| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,131,458 | 0 | A | . | 96.9% | 460.3 / 8.3 | 96 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base . (74/19); total (76/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 164 total aligned reads displayed.
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533 | aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 2:6976070/1‑76 (MQ=255)aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa > 1:6787798/1‑76 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 2:2030107/76‑1 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 1:1141939/1‑76 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 1:1157195/76‑1 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 2:3317273/1‑76 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 1:4385161/76‑1 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 1:5751335/1‑76 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 2:4285148/76‑1 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa > 2:6112257/1‑76 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 2:1092528/76‑1 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa > 2:5216074/1‑76 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa > 2:5295018/1‑76 (MQ=255) tgaTGTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 1:4851028/76‑1 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa < 2:883753/76‑1 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa < 1:1101173/76‑1 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa < 1:1134604/76‑1 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa < 1:4697142/76‑1 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 2:220319/1‑76 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa < 1:1877397/76‑1 (MQ=255) ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa < 1:2925093/76‑1 (MQ=255) ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa > 2:6901992/1‑76 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 2:1286577/1‑75 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac < 1:4003487/76‑2 (MQ=255) tGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaac > 1:4559389/1‑75 (MQ=255) gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 1:6063720/1‑74 (MQ=255) tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg < 2:3607867/76‑4 (MQ=255) tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg < 2:92561/76‑4 (MQ=255) tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg > 1:2429409/1‑73 (MQ=255) tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaaagg > 2:7007304/1‑74 (MQ=255) tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 2:3693565/1‑76 (MQ=255) tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc > 1:6313548/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:4261592/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:6904131/1‑76 (MQ=255) tAAATGTCGAGATAACGATCACAAAAACTACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc < 2:1602833/76‑1 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:1394965/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑‑AAAAAAACGGCCTccc > 1:2774384/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAACAACGACAATATGAAAATAAATAGAGGAATGAAAGGC‑AAAATAAACGGCCTccc > 2:5706496/1‑76 (MQ=255) tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑gg < 2:3337015/76‑1 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa > 1:5800287/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGa > 1:2919038/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATTAAAAGTAATAGAGGAATGAAATGC‑AAAAAAAACGGCCTCCC‑GGaat < 2:6625749/76‑3 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 1:4303328/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 2:5381666/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAgg > 2:6294818/1‑76 (MQ=255) agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGa < 2:5598392/76‑1 (MQ=255) gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGaa < 1:2697642/76‑1 (MQ=255) gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGaa < 1:3246484/76‑1 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGc < 1:2091397/76‑1 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGcc < 1:1482421/76‑1 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGcc > 2:1046499/1‑76 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCg > 1:5644379/1‑76 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCg < 1:4896633/76‑1 (MQ=255) cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGc > 1:6727708/1‑76 (MQ=255) cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGc > 1:7070589/1‑76 (MQ=255) gATCACAAAAACGACAATAGGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGca < 1:6625256/76‑1 (MQ=255) cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGcaaca > 2:1924223/1‑76 (MQ=255) aaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAg > 1:37805/1‑76 (MQ=255) cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTgcgc > 2:1762320/1‑76 (MQ=255) cGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTgcgc > 1:3523303/1‑76 (MQ=255) gACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGcgcg > 2:4701675/1‑76 (MQ=255) aCAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGt > 2:5697836/1‑76 (MQ=255) atGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGttt > 2:5127939/1‑76 (MQ=255) atGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGttt > 1:5624516/1‑76 (MQ=255) gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 1:6211296/1‑76 (MQ=255) gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa > 1:5523169/1‑76 (MQ=255) aaaaaTTAATAGAGAAAGGAGATG‑AAAAAAGACCGGCCGCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa < 1:5492563/75‑1 (MQ=255) aaaaTAAAAGAGGAAATAAAGGGAAAAAAAACCGCCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:2984640/76‑1 (MQ=255) aTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa > 1:2955812/1‑76 (MQ=255) ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg > 2:1653160/1‑76 (MQ=255) tAATAGAGGAATGAAAGGCAAAAAAAAA‑GGCCTCCC‑GGGGGGAAGCCGGCACAAAGGGCGCGTGGGTTTATGCAgg > 2:443703/1‑76 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:5981022/1‑76 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 2:3192618/1‑76 (MQ=255) tAATAGAGGAATGAAAGG‑AAAAAAAACCGGCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg < 2:2762632/76‑1 (MQ=255) aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 1:4118083/1‑76 (MQ=255) agggagGAAAGAAA‑GAAAAAAAAAACGCCCTCCC‑GGAGGTAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 2:248305/73‑1 (MQ=255) aTAGATGAAT‑AAAAGAAAAAAAAAACGGCCCCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 1:4154897/76‑1 (MQ=255) aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt > 1:2023957/1‑76 (MQ=255) tAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATg > 1:6019716/1‑76 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc > 2:5584051/1‑76 (MQ=255) agGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg > 2:3451244/1‑76 (MQ=255) agGAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcgg > 2:4082163/1‑76 (MQ=255) ggAATGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggc > 1:1383984/1‑76 (MQ=255) aaagAAAGG‑AAAAAAAAACGGCCCCCG‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt < 1:842026/73‑1 (MQ=38) aaTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 1:4937647/1‑76 (MQ=255) aaTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 2:3104908/1‑76 (MQ=255) aTGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa > 1:6988122/1‑76 (MQ=255) ttaaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAACCGCAACCAAGTGCGCGGGGGTTTATGCAGGATGCGGCGTaa > 1:3216147/3‑76 (MQ=255) tGAAAGGC‑AAAAAAACCGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa < 1:4873019/76‑1 (MQ=38) tGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 2:2128139/1‑76 (MQ=255) tGAAAGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 2:4730778/1‑76 (MQ=255) gaaGGCAAAAAAAAACGGCCCACC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaaa < 1:504651/75‑1 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:2241975/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:2946137/1‑76 (MQ=255) aaaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc > 2:2452946/1‑76 (MQ=255) aaGGC‑AAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACa > 2:1996119/1‑76 (MQ=255) aGGGAAAAAAAACCGGCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACa < 1:3562744/76‑1 (MQ=37) agtaaaaaaaacCGGCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACat < 1:4651533/73‑1 (MQ=37) aaaaaaaacCGGCCCCCC‑GGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAtg < 1:6441705/76‑1 (MQ=38) aaaaaaaaaCCGCCCCCCGGGAGGAAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACatat < 2:3196489/76‑1 (MQ=37) | AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCC‑GGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATATG > REL606/2131383‑2131533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |