| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,032,450 | 0 | T | G | 36.1% | 184.2 / 51.6 | 108 | T363P (ACC→CCC) | glnG | fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein |
| Reads supporting (aligned to +/- strand): ref base T (4/65); new base G (39/0); total (43/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.25e-25 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 132 total aligned reads displayed.
CAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATGCAGCAACTT > REL606/4032378‑4032525 | aaaaCAGTTCGCCGGGAAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGtt < 1:6469231/75‑1 (MQ=255) aaccAGTGCGCGGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGttt < 1:2079583/73‑1 (MQ=255) aaCAGTCCACCGGGCAACTCCTAAATCAACACTTCCTCCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGtttt < 2:6226371/76‑1 (MQ=255) aCAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTc < 1:6611531/76‑1 (MQ=255) cAGTTCGCGGGGCAAATCCTAAACCAACCCTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTcc < 1:4836023/76‑1 (MQ=255) aGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCa < 2:6808816/76‑1 (MQ=255) aGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCa < 1:5547134/76‑1 (MQ=255) aGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCa < 1:2527445/76‑1 (MQ=255) aGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTTCa > 2:3186830/1‑76 (MQ=255) aGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTTCa > 1:2268527/1‑76 (MQ=255) gTTCGCCGGGCAAATCCTGAATCAACCCTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAg < 2:239980/76‑1 (MQ=255) gTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAg < 2:6234458/76‑1 (MQ=255) tCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCt > 2:3716927/1‑76 (MQ=255) cGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTTCAGCTg > 2:6851024/1‑76 (MQ=255) gCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTTCAGCTgg > 2:5507469/1‑76 (MQ=255) gCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTTCAGCTgg > 1:1710050/1‑76 (MQ=255) ccGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGc < 1:5441237/76‑1 (MQ=255) cGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCCGGTGGCg > 2:4397685/1‑76 (MQ=255) ggccAAACCGTGAATCACCACTTCCTGCCGGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGt < 1:2491701/73‑1 (MQ=255) ggCAAATCCTGAATCAACTCTTCCTGCCGGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTa < 2:5130899/76‑1 (MQ=255) ggCAAATCCTGAACCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTa < 1:3191537/76‑1 (MQ=255) gCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTAc < 1:1168448/76‑1 (MQ=255) cAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACg < 2:4471503/76‑1 (MQ=255) cAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACg < 2:6107622/76‑1 (MQ=255) cAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTCCAGGCGGCGCACg > 1:4736119/1‑76 (MQ=255) cAAATCCTGAATCAACACTTCCTGCCCGGCGACCATCACCGTTAGCCAGCGGCAGGGGGTTTCCAGCTGGCGGACg > 2:5465150/1‑76 (MQ=255) aaaTCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTTCAGCTCGCGTACGt > 2:6017454/1‑76 (MQ=255) aaaTCCTGAACGACCATTCCCTGCCCGGCGTCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGt < 2:8391/76‑1 (MQ=255) aacccTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGtt < 2:4003801/73‑1 (MQ=255) aaTCCTGAATCAACCCTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGtt < 1:2127546/76‑1 (MQ=255) acccTGAAGCAACCCTTCCGGCCCGGCGCCCATCCCCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTg < 2:261751/74‑1 (MQ=255) aTCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTCCAGCTGGCGGACggtt > 2:3895034/1‑73 (MQ=255) tCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTTCAGCTGGCGTACGTTGc > 1:2064986/1‑76 (MQ=255) ccTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTTCCGCTGGGGGACGGTGcc > 1:6019805/1‑76 (MQ=255) cTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTTCAGCTGGCGGACGGTGCCt > 1:6880636/1‑76 (MQ=255) tGAATCACCCCTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTg < 1:2811187/76‑1 (MQ=255) tGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTg < 1:1527880/76‑1 (MQ=255) tGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTTTCGCTCGGGGACGTTGCCTg > 1:5832575/1‑76 (MQ=255) tGAATCAACACTCCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTg < 2:1608189/76‑1 (MQ=255) gAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTgg < 1:1057375/76‑1 (MQ=255) aaTCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGc < 2:5063365/76‑1 (MQ=255) cAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTTCAGCTGGGGTATGTTGTCCGGGCAc > 1:3765813/1‑76 (MQ=255) acacTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTTCAGCTGGCGTGCGTTGCCTCGGCAcac > 1:3559174/1‑74 (MQ=255) cacTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTTCCGGCGGGGTACATTTGCTGGGCACGcc > 1:5993281/1‑76 (MQ=255) cacTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTTCAGCTGGCGGACGGTGGCTCGGCCCGcc > 2:4642791/1‑76 (MQ=255) cacTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTTCAGCTGGCGGACGGTGCCTTGCCCCGcc > 1:5249465/1‑76 (MQ=255) tCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGAt < 2:6250112/76‑1 (MQ=255) ccTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATg > 1:2953459/1‑76 (MQ=255) ccTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTTCAGCTGGGGTACGGTGCCTCTCCCCGCCCGAta > 1:2408769/1‑75 (MQ=255) ccTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTTCAGCCGGCGTACGTTGGCTGGCCACGCCAGATg > 1:4265715/1‑76 (MQ=255) cTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCTGGCGTACGTTGGCTGGCCACCGCCGATGc > 1:4170355/1‑76 (MQ=255) tGCCCGGCGCCAATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCg < 2:573447/76‑1 (MQ=255) cccGGCGGCCATCCCCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTc < 2:5843113/76‑1 (MQ=255) cccGGCGGCCATCCCCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTc < 1:6027763/76‑1 (MQ=255) ccGGGGCCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCa < 2:1363693/76‑1 (MQ=255) ccGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCa < 1:3920882/76‑1 (MQ=255) cgccggcCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCag < 2:287619/73‑1 (MQ=255) cgccggcCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCag < 2:6517311/73‑1 (MQ=255) ggcggcCATCACCGTTAGCCAGCGGCAGGGGGTTTCCAGCTTGCGTACGGTGCCTGGCCCCGCCCGATGCGTCcga > 1:5757310/1‑76 (MQ=255) gcgccCATCGCCTTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCagag < 2:809963/76‑1 (MQ=255) cgcccATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGagc < 2:151981/73‑1 (MQ=255) ggcCATCACGGTTACCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGagca < 2:1180421/76‑1 (MQ=255) ggcCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGagca < 1:4573033/76‑1 (MQ=255) ggcCATCACCGTTAGCCAGCGGCAGGGGGTTTCCAGCTGGCGGACGTTGCCCGGGCACCCCAGATGCGGCAGagca > 2:2532857/1‑76 (MQ=255) gcCATCCCCGTTATCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGagcag < 1:6896887/76‑1 (MQ=255) gcCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGagcag < 2:1653857/76‑1 (MQ=255) cATCCCCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCt < 1:5500987/76‑1 (MQ=255) cATCACCGTTAGCCAGCGGCAGGGGTTTTCCCGCTGGCGTACGTTGCCTGGGCACGCCAGATGCGTCAGAGCAGCt > 2:2584527/1‑76 (MQ=255) aTCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCTGGCGGACGTTGCCTGGCCACCCCAGATGCGTCAGagcagcct > 1:3187131/1‑74 (MQ=255) aTCACCGTTAGCCAGCGGCAGGGGGTTTCCAGCTGGCGTACGTTGCCTGGCCCCGCCAGATGCGTCCGAGGACCtt > 2:3181186/1‑76 (MQ=255) tCACCGTTAGCCAGCGGCAGGGGTTTTTCAGCTGGCGTACCTTGCCTGGCCACGCCAGATGCGGCAGGGCAGCTT‑c > 1:4632316/1‑76 (MQ=255) tCACCGTTAGCCAGCGGCAGGGGTTTTCCAGGTGGCGGACGGTGCCTGGGCACGCCAGATGCGTCAGAGCAGCTT‑c > 1:1513318/1‑76 (MQ=255) tCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCTGGCGGACGTTGCCTGGCCACGCCAGATGGGTCAGAGCAGGTT‑c > 2:3105366/1‑76 (MQ=255) ccccGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑Cg < 2:6512549/74‑1 (MQ=255) cGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGtt < 2:4510418/76‑1 (MQ=255) cGTTAGCCAGCGGCAGGGGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCCT‑Cgggt > 1:4387814/1‑74 (MQ=255) tttAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGttt < 2:2406007/75‑1 (MQ=255) gTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGttt < 1:1976054/76‑1 (MQ=255) ttAGCCAGCGGCAGGGGTTTTCCAGCTGGCGTACGTTGCCTGGGCACGCCAGATGCGGCAGAGCAGCTT‑CGGGTTc > 1:1714497/1‑76 (MQ=255) tAGCCAGCGGCAGGGGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCCGAGCAGCTTCCGGTTTc > 2:771611/1‑76 (MQ=255) tAGCCAGCGGCAGGGGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGGTTcc > 1:3071306/1‑76 (MQ=255) tAGCCAGCGGCAGGGGGTTTCCAGCTGGCGGACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CCGTTTcc > 2:5104754/1‑76 (MQ=255) aGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCg < 2:4937917/76‑1 (MQ=255) aGCCAGCGGCAGGGGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGGGTCAGAGCCGCCT‑CGGGTTTCg > 1:6754738/1‑76 (MQ=255) gCCAGCGGCAGGGGTTTTTCAGCTGGCGTACGTTGCCTGGCCACGCCCGATGCGTCAGAGCAGCTT‑CGGGTTCCgg > 1:851243/1‑76 (MQ=255) ccAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGa < 2:6323118/76‑1 (MQ=255) ccAGCGGCAGGGGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGGTT‑CGGGTTCCGGa > 1:6977170/1‑76 (MQ=255) cAGCGCAAGGTGTTTTCCATCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGAt < 1:1082388/76‑1 (MQ=255) gCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATgc < 1:2806198/76‑1 (MQ=255) cGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATgca < 1:2204437/76‑1 (MQ=255) cGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATgca < 2:4011002/76‑1 (MQ=255) cGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATgca < 1:69972/76‑1 (MQ=255) cAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATgcagca < 1:2476563/76‑1 (MQ=255) cAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATgcagca > 2:679959/1‑76 (MQ=255) cAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATgcagca > 1:1098303/1‑76 (MQ=255) cAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATgcagca > 1:4793895/1‑76 (MQ=255) ggTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATGCAGCAAc < 1:1480422/76‑1 (MQ=255) gtgtTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATGCATCAACt < 2:3540739/76‑1 (MQ=255) gtgtTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATGCAGCAACt > 1:3754995/1‑76 (MQ=255) tgtTTCCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATGCAGCAACtt < 1:3670147/76‑1 (MQ=255) | CAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGTACGTTGCCTGGCCACGCCAGATGCGTCAGAGCAGCTT‑CGGTTTCCGGATGCAGCAACTT > REL606/4032378‑4032525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |