| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,180,249 | 0 | T | G | 40.2% | 180.4 / 35.5 | 107 | G24G (GGT→GGG) | yjaH | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (19/45); new base G (42/1); total (61/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 193 total aligned reads displayed.
GGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAG > REL606/4180174‑4180324 | aGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTgcg < 1:2321774/76‑1 (MQ=255) tGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCt < 2:1166385/76‑1 (MQ=255) aaCAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCCGt > 2:347676/1‑76 (MQ=255) aGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTtc < 2:3746943/76‑1 (MQ=255) gTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTtct < 2:761793/76‑1 (MQ=255) gTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTtct < 1:3334381/76‑1 (MQ=255) gTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTtct < 1:2668720/76‑1 (MQ=255) gTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTtct < 2:5966623/76‑1 (MQ=255) gTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGGGCTGTTtct > 1:2033031/1‑76 (MQ=255) ttttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTtctc < 2:623029/76‑1 (MQ=255) tttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGGGCTGTTtttct > 1:3062011/1‑75 (MQ=255) ttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCAc < 1:6545081/76‑1 (MQ=255) cGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGcc > 1:1717680/1‑76 (MQ=255) cGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTccc > 1:1407582/1‑76 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCt > 2:6971992/1‑76 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCt < 1:6918378/76‑1 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCt > 2:1637924/1‑76 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTg < 2:3366889/76‑1 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGGGCTGTTTTTCACTTcccg > 2:4177284/1‑74 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTg > 2:6857432/1‑76 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGt < 1:471272/76‑1 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGt < 1:1760566/76‑1 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACCGCCTGt > 2:1581064/1‑76 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTa < 1:261386/76‑1 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTa < 2:6108167/76‑1 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGGGCTGTTTTTCACTGCCTGTa > 1:3435236/1‑76 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGGGCTGTTTCTCACTTCCTGTa > 2:2166879/1‑76 (MQ=255) gggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTAg < 2:2339773/76‑1 (MQ=255) gggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTAg < 1:96184/76‑1 (MQ=255) gggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTAg < 1:3745742/76‑1 (MQ=255) ggtggtTTCACGCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTAGc < 2:203439/76‑1 (MQ=255) gtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTAGcc < 1:4170618/76‑1 (MQ=255) gtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGGGCCGGTTTTTACTGCCCGTTGcc > 1:5129521/1‑76 (MQ=255) tggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGGGGTGGTTTTCTCTTCCTTTCGccc > 1:5502194/1‑75 (MQ=255) ggtTTCCCCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTAGCcac < 1:6796486/76‑1 (MQ=255) ggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTAGCcac < 1:6170855/76‑1 (MQ=255) ggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGGcac > 1:4817563/1‑76 (MQ=255) gtTTCCCCCTTTTTGTTTTTCTGGCGTCGATCATTGATTCT‑GGCAGGTGCTCTGTTTCTCCCTGCCTTTAGCcaca < 2:1493611/76‑1 (MQ=255) ttCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGTGCTGTTTCTCACTTCCTGTAGCCACAAc > 2:2750712/1‑76 (MQ=255) ttCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAAc > 2:4794508/1‑76 (MQ=255) tccccctTTTTGTCTTTCTGGCGCCGATCTTTGATGCT‑GGCGGGTGCCTTGTTTCTCCCTGCTTTTAGCCACAAct < 2:3035055/73‑1 (MQ=255) tCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTTTCTCTGCCTGTAAGcacacat > 2:5649020/1‑73 (MQ=255) tCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCCCTGCCTGTAGCCACaaac > 1:5925626/1‑74 (MQ=255) tCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACaaat > 2:1973362/1‑74 (MQ=255) cACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGGGCTGTTTTTCACTCCGCGTAGCCCCaaacc > 1:2189435/1‑73 (MQ=255) cACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGCcaccaccc > 2:5113273/1‑74 (MQ=255) aCCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGGGCTGTTTCTCACTGCCTGTAGCCACCActct > 1:1648417/1‑76 (MQ=255) aCCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAAcact > 2:4058311/1‑76 (MQ=255) ccctTTTTGTCTTTCGGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTTTTCCTCACTGCCTGTAGCCACAACTCtt < 2:5182204/76‑1 (MQ=255) ccccTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCCGTAGGCCCAACCCtt > 1:5542023/1‑76 (MQ=255) ccccTTTTGTCTTTCGGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCCCTGCCTTTAGCCACAACTCtt < 2:4505269/76‑1 (MQ=255) cccTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTc < 1:904771/76‑1 (MQ=255) cccTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTTTCACTGCCTGTTGCCACAACTCTTc > 1:6610465/1‑76 (MQ=255) ccTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGGGCTGGTTCTCACTGCCTGTAGCCACAACTCTTcc > 2:5051239/1‑75 (MQ=255) cTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTTGCCACAACTCTTCAc > 1:2683744/1‑76 (MQ=255) cTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGGCACAACTCCTCAc > 2:862220/1‑76 (MQ=255) cTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCAc > 2:6904990/1‑76 (MQ=255) ttttGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGAGCGCTCTTTCTCCGTGCCCGTAGCCACAACTCCTCACt > 2:6172798/1‑76 (MQ=255) ttttGCCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCCCTGCCTGTAGCCACAACTCTTCACt < 1:2550720/76‑1 (MQ=255) tttGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTTCCTTTAGCCACAActcttctctt > 2:6387067/1‑76 (MQ=255) ttGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTTTCAATGCCTGTCGACACAACTCTGCAGTtc > 1:5323114/1‑76 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTtcc > 2:3025844/1‑76 (MQ=255) tCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGGGGGGTGTTTTTTACCGTCTGTAGCAACAACTCATCACTccctc > 1:1046448/1‑76 (MQ=255) cTTTCTGGCGTCGATCATTGATGCT‑GGCAGGCGCGCCGTTTTTCCCTCCCTCTAACCACAAACCTTCACAtccacc > 1:379359/1‑76 (MQ=255) tttCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACTTCCTccc > 1:5216809/1‑76 (MQ=255) tttCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCCCTGCCTGTAGCCACAACTCTTCACTTCCTccc < 1:1450492/76‑1 (MQ=255) ttCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAAATCTTCACTTCCTCCCt > 1:5352339/1‑76 (MQ=255) ttCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACCGCCCCTAGGCACAACACTTCACTTCCTCCCt > 1:3976784/1‑76 (MQ=255) ttCGGGCGTCGTTCATTGATGCG‑GGGGGGTGCGCGTTTTCTCGCGGCTTTTAGCCACAACTCTTCACTTCCTCCCt < 2:3259467/76‑1 (MQ=255) tCTGGCGTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCtt > 2:6320260/1‑76 (MQ=255) cTGGCGTCGATCATTGATGCT‑GGCAGGGGGGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCttt > 1:2978034/1‑76 (MQ=255) tGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTTTTTCTCACTGCGTGTAGCCACAACTCTTCACTTCCTCCCTTTa < 1:1224708/76‑1 (MQ=255) tGGCGTCGATCATTGATGCT‑GGCAGGGGGGCTGTTTCTCACTGCCTGTAGGCACAACTCTTCACTTCCTCCCTTTa > 1:2836598/1‑76 (MQ=255) gggCGTCGATCATTGATGCG‑GGCGGGGGCGTTTTTTCCCACTCCCTTTAGCCACAACTCTTCACTTCCTCCCTTTa < 2:2478799/75‑1 (MQ=255) tgCGTCGGTCATTGATGCTGGGCGGGGGCGTTTTTCCCCCCTCCCTTTAGCCACAACTCTTCACTTCCTCCCCTTa < 1:3527043/75‑1 (MQ=255) gTCGATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCCCAAATCTTCACTTCCTCCCTTTAcacc > 1:2495374/1‑73 (MQ=255) tCGCTCATTGATGCT‑GGGGGGTGCTCTTTTTCTCCCTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGcc < 1:6431282/76‑1 (MQ=255) tCGATCATTGATCCT‑GGCAGGTGCGCTGTTTCCCCCTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGcc < 1:5684100/76‑1 (MQ=255) cGATCATTGATGCT‑GGCAGGTGCGCTTTTTCTCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCa < 1:2266803/76‑1 (MQ=255) gATCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAg > 1:4383761/1‑76 (MQ=255) aTCATTGATGCT‑GGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTTCCGCCCGt > 2:5476339/1‑76 (MQ=255) tCATTGATGCT‑GGCAGGGGGGCTGTTTTTCACTGCCTGTAGGCAAGAATCTTCACTTCCTCCCTTTACCGCCACTg > 2:3902035/1‑76 (MQ=255) tCATTGATGCT‑GGCAGGGGGGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTg > 2:6513326/1‑76 (MQ=255) ttGATGTG‑GGCGGGTGCCTTGTTTCTCCCTGCTTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGAt < 1:1082391/76‑1 (MQ=255) gATGCT‑GGCGGGTGCGCTTTTTCTCCCTCCTTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGAttt < 1:2473790/76‑1 (MQ=255) aTGCT‑GGCAGGGGGGCTGTTTCTCACCGCCTGTAGCCACAACTCTTCCCTTCCTCCCTTTACCCCCCGTGGAtttt > 2:1854186/1‑75 (MQ=255) aTGCT‑GGCAGGGGGGCCGTTTCTCACCTCCTGTAGGCACAACTCTTCCCTTCCTCCCTTTACCGCCAGTGGATTTg > 2:559733/1‑76 (MQ=255) ggCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGaa > 1:347114/1‑76 (MQ=255) gCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAg > 1:5647058/1‑76 (MQ=255) cgggTGCGTTTTTCCCCCCTGCTGGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGa < 1:5364288/74‑1 (MQ=255) cAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGa > 1:6597141/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 1:2124728/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 1:10982/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 1:4522605/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 1:367573/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 2:1694332/1‑76 (MQ=255) ggTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAcc > 1:4440412/1‑76 (MQ=255) tGCGTTTTTTCTCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAg < 1:5068590/76‑1 (MQ=255) tGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAg > 1:4090893/1‑76 (MQ=255) tGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAg > 2:1913044/1‑76 (MQ=255) | GGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT‑GGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAG > REL606/4180174‑4180324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |