| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,201,960 | A→C | D200E (GAT→GAG) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,201,960 | 0 | A | C | 98.7% | 653.9 / ‑3.0 | 154 | D200E (GAT→GAG) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (83/69); total (83/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
CTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAG > REL606/4201887‑4202031 | tGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGctct < 1:3282471/76‑1 (MQ=255) tGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtca < 2:2928309/76‑2 (MQ=255) gCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAGTAACCCCGTTTGCGCGTTTGGGCGAGCtctt < 2:3544363/76‑1 (MQ=255) gCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtctt < 2:1433344/76‑1 (MQ=255) cAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtcttc > 2:3493491/1‑76 (MQ=255) aaGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtcttct < 1:1005882/76‑1 (MQ=255) aaGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtcttct > 1:2470269/1‑76 (MQ=255) aaGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtcttct > 1:484814/1‑76 (MQ=255) aaGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtctcct > 2:4999663/1‑76 (MQ=255) aGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTcttctt < 2:2048356/76‑1 (MQ=255) ggCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCttt < 2:1056942/76‑1 (MQ=255) gCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCtttt < 2:723374/76‑1 (MQ=255) cAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTa < 1:685044/76‑1 (MQ=255) aaCGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTaa < 2:4349314/76‑1 (MQ=255) aaCGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTaa < 1:3772948/76‑1 (MQ=255) aCGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTaaa > 1:2231895/1‑76 (MQ=255) cGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAAt > 1:1067545/1‑76 (MQ=255) gTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATg > 2:624147/1‑76 (MQ=255) tAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGc < 1:2683573/76‑1 (MQ=255) aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 1:1863041/1‑76 (MQ=255) aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 1:617953/1‑76 (MQ=255) aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 1:493170/1‑76 (MQ=255) aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 1:1278386/1‑76 (MQ=255) aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 2:1913227/1‑76 (MQ=255) aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 2:419286/1‑76 (MQ=255) ccccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGcaca < 2:674283/76‑1 (MQ=255) ccccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGcaca > 2:2749079/1‑76 (MQ=255) cccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAg < 1:4172669/76‑1 (MQ=255) ccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAACTCTTCTTTTAAATGCACAgg < 1:4660783/76‑1 (MQ=255) cAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGga > 2:1186693/1‑76 (MQ=255) aGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGgag > 2:747054/1‑76 (MQ=255) aGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGgag < 2:2319680/76‑1 (MQ=255) aGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGgag > 1:1283386/1‑76 (MQ=255) cgcATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGac < 2:4480668/76‑1 (MQ=255) cgcATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGac > 2:2441711/1‑76 (MQ=255) gcATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGaca < 2:1731454/76‑1 (MQ=255) cATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGacac > 1:397175/1‑76 (MQ=255) cATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGacac > 1:641653/1‑76 (MQ=255) cATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGacac > 2:3362567/1‑76 (MQ=255) tGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCa > 1:746656/1‑76 (MQ=255) gTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAg > 1:4528958/1‑76 (MQ=255) gTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAg < 2:2467550/76‑1 (MQ=255) gTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAg > 1:1179115/1‑76 (MQ=255) gTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAg > 1:1467354/1‑76 (MQ=255) tCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCg > 1:1412189/1‑76 (MQ=255) tCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCg > 2:568946/1‑76 (MQ=255) cTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGtt < 2:2751335/76‑1 (MQ=255) tCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTg < 2:2204200/76‑1 (MQ=255) tCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTg < 1:3805966/76‑1 (MQ=255) tCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGt > 1:3522426/1‑76 (MQ=255) cGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTg < 2:822588/76‑1 (MQ=255) gTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGGGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgg > 1:2349215/1‑76 (MQ=255) gTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgg > 1:4050219/1‑76 (MQ=255) gTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgg > 2:2427313/1‑76 (MQ=255) tCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTggg < 2:3877688/76‑1 (MQ=255) tCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTggg > 2:247233/1‑76 (MQ=255) cAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGt < 2:1230270/76‑1 (MQ=255) cAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGt < 1:4166119/76‑1 (MQ=255) aaaTGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGta > 1:2416908/1‑76 (MQ=255) aaaTGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGta < 1:2299279/76‑1 (MQ=255) aTGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGtata < 1:464615/76‑1 (MQ=255) tGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAg > 2:3070142/1‑76 (MQ=255) tGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAg < 1:3856869/76‑1 (MQ=255) gAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAgg > 2:1867983/1‑76 (MQ=255) aaTAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGc > 2:4839140/1‑76 (MQ=255) aaTAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGc < 2:1885017/76‑1 (MQ=255) aTAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCa > 1:4042782/1‑76 (MQ=255) tAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCAt > 1:210439/1‑76 (MQ=255) cccGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTaa > 2:4217907/1‑76 (MQ=255) cccGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTaa < 2:1722703/76‑1 (MQ=255) cccGTTTGCGCGTTTGGACGAGCTCTTCGTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTaa < 2:4342284/76‑1 (MQ=255) ccGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAAc < 2:1528335/76‑1 (MQ=255) ccGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAAc < 1:4547315/76‑1 (MQ=255) cGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAAcc > 1:1668912/1‑76 (MQ=255) cGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAAcc < 2:4656781/76‑1 (MQ=255) tttGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCt < 1:2671411/76‑1 (MQ=255) ttGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCtt > 2:4526916/1‑76 (MQ=255) ttGCGCGTTTGGGCGAGCTCTTCTTTGAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCtt < 2:1300165/76‑1 (MQ=255) tGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCttt < 2:300874/76‑1 (MQ=255) tGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCttt > 1:2179880/1‑76 (MQ=255) cgcgTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGc > 1:4704828/1‑76 (MQ=255) cgcgTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGCATAGGCATGGAACACTTTGc > 1:3765794/1‑76 (MQ=255) gcgTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCg > 1:1880842/1‑76 (MQ=255) gcgTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCg < 2:1937819/76‑1 (MQ=255) cgTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCgg > 1:507295/1‑76 (MQ=255) gTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGt < 1:2994603/76‑1 (MQ=255) gTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGt > 1:3195924/1‑76 (MQ=255) tttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTg < 2:3946642/76‑1 (MQ=255) ttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgc < 1:3413187/76‑1 (MQ=255) ttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgc < 2:4410526/76‑1 (MQ=255) ttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgc > 1:2487719/1‑76 (MQ=255) ttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgc > 2:1314482/1‑76 (MQ=255) ttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgc > 1:1159122/1‑76 (MQ=255) gggCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGTTgcag > 2:4982090/1‑76 (MQ=255) gggCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgcag > 1:3602750/1‑76 (MQ=255) gggCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgcag > 2:3616512/1‑76 (MQ=255) gggCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgcag > 1:3386610/1‑76 (MQ=255) gggCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgcag > 2:4181853/1‑76 (MQ=255) cGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGcagcag > 2:3424684/1‑76 (MQ=255) cGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGcagcag > 1:3529525/1‑76 (MQ=255) | CTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAG > REL606/4201887‑4202031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |