BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	931,164	T→G	E134A (GAA→GCA)	ybjE ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	931,164	0	T	G	100.0%	651.0 / NA	152	E134A (GAA→GCA)	ybjE	predicted transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (85/67); total (85/67)

Only 100 of 153 total aligned reads displayed.

TAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGCACAGTT  >  REL606/931091‑931236

tAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCg                                                                        >  1:717126/1‑76 (MQ=255)

 aaGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCgg                                                                       >  2:2698002/1‑76 (MQ=255)

 aaGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCgg                                                                       >  1:2994345/1‑76 (MQ=255)

 aaGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCgg                                                                       <  1:3558850/76‑1 (MQ=255)

    gTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTcg                                                                    <  1:3364452/76‑1 (MQ=255)

    gTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTcg                                                                    <  1:921895/76‑1 (MQ=255)

    gTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTcg                                                                    <  1:768178/76‑1 (MQ=255)

    gTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTcg                                                                    <  1:578365/76‑1 (MQ=255)

       aTGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCgt                                                                 <  2:1610023/76‑1 (MQ=255)

         gCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCgtgt                                                               >  2:3090408/1‑76 (MQ=255)

         gCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCgtgt                                                               >  1:4777370/1‑76 (MQ=255)

         gCCATTATTGCGCAACTAAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCgtgt                                                               >  1:4177098/1‑76 (MQ=255)

          ccATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGtgtt                                                              <  2:4054665/76‑1 (MQ=255)

           cATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGtgttg                                                             >  1:1942471/1‑76 (MQ=255)

           cATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGtgttg                                                             <  1:962079/76‑1 (MQ=255)

           cATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGtgttg                                                             >  1:233323/1‑76 (MQ=255)

           cATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGGCGCGtgttg                                                             >  2:4089875/1‑76 (MQ=255)

            attattGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGtgttgt                                                            <  2:4833555/76‑1 (MQ=255)

            attattGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGtgttgt                                                            >  1:4814594/1‑76 (MQ=255)

            attattGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGtgttgt                                                            <  2:169965/76‑1 (MQ=255)

             ttattGCTCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTa                                                           <  1:908211/76‑1 (MQ=255)

             ttattGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTa                                                           <  2:3355525/76‑1 (MQ=255)

             ttattGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTa                                                           <  2:644160/76‑1 (MQ=255)

              tattGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTaa                                                          >  2:1376014/1‑76 (MQ=255)

               attGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAg                                                         <  1:3143327/76‑1 (MQ=255)

                 tGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGaa                                                       >  1:3029402/1‑76 (MQ=255)

                  gcgcAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGaaa                                                      <  2:4755947/76‑1 (MQ=255)

                  gcgcAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGGGTTGTAAGaaa                                                      >  1:3274973/1‑76 (MQ=255)

                   cgcAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAg                                                     <  2:3588309/76‑1 (MQ=255)

                     cAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGcc                                                   >  1:2014718/1‑76 (MQ=255)

                      aaCTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCa                                                  >  1:302955/1‑76 (MQ=255)

                      aaCTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCa                                                  >  1:3176375/1‑76 (MQ=255)

                      aaCTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCa                                                  <  1:2210252/76‑1 (MQ=255)

                      aaCTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCa                                                  >  1:4349710/1‑76 (MQ=255)

                       aCTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAg                                                 >  1:3322720/1‑76 (MQ=255)

                        cTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGGGGTGTAAGAAAGCCAGt                                                >  2:4566631/1‑76 (MQ=255)

                         tGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTc                                               >  2:4554802/1‑76 (MQ=255)

                         tGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTc                                               >  2:511636/1‑76 (MQ=255)

                          gAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTcc                                              >  2:3208038/1‑76 (MQ=255)

                           aaTACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCa                                             >  2:4449161/1‑76 (MQ=255)

                           aaTACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCa                                             >  2:2191531/1‑76 (MQ=255)

                             tACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACt                                           <  1:4987273/76‑1 (MQ=255)

                              aCCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTc                                          >  1:2540124/1‑76 (MQ=255)

                              aCCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTc                                          >  1:1695443/1‑76 (MQ=255)

                              aCCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTc                                          <  2:3189331/76‑1 (MQ=255)

                               ccAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCa                                         <  1:2111181/76‑1 (MQ=255)

                                cAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAg                                        <  1:2849133/76‑1 (MQ=255)

                                   cGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGAcc                                     >  2:356549/1‑76 (MQ=255)

                                    gAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCa                                    <  1:4501573/76‑1 (MQ=255)

                                     aGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGTCAGTCCACTCAGACCaa                                   >  2:2504041/1‑76 (MQ=255)

                                     aGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCaa                                   >  1:2063910/1‑76 (MQ=255)

                                      ggAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAAt                                  >  2:1451205/1‑76 (MQ=255)

                                       gAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATg                                 >  2:4828199/1‑76 (MQ=255)

                                       gAAAAGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATg                                 <  2:2925835/76‑1 (MQ=255)

                                        aaaaGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATgg                                <  2:2800525/76‑1 (MQ=255)

                                           aGTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCaa                             >  1:2331113/1‑76 (MQ=255)

                                            gTAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCaaa                            <  1:3104769/76‑1 (MQ=255)

                                             tAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCaaaa                           <  2:2890781/76‑1 (MQ=255)

                                             tAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCaaaa                           >  1:4932085/1‑76 (MQ=255)

                                             tAGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTATTGTAAGAAAGCCAGTCCACTCAGACCAATGGCaaaa                           >  1:2588477/1‑76 (MQ=255)

                                              aGCAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAAc                          >  2:3674647/1‑76 (MQ=255)

                                                cAAAATTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCa                        <  2:3862961/76‑1 (MQ=255)

                                                 aaaaTTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCaa                       <  1:4578888/76‑1 (MQ=255)

                                                 aaaaTTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCaa                       >  1:3719335/1‑76 (MQ=255)

                                                 aaaaTTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGAAAAACCaa                       >  2:2009322/1‑76 (MQ=255)

                                                  aaaTTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAAt                      <  1:1599802/76‑1 (MQ=255)

                                                   aaTTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATTGCAAAACCAATc                     >  1:400152/1‑76 (MQ=255)

                                                   aaTTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATc                     <  1:2974336/76‑1 (MQ=255)

                                                   aaTTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATc                     <  1:2679759/76‑1 (MQ=255)

                                                   aaTTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATc                     >  2:2122964/1‑76 (MQ=255)

                                                   aaTTAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAAACAATc                     >  2:4699026/1‑76 (MQ=255)

                                                   aaTTAACGTGTATTCACTGGCTGCGGTCGCGGGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAAACAATc                     >  2:171159/1‑76 (MQ=255)

                                                     ttAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCac                   >  1:697200/1‑76 (MQ=255)

                                                      tAACGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCact                  >  1:1108607/1‑76 (MQ=255)

                                                         cGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCactact               >  1:1823616/1‑76 (MQ=255)

                                                         cGTGTATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCactact               <  2:1461993/76‑1 (MQ=255)

                                                          gtgtATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCActacta              <  1:2316614/76‑1 (MQ=255)

                                                           tgtATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACtactac             >  2:3884244/1‑76 (MQ=255)

                                                           tgtATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACtactac             <  2:1238232/76‑1 (MQ=255)

                                                            gtATTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTAcg            <  1:974332/76‑1 (MQ=255)

                                                              aTTCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTAcgcc          >  1:2190972/1‑76 (MQ=255)

                                                               ttCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTAcgccg         >  1:108559/1‑76 (MQ=255)

                                                               ttCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTAcgccg         >  1:3387392/1‑76 (MQ=255)

                                                               ttCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTAcgccg         >  2:1500394/1‑76 (MQ=255)

                                                                tCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTAcgccgc        <  1:253254/76‑1 (MQ=255)

                                                                tCACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTAcgccgc        >  1:1912821/1‑76 (MQ=255)

                                                                 cACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTAcgccgcc       >  2:3840639/1‑75 (MQ=255)

                                                                 cACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGca       <  1:596070/76‑1 (MQ=255)

                                                                 cACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGca       <  2:3367002/76‑1 (MQ=255)

                                                                 cACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGca       >  1:1571173/1‑76 (MQ=255)

                                                                 cACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGca       <  1:27135/76‑1 (MQ=255)

                                                                 cACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGca       >  2:1959798/1‑76 (MQ=255)

                                                                 cACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGca       <  1:2113619/76‑1 (MQ=255)

                                                                 cACTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACCCAGACCAATGGCAAAACCAATAACTACTACGCCGca       >  2:2098663/1‑76 (MQ=255)

                                                                  aCTGGCTGCGGGCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGcac      >  2:3704996/1‑76 (MQ=255)

                                                                  aCTGGCTGCGGGCGCGGGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGcac      >  2:4419416/1‑76 (MQ=255)

                                                                   cTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGcaca     >  2:2581136/1‑76 (MQ=255)

                                                                   cTGGCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGcaca     >  1:4262801/1‑76 (MQ=255)

                                                                     ggCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGCACAGt   >  2:926415/1‑76 (MQ=255)

                                                                      gCTGCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGCACAGtt  >  2:3041799/1‑76 (MQ=255)

TAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTCAGACCAATGGCAAAACCAATCACTACTACGCCGCACAGTT  >  REL606/931091‑931236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`