATTcc > 2:1656527/1‑76 (MQ=255) ttCGACCTGACCATGCCAGTGGCTGGGGGGGATGGTA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCCGGGAtttc > 2:2591943/1‑74 (MQ=255) tCGACCTGACCATGCCAGTGGCTGGGGGGGATGATA‑TGCGGGGCGCGCAACTCAATCTTCATCCGCTCGGCTTCCg > 2:1277268/1‑76 (MQ=255) cGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGa < 2:2201314/76‑1 (MQ=255) gACCTGACCAGGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGaa < 2:3002176/76‑1 (MQ=255) aCCGGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAAt < 1:3841901/76‑1 (MQ=255) tGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATaca < 2:930706/76‑1 (MQ=255) tGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATaca < 1:4851377/76‑1 (MQ=255) cATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagcga < 1:1476873/76‑1 (MQ=255) cATGCCAGTGGCTGGGGGGGGTGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGACTacagcgc > 2:2418043/1‑75 (MQ=255) aTGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagcgac < 1:3609272/76‑1 (MQ=255) tGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagcgaca < 1:1632567/76‑1 (MQ=255) tGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagcgaca < 1:4177384/76‑1 (MQ=255) gCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagcgacag < 1:3768112/76‑1 (MQ=255) ccAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagcgacagc < 2:2116228/76‑1 (MQ=255) aggggCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCgg < 2:3402670/73‑1 (MQ=255) ggCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCt < 1:2845359/76‑1 (MQ=255) gCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTg > 2:3279730/1‑76 (MQ=255) gCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTg > 2:2811887/1‑76 (MQ=255) gCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTg < 2:644526/76‑1 (MQ=255) cTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGc > 1:3959407/1‑76 (MQ=255) cTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGc > 1:2265060/1‑76 (MQ=255) cTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGc > 2:483001/1‑76 (MQ=255) ggtggGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCgg > 2:2034801/1‑76 (MQ=255) ggtggGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCgg > 2:4184899/1‑76 (MQ=255) ggtggGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGAATTCCGAATCCAGCGACAGCGGGCTGCgg > 1:1242153/1‑76 (MQ=255) gtggGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCggg > 1:3718760/1‑76 (MQ=255) gtggGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCggg > 2:1719350/1‑76 (MQ=255) gtggGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCggg > 1:2154461/1‑76 (MQ=255) | TTTCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATA‑TGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGG > REL606/4320150‑4320299 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |