Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	743,241	A→G	K310R (AAG→AGG)	sucB →	dihydrolipoamide acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	743,241	0	A	G	100.0%	367.4 / NA	86	K310R (AAG→AGG)	sucB	dihydrolipoamide acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/48);  total (38/48)

ATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATG  >  REL606/743169‑743311                                                                         |                                                                       aTGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCt                                                                     <  1:2677272/76‑1 (MQ=255)  tGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTg                                                                    <  2:4329973/76‑1 (MQ=255)  tGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTg                                                                    <  2:2215173/76‑1 (MQ=255)   gTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGa                                                                   >  1:4006423/1‑76 (MQ=255)       aTACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGGTGACCGt                                                               >  2:2333812/1‑76 (MQ=255)        tACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGAGCgcg                                                              >  1:3064618/1‑74 (MQ=255)          cccTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGa                                                            <  1:461357/76‑1 (MQ=255)          cccTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGa                                                            <  2:1558067/76‑1 (MQ=255)           ccTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGaa                                                           <  1:1093296/76‑1 (MQ=255)           ccTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGaa                                                           <  1:1287621/76‑1 (MQ=255)            cTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAg                                                          <  1:1887319/76‑1 (MQ=255)            cTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAg                                                          <  1:2111335/76‑1 (MQ=255)              cGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGAt                                                        <  2:3614736/76‑1 (MQ=255)               ggCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATc                                                       >  1:4102864/1‑76 (MQ=255)               ggCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATc                                                       <  2:2477069/76‑1 (MQ=255)               ggCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATc                                                       <  2:3000776/76‑1 (MQ=255)                 cATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTg                                                     <  1:629192/76‑1 (MQ=255)                 cATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTg                                                     <  1:3323432/76‑1 (MQ=255)                 cATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTg                                                     >  1:1103536/1‑76 (MQ=255)                 cATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTg                                                     <  2:4939147/76‑1 (MQ=255)                 cATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTg                                                     <  1:1826479/76‑1 (MQ=255)                 cATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGGTGAAGATCtc                                                     >  2:4752064/1‑75 (MQ=255)                 cATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCCGTGGAAATCTg                                                     >  1:325823/1‑76 (MQ=255)                 cATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGAACGGTTAAGATccg                                                     >  1:151209/1‑74 (MQ=255)                  aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGa                                                    <  1:4828782/76‑1 (MQ=255)                  aTGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGa                                                    <  2:3635341/76‑1 (MQ=255)                    ggCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGAcc                                                  >  1:929511/1‑76 (MQ=255)                      cAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCgg                                                >  2:3681608/1‑76 (MQ=255)                       aGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGGCCGTTGAAAATCTGACCggg                                               >  2:3787999/1‑75 (MQ=255)                       aGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCggt                                               <  1:1296583/76‑1 (MQ=255)                          cATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGGGACGGCAGGCTGTCCGGTGAAGGTCTGACCggtggc                                            >  2:1963619/1‑75 (MQ=255)                           aTCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTa                                           <  1:4475788/76‑1 (MQ=255)                           aTCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTa                                           <  1:3056201/76‑1 (MQ=255)                            tCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTaa                                          <  2:3686437/76‑1 (MQ=255)                            tCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTaa                                          <  2:718253/76‑1 (MQ=255)                             cGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAAc                                         <  1:3158545/76‑1 (MQ=255)                             cGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGGGGTAAc                                         >  2:3740747/1‑76 (MQ=255)                             cGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGGGGTAAc                                         >  1:4970557/1‑76 (MQ=255)                             cGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGGGGTAAc                                         >  1:726334/1‑76 (MQ=255)                             cGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGGTGAAGATCTGACCCGTGGTAAc                                         >  2:1547447/1‑76 (MQ=255)                               agaagaAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACtt                                       <  2:2062920/76‑1 (MQ=255)                               agaagaAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACtt                                       <  1:4212512/76‑1 (MQ=255)                               agaagaAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGGAACtt                                       >  1:389053/1‑76 (MQ=255)                                  agaaAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcac                                    <  1:4888166/76‑1 (MQ=255)                                  agaaAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcac                                    <  1:2995420/76‑1 (MQ=255)                                       aTCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccatca                               <  2:358654/76‑1 (MQ=255)                                         cAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccatcacc                             >  2:3443919/1‑76 (MQ=255)                                         cAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGGAACTtcaccatcacc                             >  1:4313908/1‑76 (MQ=255)                                         cAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGGGGGAACTtcaccatccac                             >  1:430661/1‑73 (MQ=255)                                          aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAGGGTGACCCTTGAAGATCTGACCGGTGGTAACTtcaccatcacca                            >  2:2716193/1‑76 (MQ=255)                                          aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccctcacca                            >  2:2658929/1‑76 (MQ=255)                                          aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccatcacca                            <  1:3045640/76‑1 (MQ=255)                                          aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccatcacca                            >  2:3750239/1‑76 (MQ=255)                                          aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccatcacca                            <  1:2214827/76‑1 (MQ=255)                                          aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccatcacca                            <  2:2717430/76‑1 (MQ=255)                                          aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccatcacca                            <  2:3990689/76‑1 (MQ=255)                                          aaaGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTtcaccatcacca                            <  2:82892/76‑1 (MQ=255)                                           aaGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCaa                           <  1:70578/76‑1 (MQ=255)                                           aaGAGCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGGGGTAAATtcaccatcaccca                           >  1:279123/1‑74 (MQ=255)                                            agatCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCCAc                          >  1:3907426/1‑76 (MQ=255)                                            agagCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAAc                          <  1:4658201/76‑1 (MQ=255)                                            agagCTGGCAGTCAAAGGCCGTGACGGCAGGCCGACCGTTGAAGATATGGCCGGGGGGAACTTCACCATCACCAAc                          >  2:576021/1‑76 (MQ=255)                                            agagCTGGCAGTCAAAGGCCGTGACGGCAGGATGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCCAc                          >  2:4922656/1‑76 (MQ=255)                                               gCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggt                       <  2:4294114/76‑1 (MQ=255)                                               gCTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggt                       <  2:1049226/76‑1 (MQ=255)                                                cTGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACggtg                      <  2:4603018/76‑1 (MQ=255)                                                 tGGCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGGAACTTCACCATCACCAACggggg                     >  2:2810503/1‑76 (MQ=255)                                                  ggCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAAAggtggg                    >  1:658118/1‑75 (MQ=255)                                                   gCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCCACggggggg                   >  2:1265520/1‑74 (MQ=255)                                                   gCAGTCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGgtggtg                   <  2:632598/76‑1 (MQ=255)                                                       tCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGGGTGtt               >  1:3391102/1‑76 (MQ=255)                                                       tCAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGGAACTTCACCATCACCAACGGTGGGGTGtt               >  2:196491/1‑76 (MQ=255)                                                        cAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTc              <  1:4445921/76‑1 (MQ=255)                                                        cAAAGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGGAACTTCACCCTCACCAACGGGGGTGTGTTc              >  2:1845097/1‑76 (MQ=255)                                                         aaaGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCg             <  1:3858567/76‑1 (MQ=255)                                                         aaaGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCg             <  1:1887207/76‑1 (MQ=255)                                                          aaGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCgg            <  1:1355562/76‑1 (MQ=255)                                                          aaGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCgg            >  1:4131966/1‑76 (MQ=255)                                                           aGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGt           <  2:1869748/76‑1 (MQ=255)                                                           aGGCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACCTCACCATCACCAACGGGGGGGTGTTCGGt           >  2:2820970/1‑76 (MQ=255)                                                            ggCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGGGGGTTCgggt          >  1:1568372/1‑74 (MQ=255)                                                            ggCCGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGGAACTTCACCATCACCAACGGTGGTGTGTTCGGtt          >  2:3054607/1‑76 (MQ=255)                                                               cGTGACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGGGTGTTCGGTTccc       >  1:3938100/1‑76 (MQ=255)                                                                 ggACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTg     <  2:2463489/75‑1 (MQ=255)                                                                  gACGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTccccgg    >  2:2840161/1‑73 (MQ=255)                                                                   aCGGCAGGGTGACCGTTGGAGATCTGACCGGGGGTAAATTTACCATCACCAACGGGGGGGTGTTCGGGTCCCTgtt   >  2:499349/1‑74 (MQ=255)                                                                   aCGGCAGGCTGACCGTTGAAGATCTTACCGGTGGTAACTTCTCCATCACCAACGGTGGTGTGTTCGGTTCCCTGAt   <  2:2013985/76‑1 (MQ=255)                                                                   aCGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGAt   <  1:2044860/76‑1 (MQ=255)                                                                   aCGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGAt   <  1:4463828/76‑1 (MQ=255)                                                                   aCGGCAGGCTGACCGTTGAAGATCTGACCGGGGGGAACTTCACCATCACCAACGGTGGGGTGTTCGGTTCCCTGAt   >  2:3520640/1‑76 (MQ=255)                                                                    cGGCAGGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATg  >  1:2402503/1‑76 (MQ=255)                                                                         |                                                                       ATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATG  >  REL606/743169‑743311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑