BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,163,752	T→G	I966S (ATC→AGC)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,163,752	0	T	G	100.0%	362.6 / NA	86	I966S (ATC→AGC)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (64/22); total (64/22)

Only 100 of 101 total aligned reads displayed.

ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163680‑4163824

aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                       >  1:2314901/1‑76 (MQ=255)

aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                       <  2:3780358/76‑1 (MQ=255)

aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt                                                                       <  2:2803011/76‑1 (MQ=255)

 cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc                                                                      >  1:16008/1‑76 (MQ=255)

 cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc                                                                      >  1:887065/1‑76 (MQ=255)

  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                     <  2:2227972/76‑1 (MQ=255)

  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                     >  2:4452743/1‑76 (MQ=255)

  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg                                                                     >  2:4886041/1‑76 (MQ=255)

   aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGa                                                                    >  1:4255322/1‑76 (MQ=255)

    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGaa                                                                   >  1:410517/1‑76 (MQ=255)

    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGaa                                                                   <  2:4015923/76‑1 (MQ=255)

    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGaa                                                                   <  2:2514490/76‑1 (MQ=255)

     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg                                                                  <  2:4445540/76‑1 (MQ=255)

     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg                                                                  <  2:856930/76‑1 (MQ=255)

     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg                                                                  <  2:3650387/76‑1 (MQ=255)

     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg                                                                  <  2:1106025/76‑1 (MQ=255)

      gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGc                                                                 >  1:4717231/1‑76 (MQ=255)

       tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCg                                                                <  2:2622472/76‑1 (MQ=255)

       tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCg                                                                <  2:1556831/76‑1 (MQ=255)

        gcgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCgg                                                               <  2:1800672/76‑1 (MQ=255)

         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg                                                              >  1:2917876/1‑76 (MQ=255)

          gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGt                                                             >  1:3041327/1‑76 (MQ=255)

           cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCgggcc                                                            >  2:4233606/1‑74 (MQ=255)

           cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGCGTc                                                            >  2:647605/1‑76 (MQ=255)

            tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt                                                           <  1:3879248/76‑1 (MQ=255)

                 aTCGACGACATCCAGCTCAACCAGGCGAAGACAGCCAAGTCTGAAGAACTGCAGAGCCTGGCAGCGGGTCTGttaa                                                      >  2:4917003/1‑74 (MQ=255)

                 aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa                                                      <  2:2302263/76‑1 (MQ=255)

                 aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCa                                                      >  2:4917002/1‑76 (MQ=255)

                  tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAg                                                     <  2:1123662/76‑1 (MQ=255)

                    gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGcc                                                   <  1:4331645/76‑1 (MQ=255)

                     aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCg                                                  >  2:2043297/1‑76 (MQ=255)

                     aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCg                                                  >  1:4690426/1‑76 (MQ=255)

                     aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCg                                                  >  2:4866413/1‑76 (MQ=255)

                     aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCg                                                  >  2:339701/1‑76 (MQ=255)

                     aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCg                                                  >  2:3860239/1‑76 (MQ=255)

                     aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCg                                                  >  2:1271585/1‑76 (MQ=255)

                        aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTAt                                               >  2:4369358/1‑76 (MQ=255)

                        aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTAt                                               >  2:3215308/1‑76 (MQ=255)

                        aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTAt                                               <  2:3359883/76‑1 (MQ=255)

                        aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTAt                                               >  2:404103/1‑76 (MQ=255)

                        aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTAt                                               >  1:123995/1‑76 (MQ=255)

                         aaTGCAGCTCAAACAGGCGAAGAAAGCCCTGTCGGAAGACCGGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATc                                              <  2:759938/76‑1 (MQ=255)

                         aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATc                                              >  1:2415624/1‑76 (MQ=255)

                          aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATcc                                             <  1:1615927/76‑1 (MQ=255)

                           tGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCg                                            >  1:4301772/1‑76 (MQ=255)

                             cAGCTAAAAGAGGCGAAGAAAGCCTTGTCGAAAGAACTGCAGAGCCGCGAAGGGGGTTTGTCCAGCCGTATCCgtg                                          <  2:4384297/76‑1 (MQ=255)

                               gCTAAAACGGGGGAAAAAACACCTGTCGGAAGAACGGCGGAGCCTGGAAGCGGGTCTGTTCAGCCGTATCCgtgct                                        <  2:3844588/76‑1 (MQ=255)

                                cacAAACAGGCGAAGAAAGCCCGGTCGGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctg                                       <  2:3617701/74‑1 (MQ=255)

                                caaaaaCGGGCGAAAAAAGACCTGTCTGAAGACCTGCAGAGCCGCGAAGCGGGTCTGTTCAGCCGTATCCgtgctg                                       <  2:604415/73‑1 (MQ=255)

                                cTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctg                                       >  2:4649854/1‑76 (MQ=255)

                                 tCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgt                                      >  1:2258643/1‑76 (MQ=255)

                                 tCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgt                                      >  2:2178456/1‑76 (MQ=255)

                                 tCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgt                                      >  2:1264989/1‑76 (MQ=255)

                                 tCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgt                                      >  2:1411987/1‑76 (MQ=255)

                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                     >  2:4384363/1‑76 (MQ=255)

                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                     >  2:3659845/1‑76 (MQ=255)

                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                     <  1:2806681/76‑1 (MQ=255)

                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                     >  1:3576122/1‑76 (MQ=255)

                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg                                     >  1:4203414/1‑76 (MQ=255)

                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCAGTATCCgtgctgag                                     >  1:3236417/1‑74 (MQ=255)

                                   aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgc                                    >  1:396167/1‑76 (MQ=255)

                                    aaCAGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct                                   <  1:3327757/76‑1 (MQ=255)

                                      cgggCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTgg                                 <  1:557688/74‑1 (MQ=255)

                                       aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt                                >  2:1098363/1‑76 (MQ=255)

                                       aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt                                <  1:3163877/76‑1 (MQ=255)

                                        ggCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa                               <  1:2332989/76‑1 (MQ=255)

                                        ggCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa                               >  2:1224938/1‑76 (MQ=255)

                                        ggCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTa                               >  2:1650933/1‑76 (MQ=255)

                                         gCGAAGAAAGCCCTGTCTGAAGAACGGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg                              <  2:447067/76‑1 (MQ=255)

                                         gCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg                              >  2:819034/1‑76 (MQ=255)

                                         gCGAAAAAAGCCCTGCCGAAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg                              <  2:151256/76‑1 (MQ=255)

                                            aagaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg                           >  2:2739730/1‑76 (MQ=255)

                                             agaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCgg                          >  2:2229555/1‑76 (MQ=255)

                                             agaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCgg                          >  2:412897/1‑76 (MQ=255)

                                              gaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGt                         >  1:1955813/1‑76 (MQ=255)

                                              gaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCCGt                         >  1:3122293/1‑76 (MQ=255)

                                               aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCgggg                        >  1:4560038/1‑74 (MQ=255)

                                               aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  2:911983/1‑76 (MQ=255)

                                               aaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  2:4134642/1‑76 (MQ=255)

                                               aaagaACTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTATGTTCGGACGTATCCGTGCTGTGCTGGTAGTCggcg                        >  2:911984/1‑74 (MQ=255)

                                                      tGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa                 >  2:2151161/1‑76 (MQ=255)

                                                      tGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa                 >  2:404886/1‑76 (MQ=255)

                                                       gTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa                >  2:249632/1‑76 (MQ=255)

                                                         cTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  1:2630139/1‑76 (MQ=255)

                                                          tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt             >  2:2541557/1‑76 (MQ=255)

                                                           gaagaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  2:1049980/1‑76 (MQ=255)

                                                            aagaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGCAGCTga           >  2:1385336/1‑76 (MQ=255)

                                                            aagaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga           >  2:4773343/1‑76 (MQ=255)

                                                             agaaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag          >  2:2543726/1‑76 (MQ=255)

                                                               aaCTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa        >  1:2751284/1‑76 (MQ=255)

                                                                ccTGCGGAGCCTGGAAGGGGGTCTGTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg       <  1:3803219/75‑1 (MQ=255)

                                                                 cTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAga      >  2:3565170/1‑75 (MQ=255)

                                                                 cTGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGc      >  2:1545587/1‑76 (MQ=255)

                                                                  tGCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     >  1:1814763/1‑76 (MQ=255)

                                                                   gCAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc    >  1:979845/1‑76 (MQ=255)

                                                                    cAGAGCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   >  1:3692289/1‑76 (MQ=255)

                                                                     agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  1:3714639/1‑76 (MQ=255)

                                                                     agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  2:4524426/1‑76 (MQ=255)

                                                                     agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  2:3242308/1‑76 (MQ=255)

                                                                     agagCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGGGGCGTTGAAGCTGAGAAGCTCGa  >  2:4638641/1‑76 (MQ=255)

ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163680‑4163824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`