| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,261,861 | G→A | F103F (TTC→TTT) | hflB ← | protease, ATP‑dependent zinc‑metallo |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,261,861 | 0 | G | A | 99.2% | 500.8 / ‑3.6 | 123 | F103F (TTC→TTT) | hflB | protease, ATP‑dependent zinc‑metallo |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (69/53); total (70/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
CACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTA > REL606/3261788‑3261933 | ccccgccgCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATaaa < 1:2900732/74‑1 (MQ=255) aCCGCCGCCCTGCATTTGCCGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAg < 2:4723678/76‑1 (MQ=255) aCCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAg < 1:3095056/76‑1 (MQ=255) aCCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAg > 1:2669590/1‑76 (MQ=255) aCCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAg > 2:4250170/1‑76 (MQ=255) aCCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAg > 1:4062453/1‑76 (MQ=255) ccgccgCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGa > 1:3960541/1‑76 (MQ=255) cgccgcCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGAt < 1:1820431/76‑1 (MQ=255) cgccgcCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGAt < 1:2379830/76‑1 (MQ=255) gccgccCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATa < 1:3526704/76‑1 (MQ=255) gccgccCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATa < 2:1972522/76‑1 (MQ=255) ccgccCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAg > 1:596768/1‑76 (MQ=255) ccgccCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAg < 1:2198609/76‑1 (MQ=255) cgccCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGa > 2:2537577/1‑76 (MQ=255) cgccCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGa > 1:3366181/1‑76 (MQ=255) gccCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGaa < 1:2232675/76‑1 (MQ=255) gccCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGaa < 1:3352636/76‑1 (MQ=255) cccTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAg > 1:1381567/1‑76 (MQ=255) cTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGcc > 2:1622395/1‑76 (MQ=255) gCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCcag > 2:1995825/1‑76 (MQ=255) gCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCcag < 1:2845628/76‑1 (MQ=255) aTTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCcagca > 2:271916/1‑76 (MQ=255) aTTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCcagca > 2:1749194/1‑76 (MQ=255) tttGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCcagcag > 1:897088/1‑76 (MQ=255) ttGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAgg < 1:3826857/76‑1 (MQ=255) tGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGc > 2:2544337/1‑76 (MQ=255) gACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCt > 1:4776869/1‑76 (MQ=255) gACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCt > 1:1239506/1‑76 (MQ=255) cGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTg > 1:2973783/1‑76 (MQ=255) cGCAGGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTg < 2:1807290/76‑1 (MQ=255) gCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTgg < 1:1935572/76‑1 (MQ=255) aTGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGtt > 2:4477978/1‑76 (MQ=255) aTGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGtt > 2:3770964/1‑76 (MQ=255) tGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGttc > 1:2601628/1‑76 (MQ=255) tGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGttc < 2:3633649/76‑1 (MQ=255) gaagaaGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGttct > 1:626034/1‑76 (MQ=255) agaagaTCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGttcttc > 2:2292418/1‑76 (MQ=255) gaagaTCCCGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCa < 1:4102643/76‑1 (MQ=255) gaaaaTCCAGAACCCATGAAGAAACAA‑CGTCGGGAACCAGGAGATAAAGATAAAAGCCAGCAGGCTTGGTTCTTCa < 2:3806922/76‑1 (MQ=255) aagaTCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAg < 2:118664/76‑1 (MQ=255) ccAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGt > 2:4004612/1‑76 (MQ=255) ccAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGt > 2:1346603/1‑76 (MQ=255) cAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGtt > 1:1600789/1‑76 (MQ=255) cAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGtt > 1:1247008/1‑76 (MQ=255) aGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTc < 1:1983444/76‑1 (MQ=255) aGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTc < 1:3160451/76‑1 (MQ=255) acacCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCAc < 2:4833893/76‑1 (MQ=255) cacCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCAcc < 2:2396125/76‑1 (MQ=255) acCAATCAGCAACAG‑CATGGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCg < 2:300504/76‑1 (MQ=255) acCAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCg < 1:2310037/76‑1 (MQ=255) ccAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGa < 2:3751051/76‑1 (MQ=255) ccAATCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGa > 2:1717041/1‑76 (MQ=255) aaccAGAAACAG‑CATCGGAAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACa < 1:3112936/73‑1 (MQ=255) aaTCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACa < 1:1983064/76‑1 (MQ=255) aTCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTTACCGACaa > 1:2126851/1‑76 (MQ=255) aTCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACaa > 2:2890677/1‑76 (MQ=255) aTCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACaa < 2:3058911/76‑1 (MQ=255) aTCAGC‑ACAGCCATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACaa < 2:330590/76‑1 (MQ=255) tCAGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAAc < 2:182359/76‑1 (MQ=255) cAGAAACAG‑CATCGGGAACCAGGAGATAAGGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAAcc < 2:1227766/76‑1 (MQ=255) agaaaCAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCt < 1:4180391/73‑1 (MQ=255) aGCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCt > 2:4057387/1‑76 (MQ=255) gaaaCAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCtt < 1:687129/74‑1 (MQ=255) gCAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCtt < 1:3500335/76‑1 (MQ=255) cAACAG‑CATGGGAAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTg < 1:3733280/76‑1 (MQ=255) cAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTg > 1:878312/1‑76 (MQ=255) cAACAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTg > 1:4039332/1‑76 (MQ=255) aaCAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGa > 2:1042140/1‑76 (MQ=255) aaCAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGa > 2:4709794/1‑76 (MQ=255) aaCAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGa > 1:4302741/1‑76 (MQ=255) aaCAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGa > 2:243120/1‑76 (MQ=255) cAG‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACg < 1:4925670/76‑1 (MQ=255) g‑CATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGtt < 2:4515775/76‑1 (MQ=255) cATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTc > 1:2409924/1‑76 (MQ=255) cATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTc > 1:3001986/1‑76 (MQ=255) cATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTc > 2:2804707/1‑76 (MQ=255) cATCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTc > 1:1563132/1‑76 (MQ=255) aTCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACGTTGACGTTCt > 2:2990946/1‑76 (MQ=255) aTCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCt > 2:4943691/1‑76 (MQ=255) aTCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCt > 2:4416675/1‑76 (MQ=255) tCGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCtt < 2:3468848/76‑1 (MQ=255) cGGGAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTg > 2:2048945/1‑76 (MQ=255) gggAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTgg < 1:756331/76‑1 (MQ=255) gggAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTgg < 2:4560931/76‑1 (MQ=255) gggAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTgg > 2:2588261/1‑76 (MQ=255) gAACCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTc < 1:4444881/76‑1 (MQ=255) aCCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCaa > 1:4286605/1‑76 (MQ=255) aCCAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCaa < 2:2836900/76‑1 (MQ=255) ccAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAAc < 1:417157/76‑1 (MQ=255) ccAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAAc < 1:1727947/76‑1 (MQ=255) cAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACa > 1:1747405/1‑76 (MQ=255) cAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACa > 2:2901920/1‑76 (MQ=255) cAGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACa < 2:1560887/76‑1 (MQ=255) aGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAg < 1:358308/76‑1 (MQ=255) aGGAGATAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAg > 1:3686244/1‑76 (MQ=255) gagaTAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGt > 2:2698375/1‑76 (MQ=255) agaTAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGtt < 1:3410492/76‑1 (MQ=255) agaTAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGtt > 1:1170147/1‑76 (MQ=255) agaTAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGtt > 1:4295539/1‑76 (MQ=255) gaTAAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTa > 1:729243/1‑76 (MQ=255) | CACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAG‑CATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTA > REL606/3261788‑3261933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |