| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,376,348 | 0 | T | G | 41.7% | 238.7 / 45.6 | 156 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base T (18/73); new base G (65/0); total (83/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-27 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 208 total aligned reads displayed.
CACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGACGCGCCGCGAC > REL606/2376274‑2376423 | cACCAGATGAGGCGAACTGTAGCCGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtg < 2:4675818/76‑1 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtggt < 1:851645/76‑1 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtggg > 1:6178902/1‑75 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAgggggg > 2:244684/1‑75 (MQ=255) aGATGGGGCGAACTGAAGACGCCCCCTTTGTACCCTCCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGc < 2:3336218/76‑1 (MQ=255) aTGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGggggtggcc > 2:5982413/1‑73 (MQ=255) aTGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCt > 2:1198345/1‑76 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCtt < 2:6404392/76‑1 (MQ=255) ggAGGCAAACTGTAGACGCCCATTTGGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCtt < 2:4733519/75‑1 (MQ=255) gAGGCGAACTTTAGCCGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 2:6662081/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 1:6748637/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCttt > 1:5238581/1‑76 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCtttt > 1:4642375/1‑75 (MQ=255) ggCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGc < 1:5517226/76‑1 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCCTTTCCa > 2:4063275/1‑76 (MQ=255) aaCTGTAGCCGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCAtt < 2:6838821/76‑1 (MQ=255) aaCGGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCAtt < 2:1938820/76‑1 (MQ=255) aCTGTAGACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTc < 1:5790373/76‑1 (MQ=255) gTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTa < 1:4212276/76‑1 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTa > 1:1469457/1‑76 (MQ=255) tAGACGCCCACTTTGTCCCCTGCCGCCATCACAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAc < 1:4140022/76‑1 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAc < 1:1554005/76‑1 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCCTTGCCATTCGTAc > 2:3003347/1‑76 (MQ=255) aGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAcc < 2:3063763/76‑1 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCTTTGCCATTCCTAcc > 1:2504558/1‑76 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTAcc > 2:318719/1‑76 (MQ=255) gACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAccc < 1:4847438/76‑1 (MQ=255) cccccTCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGc < 2:5545268/74‑1 (MQ=255) gCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTTCCCGCa > 1:1617550/1‑76 (MQ=255) gCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGTCATTTGTAaccccc > 1:709033/1‑73 (MQ=255) ccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAAc < 2:7025432/76‑1 (MQ=255) cccTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACg < 1:2409171/74‑1 (MQ=255) cccTTTGTCCCCGGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACg < 2:4316872/74‑1 (MQ=255) cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCATTCGTACCCGCCACg > 1:6848355/1‑76 (MQ=255) cTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGt < 1:2834187/76‑1 (MQ=255) tttGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTa < 1:4716145/76‑1 (MQ=255) tttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCTTTGCCATTCCTATCCGCAACggga > 2:1526881/1‑74 (MQ=255) tttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTa > 1:629529/1‑76 (MQ=255) tttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTa > 1:5387274/1‑76 (MQ=255) ttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACCGTaa > 1:3323542/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTaaa > 1:1296645/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTAACCGCAACGGTaaa > 1:1991779/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCCTACCCCCAAAGGGaaa > 1:3788650/1‑76 (MQ=255) tcccccGGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAac < 2:2685145/73‑1 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAAACGTAAac > 1:4301840/1‑76 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCATTCGTACCCGCCACGGTAAac > 1:6589603/1‑76 (MQ=255) gTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAac > 1:5131946/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACCGTAAaca > 1:1426784/1‑76 (MQ=255) ccccTGCCGCCATCAGAATCGACTCCAGCGTACGGCGGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAacac < 2:2171732/75‑1 (MQ=255) ccccTCCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAacac < 2:6524392/75‑1 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTTCCATTCGTACCCGCCACGGTAAacac > 1:4462121/1‑76 (MQ=255) cccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAAcaca > 2:6936817/1‑76 (MQ=255) cccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGAGGCTTTGCCGTTCGTACCCGCCACGGTAAAcaca > 2:5321920/1‑76 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTTCCATTCGTACCCGCAACGGTAAACACaa > 1:2701756/1‑76 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCCTTGCCCTTCGTACCCGCAAAGGCAAacacca > 2:2804225/1‑74 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaa < 1:5211304/76‑1 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGGAAAAACaaa > 2:3556533/1‑76 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCTTTGCCATTCGTACCCGCAACGGTAAAACCAAATg > 1:3994824/1‑76 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTTCCATTCGTACCCGCAACGGGAAACACaaaag > 2:1015381/1‑74 (MQ=255) cgccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAAAGGTAAACACAAATGgc > 1:3091038/1‑76 (MQ=255) cgccATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAAAGGTAAACACAAAAGgc > 2:5817694/1‑76 (MQ=255) gccATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGgcg > 1:6331899/1‑76 (MQ=255) cATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAAAACAAATGGCGCt > 1:4734025/1‑76 (MQ=255) cATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCCCAACGGGAAACACAAAAGGCGCt > 2:4395581/1‑76 (MQ=255) accAGAATCGACCCCCGCGTCCTATAGGTGTTTCCTTTGCCATTCATACCCGCAACGGTAAACACAAATGGCGCTg < 2:5998565/74‑1 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTg < 2:5058413/76‑1 (MQ=255) tCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTgg < 2:5132976/76‑1 (MQ=255) aGAATCGCCTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGtt < 1:760221/76‑1 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGGCATTCGTACCCGCAACGGGAAACACAAATGGCGCTgggt > 1:6527934/1‑74 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAAGGCGCTgggt > 1:2951705/1‑74 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGGCATTCGTACCCGCAACGGTAAACACAAAAGGCGCTgggt > 2:2363678/1‑74 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCCACGGTAAACACAAATGGCGCTGGtt > 1:528672/1‑76 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTgggt > 1:2604741/1‑74 (MQ=255) aaTCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑c < 2:1753331/76‑1 (MQ=255) aTCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑Ca < 1:6382197/76‑1 (MQ=255) tCGCCTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAg < 2:2050356/76‑1 (MQ=255) cgccTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAgg < 1:6198777/73‑1 (MQ=255) cGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAgg < 2:1732765/76‑1 (MQ=255) gACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGa < 1:4333586/76‑1 (MQ=255) cTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACg < 2:6116449/76‑1 (MQ=255) cTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACg < 1:6315875/76‑1 (MQ=255) cTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACg < 1:2107830/76‑1 (MQ=255) cTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGGTT‑CAGGACg > 1:4658247/1‑76 (MQ=255) cggCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCa < 2:5477496/74‑1 (MQ=255) cAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCa < 1:4375550/76‑1 (MQ=255) aGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCaa < 1:5960274/76‑1 (MQ=255) gCGTACGGCAGGTGGTGCCTTTGACATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAg < 1:123169/76‑1 (MQ=255) cGTACGGCAGGGGGTGGCTTTTCCATTTGTACCCGCAACGGTAAACACAAAAGGCGCTGGGTT‑CAGGACGCCaaaa > 2:2097315/1‑74 (MQ=255) cGTACGGCAGGGGGGGGCCTTGTCATTCGTACCCGCAAACGGAGACAACAACGGCGCTGGTTTACGGGAGGCCaaa > 2:3977387/1‑75 (MQ=255) cGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAAAACAAAAGGCGCTGGTTT‑CAGGACGCCAAGa > 2:225599/1‑76 (MQ=255) gTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGAc > 1:3063338/1‑76 (MQ=255) gTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGAc < 1:6162859/76‑1 (MQ=255) cGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGAcgcg < 2:3374980/76‑1 (MQ=255) cAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGACGcgccg > 1:6081508/1‑76 (MQ=255) aGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGACGcgccgc > 2:6087701/1‑76 (MQ=255) aGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGACGcgccgc > 1:1104855/1‑76 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGACGCGCcgcg < 2:3600678/76‑1 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGACGCGCcgcg < 2:1333015/76‑1 (MQ=255) gtggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGACGCGCCGCGa > 2:3715466/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGACGCGCCGCGAc < 1:4028258/76‑1 (MQ=255) | CACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT‑CAGGACGCCAAGACGCGCCGCGAC > REL606/2376274‑2376423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |