| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,396,897 | A→T | I295N (ATC→AAC) | yjfR ← | predicted L‑ascorbate 6‑phosphate lactonase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,396,897 | 0 | A | T | 98.7% | 586.6 / 0.1 | 149 | I295N (ATC→AAC) | yjfR | predicted L‑ascorbate 6‑phosphate lactonase |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base T (54/93); total (54/95) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01 | |||||||||||
Only 100 of 152 total aligned reads displayed.
CCGCCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTCAGG > REL606/4396825‑4396968 | cgcccAACCTGCCAGATAAACGGCTCGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTtct < 2:2044977/73‑1 (MQ=255)ccgccACCCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTtct < 1:1596178/76‑1 (MQ=255)ccgccAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTtct > 2:3053043/1‑76 (MQ=255)cccccAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTtct < 2:51700/73‑1 (MQ=255) cgccAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTtctc > 1:4287505/1‑76 (MQ=255) gccAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTctct > 1:3760728/1‑76 (MQ=255) ccAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCtt > 1:4948424/1‑76 (MQ=255) cAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTg > 2:408509/1‑76 (MQ=255) cAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTg < 2:2631408/76‑1 (MQ=255) aCCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCg < 2:1922881/76‑1 (MQ=255) aCCTGCCAGAAAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCg < 1:860816/76‑1 (MQ=255) tGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGAt > 2:4495735/1‑76 (MQ=255) gCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATc > 1:1228762/1‑76 (MQ=255) ccAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCg < 1:1400413/76‑1 (MQ=255) ccAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCg < 1:4212544/76‑1 (MQ=255) ccAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCg < 2:2614418/76‑1 (MQ=255) ccAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCg < 1:3212366/76‑1 (MQ=255) ccAGATAAACGGCTTGAAGCCATAATTCAGGAGATGGTTTTTCATTTCCCCCAGCACGCGGTTCTCTTGCGGGTCg > 2:1504141/1‑76 (MQ=255) cAGAAAAAGGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCgg < 2:4731692/76‑1 (MQ=255) aGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGc > 1:2833423/1‑76 (MQ=255) aGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCCGc > 2:4329923/1‑76 (MQ=255) aGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCCGc > 2:362975/1‑76 (MQ=255) aTAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCt > 1:4367148/1‑76 (MQ=255) tAAGCGGCTTAAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTg < 2:4543423/76‑1 (MQ=255) tAAACGGTTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTg < 2:1644591/76‑1 (MQ=255) tAAACGGCTGGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTg < 2:3006006/76‑1 (MQ=255) aaaaCGGCTTAAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTg < 2:255723/75‑1 (MQ=255) aaaCGTTTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTgg < 2:1934828/76‑1 (MQ=255) aaaCGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTgg > 1:1552219/1‑76 (MQ=255) ccGGCTTAAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGaa < 2:4218559/75‑1 (MQ=255) aCGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGaa < 1:2960588/76‑1 (MQ=255) cGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAg < 1:3462426/76‑1 (MQ=255) cGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAg < 1:1399829/76‑1 (MQ=255) gCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGtt > 1:112981/1‑76 (MQ=255) gCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGtt < 1:2971206/76‑1 (MQ=255) ttGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTg < 2:4883984/76‑1 (MQ=255) tGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGa < 2:3650325/76‑1 (MQ=255) tGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGa > 1:1545845/1‑76 (MQ=255) gAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGAc > 2:1577746/1‑76 (MQ=255) aaGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGAcc < 1:1093281/76‑1 (MQ=255) gCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAg < 2:1732071/76‑1 (MQ=255) gCCATACTACAGGCGATCTTTTTTCATCTCCCACAGCTCGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAg < 2:5023093/76‑1 (MQ=255) ccATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGa > 1:5011130/1‑76 (MQ=255) cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGat > 1:1838689/1‑76 (MQ=255) cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGat < 1:4620297/76‑1 (MQ=255) cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGat > 1:2609439/1‑76 (MQ=255) cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGat < 1:3438398/76‑1 (MQ=255) cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGat < 2:1218918/76‑1 (MQ=255) aTACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGata < 2:3836161/76‑1 (MQ=255) aTACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGata < 2:225078/76‑1 (MQ=255) aTACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGata > 1:616807/1‑76 (MQ=255) aCTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATc < 1:2206171/76‑1 (MQ=255) tCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtg < 2:4126690/76‑1 (MQ=255) cAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtgg < 1:877144/76‑1 (MQ=255) cAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtgg > 2:2393266/1‑76 (MQ=255) gCGATCTTTTTTCGTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGtggtgg < 1:682995/76‑1 (MQ=255) gCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGtggtgg < 2:4156010/76‑1 (MQ=255) cGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGa < 2:1503093/76‑1 (MQ=255) cGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGa > 2:4951952/1‑76 (MQ=255) cGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGa < 1:1670898/76‑1 (MQ=255) cGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGGGGa > 2:101707/1‑76 (MQ=255) aTCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAAc > 1:4839639/1‑76 (MQ=255) tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 1:4279649/76‑1 (MQ=255) tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 2:4234361/76‑1 (MQ=255) tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 1:2053284/76‑1 (MQ=255) tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg > 2:3212193/1‑76 (MQ=255) tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 2:210912/76‑1 (MQ=255) tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 1:4238723/76‑1 (MQ=255) tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 2:1404413/76‑1 (MQ=255) ttttttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACggg < 2:2696313/76‑1 (MQ=255) ttttttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACggg < 1:2659841/76‑1 (MQ=255) ttttttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGGGGAACggg > 2:3200160/1‑76 (MQ=255) tttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATa < 1:2495106/76‑1 (MQ=255) tttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATa < 1:2213216/76‑1 (MQ=255) tttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATa < 1:4760071/76‑1 (MQ=255) tttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATa > 1:4990749/1‑76 (MQ=255) ttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATaa < 1:1822447/76‑1 (MQ=255) ttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATaa > 1:3664451/1‑76 (MQ=255) tCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCCGATAACGTGGTGGAACGGGATAAc > 2:4382046/1‑76 (MQ=255) tCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAAc < 2:2075456/76‑1 (MQ=255) tCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAAc > 1:3375320/1‑76 (MQ=255) cATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAAcc < 1:4482219/76‑1 (MQ=255) aTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCa < 2:3890328/76‑1 (MQ=255) aTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCa < 2:2582498/76‑1 (MQ=255) aTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCa < 2:4152902/76‑1 (MQ=255) aTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCa < 2:114544/76‑1 (MQ=255) aTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCa < 2:737896/76‑1 (MQ=255) cccACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAAACACttt > 2:1338961/1‑76 (MQ=255) ccACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTc < 1:3267604/76‑1 (MQ=255) ccACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTc > 2:727825/1‑76 (MQ=255) aGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCAt < 2:2836372/76‑1 (MQ=255) aGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCAt < 2:1790292/76‑1 (MQ=255) aGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCAt < 1:2321595/76‑1 (MQ=255) aGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCAt < 1:1258312/76‑1 (MQ=255) gCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCAtt < 2:921342/76‑1 (MQ=255) cACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTc > 2:4302549/1‑76 (MQ=255) aCGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTCa > 1:2898818/1‑76 (MQ=255) gcgGTTCTCTTGCGGATCGGCCTGTAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTCAgg < 2:302187/76‑1 (MQ=255) gcgGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTCAgg < 1:2116239/76‑1 (MQ=255) gcgGTTCTCTCGCGGATCGGCCTGGAAATTTTAGCGGCTATCGTGGTGGGACGGGATAACCCCTTTAGGATTCacg > 2:2432612/1‑74 (MQ=255) | CCGCCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTCAGG > REL606/4396825‑4396968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |