BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	438.3 / NA	119	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (28/91); total (28/91)

Only 100 of 137 total aligned reads displayed.

GATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193709‑2193850

gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                    <  2:2746433/76‑1 (MQ=255)

gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCCGGCCGGAAACAGACCCCAAAAAAGGAAAAAACCAAAccccc                                                                    >  2:1743170/1‑75 (MQ=255)

 aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                                   <  2:2188022/76‑1 (MQ=255)

  tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc                                                                  <  1:3758832/76‑1 (MQ=255)

  tGCATCCGGCGTCCAGGCGCGGCTGGGCCGGGAGTCGGGAATCAGACCCCATAACAACAAAAAGCCCACcccccac                                                                  >  1:4864836/1‑73 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGGCTGGGTGTGGGGAAACAGCCCCCAAAAAAACAAAACGACAAAACCCCCCAGa                                                               >  2:1935028/1‑76 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGGCCCCATAACAGCCAAACGCCCAACCCCACCCGa                                                               >  1:672798/1‑76 (MQ=255)

     aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAAAAGACCCAAAAACAGCAAAAAGCCCATCcccccccca                                                               >  2:2906018/1‑73 (MQ=255)

      tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                              <  1:616001/76‑1 (MQ=255)

      tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGGTGGGAATCAAACCCCATTAAAGCCAAAACCCAAACCCCCCCAgca                                                              >  2:3386119/1‑74 (MQ=255)

      cccGGCGTCCAGGCGCGGCTGGTCTGTCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                              <  1:4362173/75‑1 (MQ=255)

       ccGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCAATAAAAGCAAAACGCCAAACCCCACCAGATa                                                             >  1:1988587/1‑76 (MQ=255)

        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCGATAACAGCAAAACGCCAATCCCCACCAGATaa                                                            <  1:2364614/76‑1 (MQ=255)

        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                            <  1:3371569/76‑1 (MQ=255)

        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                            <  1:4711271/76‑1 (MQ=255)

        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                            <  1:2991837/76‑1 (MQ=255)

         ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                           <  1:3495852/76‑1 (MQ=255)

         ggCGTCCAGGCGCGGCTGGGCTGGCTGTCGGAAATCAGCCCCAATAACAGAAAAACGCCAATCCCCCACCGATaaa                                                           >  1:1227619/1‑76 (MQ=255)

          gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                                                          <  1:4883694/76‑1 (MQ=255)

          gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                                                          <  1:1665470/76‑1 (MQ=255)

             tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt                                                       <  2:3997907/76‑1 (MQ=255)

             tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt                                                       <  2:132706/76‑1 (MQ=255)

              ccAGGCGCGGCTGGTCTGGCTGTCGGGAATCAACCCCAATAAAAACAAAAAGCCAAATCCCAACAGATAAAGCATg                                                      >  2:2377792/1‑76 (MQ=255)

              ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      <  2:2842637/76‑1 (MQ=255)

              ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      <  1:3900425/76‑1 (MQ=255)

               cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                     <  1:4973060/76‑1 (MQ=255)

               cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                     <  2:3173605/76‑1 (MQ=255)

                 ggCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg                                                   <  1:328202/76‑1 (MQ=255)

                    gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                <  1:3479050/76‑1 (MQ=255)

                     cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                               <  2:4975000/76‑1 (MQ=255)

                     cgGCTGGGCTGGCTGTCGGGAATCAGCCCCCATAACAGCAAAACGCCAATCCCCACCCGATAAAACATGACGCCTg                                               >  2:2104824/1‑76 (MQ=255)

                      ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                              >  2:4018910/1‑76 (MQ=255)

                      ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                              <  1:2403266/76‑1 (MQ=255)

                      ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                              <  1:2781925/76‑1 (MQ=255)

                      ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAAGCCTGt                                              >  2:1249777/1‑76 (MQ=255)

                      ggCTGGTCTGGCTGGCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCCCCAGAGAAAGCATAAAGCCCGt                                              >  2:917180/1‑76 (MQ=255)

                        cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                            >  1:1831683/1‑76 (MQ=255)

                         tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  2:583133/76‑1 (MQ=255)

                         tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  1:1330353/76‑1 (MQ=255)

                         tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  2:1040067/76‑1 (MQ=255)

                         tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  2:2543668/76‑1 (MQ=255)

                         tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  1:5027832/76‑1 (MQ=255)

                         tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  1:3773465/76‑1 (MQ=255)

                          ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          >  2:1008314/1‑76 (MQ=255)

                          ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          <  2:4673614/76‑1 (MQ=255)

                           ggcTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAACCCCCACCAGAAAAAGCATGACGCCTGTATTaa                                         <  1:906863/74‑1 (MQ=255)

                           gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                         >  1:2609311/1‑76 (MQ=255)

                           gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                         <  2:3219004/76‑1 (MQ=255)

                            tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                        <  1:3324624/76‑1 (MQ=255)

                              tGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      <  1:227734/76‑1 (MQ=255)

                               ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     >  2:2977688/1‑76 (MQ=255)

                               ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     <  1:1079176/76‑1 (MQ=255)

                               agCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     <  1:2224128/75‑1 (MQ=255)

                                gCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                    <  2:4242229/76‑1 (MQ=255)

                                gCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                    <  1:2420002/76‑1 (MQ=255)

                                 cTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                   >  2:1116991/1‑76 (MQ=255)

                                  tGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                  >  2:4209527/1‑76 (MQ=255)

                                  tGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                  >  2:1184722/1‑76 (MQ=255)

                                   gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 <  2:1393610/76‑1 (MQ=255)

                                   gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 <  2:4289446/76‑1 (MQ=255)

                                   gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 <  2:4529254/76‑1 (MQ=255)

                                   gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 <  2:1233683/76‑1 (MQ=255)

                                   gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 <  2:2718883/76‑1 (MQ=255)

                                   gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 <  2:1537963/76‑1 (MQ=255)

                                    tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                <  2:1033544/76‑1 (MQ=255)

                                     cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                               <  2:529358/76‑1 (MQ=255)

                                     cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                               >  2:2641211/1‑76 (MQ=255)

                                     cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                               <  2:194648/76‑1 (MQ=255)

                                      ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                              <  2:1708751/76‑1 (MQ=255)

                                      ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                              <  2:1543860/76‑1 (MQ=255)

                                        aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                            >  2:890301/1‑76 (MQ=255)

                                          aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                          <  2:1443889/76‑1 (MQ=255)

                                          aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                          <  2:3686920/76‑1 (MQ=255)

                                            cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                        <  1:3123513/76‑1 (MQ=255)

                                             aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                       >  1:1677831/1‑76 (MQ=255)

                                              gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                      <  2:1444857/76‑1 (MQ=255)

                                               ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                     >  2:1747934/1‑76 (MQ=255)

                                               ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                     <  2:387798/76‑1 (MQ=255)

                                                cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                    >  2:1168635/1‑76 (MQ=255)

                                                cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                    <  2:4690444/76‑1 (MQ=255)

                                                 ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                   <  2:1831811/76‑1 (MQ=255)

                                                  cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                  <  2:3439234/76‑1 (MQ=255)

                                                  cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                  <  2:3736470/76‑1 (MQ=255)

                                                   aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                 <  1:2086061/76‑1 (MQ=255)

                                                     tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg               <  2:4995738/76‑1 (MQ=255)

                                                        cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc            <  2:2860279/76‑1 (MQ=255)

                                                         aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgcc           >  2:348323/1‑75 (MQ=255)

                                                         aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa           <  1:905671/76‑1 (MQ=255)

                                                          gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc          <  1:4354634/76‑1 (MQ=255)

                                                           cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc         <  2:173197/76‑1 (MQ=255)

                                                           cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc         <  1:1721125/76‑1 (MQ=255)

                                                           cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc         >  2:3036707/1‑76 (MQ=255)

                                                            aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa        >  2:2956365/1‑76 (MQ=255)

                                                            aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa        >  2:2946785/1‑76 (MQ=255)

                                                            aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa        <  2:2760967/76‑1 (MQ=255)

                                                            aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa        >  2:164606/1‑76 (MQ=255)

                                                             aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg       >  1:2653104/1‑76 (MQ=255)

                                                             aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg       >  1:3440846/1‑76 (MQ=255)

                                                               aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc     >  2:728577/1‑76 (MQ=255)

                                                                ccccAATCCCCACCAGATAAAGCATGACGCTTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg    <  1:4749512/74‑1 (MQ=255)

GATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193709‑2193850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`