| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,464,820 | C→T | R150H (CGC→CAC) | ompR ← | osmolarity response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,464,820 | 0 | C | T | 98.8% | 343.7 / ‑3.4 | 84 | R150H (CGC→CAC) | ompR | osmolarity response regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (16/67); total (16/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01 | |||||||||||
GACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGCGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCAGTT > REL606/3464750‑3464892 | gcccAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑a < 1:1331738/74‑1 (MQ=255)gACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑a < 1:4216740/76‑1 (MQ=255)gACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCCCCCTCCC‑GGAACACTTCTTGCGtc > 2:1786309/1‑75 (MQ=255) cAGTGCCTTCAGTCCCGCAAACTCACCGCTGGTGTGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCg < 1:1791464/76‑1 (MQ=255) cAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCg < 2:2162085/76‑1 (MQ=255) gCCTTCAGTACCGCAACCTCACCGCTGGGGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGt < 1:4759291/76‑1 (MQ=255) ctttCAGTACCGCAACCTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGtt < 1:69944/74‑1 (MQ=255) ccTTCAGTCCCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGtt < 1:144374/76‑1 (MQ=255) ccTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGtt < 1:4712362/76‑1 (MQ=255) ccTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGtt < 1:4972788/76‑1 (MQ=255) ccTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGtt < 1:2344899/76‑1 (MQ=255) cTTCAGTACCGCAACCTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTa < 1:3972086/76‑1 (MQ=255) cTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTa < 1:4792287/76‑1 (MQ=255) cTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTa < 2:101164/76‑1 (MQ=255) ttCAGTACCGCAAACTCCCCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTaa < 2:4106327/76‑1 (MQ=255) ttCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTaa < 1:4577935/76‑1 (MQ=255) ttCAGTACCGCAAACTCACCGCTGGGGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTaa < 2:2452739/76‑1 (MQ=255) tCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAg < 2:4833895/76‑1 (MQ=255) cAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGt < 1:4849902/76‑1 (MQ=255) cAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGt < 2:1112702/76‑1 (MQ=255) cAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGt < 2:3166136/76‑1 (MQ=255) aGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGtt < 1:93145/76‑1 (MQ=255) aGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGCGCGT‑ACCGAGGTTAAGtt < 2:1029725/76‑1 (MQ=255) gtcccGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGttt < 2:2218881/73‑1 (MQ=255) gTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGttt < 1:4839860/76‑1 (MQ=255) gTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGttt < 2:4533535/76‑1 (MQ=255) cGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAAc < 1:1503702/76‑1 (MQ=255) cAAAGTCCCCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACtt < 1:1071954/76‑1 (MQ=255) cAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACtt < 1:3115468/76‑1 (MQ=255) cAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACtt < 2:3384700/76‑1 (MQ=255) aaaCTCACCGCTGGTTGGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTa < 1:3638977/76‑1 (MQ=255) aaaCTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTa < 2:1210783/76‑1 (MQ=255) aaaCTCACCGCTGGTGAGCGGCATCGGCTCCCCTTCCC‑CGGACAATTCTTGCGTGCCCCGGGTTAAGTTTTAAttt > 2:4106687/1‑76 (MQ=255) aaaCTCACCGCTGGTGAGCGGCATCGGCTCCCCCTCGC‑CGGATATTTCCTGCGT‑GCCCGGGGTAAACTTGAAAttt > 2:3574776/1‑75 (MQ=255) aaaCTCACCGCTGGTGAGCGGCATCGGCTCCCCCTCCC‑GGGGCACTTTTTGGGT‑ACCGAGGGTAAGTTTGTACttt > 2:635384/1‑75 (MQ=255) aaaCTCACCGCTGGTGAGCGGCATCGGCTCCCCCTCCC‑CGGGCGCTTCGCGAGT‑ACCGAGGTTAAAGTTGAAAttt > 2:3411229/1‑75 (MQ=255) aaaCTCACCGCTGGTGAGCGGCATCGGCTCCCCCTCCC‑CGGACACTTCTTGCGG‑ACCGAGGGTGAGTTTTTACttt > 2:4475671/1‑75 (MQ=255) aaCTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTAc < 1:4505067/76‑1 (MQ=255) cTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCg < 1:4584308/76‑1 (MQ=255) cTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCg < 1:1234395/76‑1 (MQ=255) cTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCg < 2:3153567/76‑1 (MQ=255) tCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGa < 2:2612747/76‑1 (MQ=255) tCACCGCTGGTGAGCGGCATCGGCTCCCCCTCGCGGGAACTTTTTTTGCG‑‑ACCGAGGGTAAGGTTTGACTTAcagc > 2:1959381/1‑73 (MQ=255) ccGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAg < 2:2599683/76‑1 (MQ=255) ccGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAg < 2:3710667/76‑1 (MQ=255) gCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCa < 2:4212001/76‑1 (MQ=255) cTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCaa < 1:4477041/76‑1 (MQ=255) tGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAAt < 2:2910582/76‑1 (MQ=255) ggTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAAtt < 1:3893280/76‑1 (MQ=255) ggTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAAtt < 1:1111913/76‑1 (MQ=255) ggTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAAtt < 1:3350844/76‑1 (MQ=255) ggTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGCTTGAACTTACCGAAAGCAAtt < 2:1423490/76‑1 (MQ=255) gtgtGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTa < 1:813274/76‑1 (MQ=255) gTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTa < 1:2711555/76‑1 (MQ=255) gTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTa < 2:4551747/76‑1 (MQ=255) gTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTa < 1:2343964/76‑1 (MQ=255) tGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTAc < 1:4330395/76‑1 (MQ=255) tGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTAc < 1:3547165/76‑1 (MQ=255) tGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTAc < 1:37041/76‑1 (MQ=255) aGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCg < 1:660168/76‑1 (MQ=255) gCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGc < 2:3907579/76‑1 (MQ=255) gCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGc < 1:2645637/76‑1 (MQ=255) ggCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCct < 1:1211482/76‑1 (MQ=255) gCATCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCctc < 2:3935559/76‑1 (MQ=255) aTCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCtt < 1:3952405/76‑1 (MQ=255) tCGGCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTc < 1:1012720/76‑1 (MQ=255) ggCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCt < 1:1395052/76‑1 (MQ=255) gCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCtg < 1:550727/76‑1 (MQ=255) gCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCtg < 2:2744076/76‑1 (MQ=255) gCTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCtg < 1:4701973/76‑1 (MQ=255) cTCGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCtgt < 1:4543986/76‑1 (MQ=255) cGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGa < 2:701833/76‑1 (MQ=255) cGTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGa < 1:4322709/76‑1 (MQ=255) gTCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGAc > 1:3477390/1‑76 (MQ=255) tCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACg > 2:148954/1‑76 (MQ=255) tCTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACg > 2:847764/1‑76 (MQ=255) cTTCGC‑GGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACgg > 1:4290246/1‑76 (MQ=255) cgc‑gGAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGgtgt < 2:1199376/76‑1 (MQ=255) ggAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGcc > 2:48779/1‑76 (MQ=255) gAACATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCt > 1:1537642/1‑76 (MQ=255) aaCATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTg > 2:273067/1‑76 (MQ=255) cATTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGc > 1:690727/1‑76 (MQ=255) aTTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCa > 2:2774449/1‑76 (MQ=255) aTTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCa > 2:4002646/1‑76 (MQ=255) aTTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCa < 1:1922276/76‑1 (MQ=255) aTTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCa > 1:4991435/1‑76 (MQ=255) aTTTCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCa > 1:4758355/1‑76 (MQ=255) tttCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCAg > 1:2090163/1‑76 (MQ=255) tttCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCAg > 1:486193/1‑76 (MQ=255) ttCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCAgt > 1:1500212/1‑76 (MQ=255) tCGTGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCAgtt > 1:2209953/1‑76 (MQ=255) | GACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCTCGTCTTCGC‑GGAACATTTCGCGCGT‑ACCGAGGTTAAGTTTGAACTTACCGAAAGCAATTACCGCCTCTTCCTGTGACGGTGTGCCTGGCAGTT > REL606/3464750‑3464892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |