| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,392,556 | 0 | C | T | 44.2% | 126.3 / 116.5 | 154 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (53/33); new base T (0/68); total (53/101) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.46e-19 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 219 total aligned reads displayed.
AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 | cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 1:4189612/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa > 1:1601665/1‑76 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg < 2:1654813/76‑1 (MQ=255) aCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGc > 2:5186799/1‑76 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc > 2:2972688/1‑76 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 2:4061700/76‑1 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTCGCCa > 2:5987276/1‑76 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa < 1:5326332/76‑1 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGAGATCGGTTTTCCAGccc > 1:1010215/1‑75 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg < 1:3750115/76‑1 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGt > 2:596619/1‑76 (MQ=255) tcccTGTCTGATGGCCCGCTCGGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCTTTTTTTTAGCCAGTa < 2:6793020/74‑1 (MQ=255) taCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGAGATTTTCTAGCCAGTa > 2:4951189/1‑76 (MQ=255) aCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTAt > 2:646189/1‑76 (MQ=255) aCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTAt < 2:5316951/76‑1 (MQ=255) ccTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGGAtt > 1:3156590/1‑76 (MQ=255) cTGTCTGGTGGCGCCCTGTGCGAAGCGGTAAGGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTg < 2:228675/76‑1 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTg > 1:5188827/1‑76 (MQ=255) cTGTCTGATGCCGCGCTTGGCAAGGCGGTAACGCGTGAAGGGGCGGGCTGGAATAGCGTTTTTTTAGCCAGTATTg < 2:4589490/76‑1 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCGGGCTGGGATAGCGTTTTTTTAGCCAGTATTgg < 2:2534812/76‑1 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGGGCTTTTCTCACCAGTATTggg > 1:2660042/1‑75 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGt > 1:1201227/1‑76 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGGAACGCGTGAAGGTGCTGGCTGGGGTTGCGGTTTTTTAGCCAGAATTGGt > 1:3976931/1‑76 (MQ=255) tcggATGGCGCGCTCGGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTc < 1:1731640/73‑1 (MQ=255) tCTGATGGCGCGCCCTGCGAAGGGGTAGCGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTc < 2:4226897/76‑1 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCCAGCCCGTATTGGGca > 2:6230968/1‑76 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCCGTATTgggcc > 2:2330936/1‑73 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTcag < 2:5668068/76‑1 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTcag > 1:5023228/1‑76 (MQ=255) gATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTcagc > 2:3748193/1‑76 (MQ=255) gATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTcagc < 1:3441798/76‑1 (MQ=255) agggCGCGCTCTGCGAAGCGGTAACGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTcagca < 2:6364825/74‑1 (MQ=255) aTGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGAGATTTTCTAGCCAGTATTGGTcagca > 1:2997895/1‑76 (MQ=255) tGGCGCGCTCTGCGAGGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTcagcag < 1:401370/76‑1 (MQ=255) tGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTcagcag < 1:2702763/76‑1 (MQ=255) gcgcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGtt < 2:4718377/76‑1 (MQ=255) gcgcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGtt < 1:4419837/76‑1 (MQ=255) cgcgcTCCGGGAGGCGGTAACGCGTGAGGGTGGTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTa < 2:2425968/76‑1 (MQ=255) gcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAg < 2:5391857/76‑1 (MQ=255) cgcTCGGCGAGGCGGTAACGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAgg < 2:3651836/76‑1 (MQ=255) ccctctGAGAAGGGTTAGCGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAgg < 2:6699741/74‑1 (MQ=255) gcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGa < 1:3513414/76‑1 (MQ=255) gcTCTGCGAAGCGGTAACGCGTGAAGGGGCGGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGa < 1:5101166/76‑1 (MQ=255) gcTCGGCGAAGGGGTAACGCGTGAAGGTGCTGGCTGGAATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGa < 2:3176533/76‑1 (MQ=255) ctctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:1449764/76‑1 (MQ=255) ctctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGCTAGGaa > 1:2495376/1‑76 (MQ=255) ctcgGCGAGGCGGTAACGGTGGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:5533662/76‑1 (MQ=255) tctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAAc > 1:6824059/1‑76 (MQ=255) tctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGGTAGGAAc > 2:4409821/1‑76 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATGGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACg < 2:1272939/76‑1 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACg < 1:5238686/76‑1 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACg < 2:1788681/76‑1 (MQ=255) cggCGAGGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACg < 2:5951277/74‑1 (MQ=255) ggCGAAGCGGTAACGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACgg < 2:5440882/75‑1 (MQ=255) cGAAGCGGTACAGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCt < 2:7066041/76‑1 (MQ=255) cGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCt > 2:1865858/1‑76 (MQ=255) gaggCGGTAACGCGTGAGGGTGCGGGCTGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTg < 1:2312830/73‑1 (MQ=255) gAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGATGCGCGTTTCCAGGCAGTATTGGTTAGCAATTAGGAACGGCta > 2:5028120/1‑75 (MQ=255) cGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATc < 1:4332996/76‑1 (MQ=255) ggTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAAtac > 2:4119868/1‑74 (MQ=255) gTAACGCGTGAAGGTGCGGGCGGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATccc < 1:3186281/76‑1 (MQ=255) tAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCt > 2:1201165/1‑76 (MQ=255) aaCGTGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCtt < 2:6648351/76‑1 (MQ=255) aaCGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATcccct > 2:3587860/1‑74 (MQ=255) cgcgTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGa > 1:188425/1‑76 (MQ=255) gcgTGAAGGTGCGGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa < 1:5871069/76‑1 (MQ=255) gTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACt > 1:1664566/1‑76 (MQ=255) tGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTg > 1:86914/1‑76 (MQ=255) tGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTg > 1:6947923/1‑76 (MQ=255) tGAAGGTGCGGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAATGGCTGAATCCCTTGAACTg < 1:3612983/76‑1 (MQ=255) gAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgg > 1:4606827/1‑76 (MQ=255) aGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGGATCCCTTGAACTtggg > 2:3714869/1‑76 (MQ=255) tGCTGGCTGGGTTAGCGGTTTTCTAGCCAGGATTTGTCAGCAGTTAGGAAAGGCTGAATCCCTTGGAATGGtgccg > 2:3271756/1‑74 (MQ=255) tGCGGGTTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcgg < 2:5561766/76‑1 (MQ=255) gcgggctgtGATAGGTATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcggc < 2:649179/73‑1 (MQ=255) gtggctggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCt < 1:2220595/75‑1 (MQ=255) ctggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcgggt > 2:4862680/1‑74 (MQ=255) ctggcgggTATAGCTATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCt < 2:419548/76‑1 (MQ=255) ctggcgggGATAGCTTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCt < 1:4963958/76‑1 (MQ=255) tggttggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTg < 1:4823036/76‑1 (MQ=255) tggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCGGTTAGGAACGGCTGAATCCCTTGAAACGGggcgggcg > 2:5721179/1‑73 (MQ=255) tggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTg > 1:6124189/1‑76 (MQ=255) gctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATACCTTGAACTGGGGCGGCTGGc > 1:3843089/1‑76 (MQ=255) ctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgggg > 2:2382782/1‑74 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgggcg > 1:244232/1‑73 (MQ=255) gggATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGaa < 2:6008530/76‑1 (MQ=255) gaaTAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAt < 1:1225663/74‑1 (MQ=255) gATAGCTTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtg < 2:3343517/76‑1 (MQ=255) gATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGGAtg > 1:3735175/1‑76 (MQ=255) atggCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtgt < 1:6739994/73‑1 (MQ=255) aTAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtgt < 1:5071803/76‑1 (MQ=255) aGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGCAtgtgg > 1:2230772/1‑75 (MQ=255) gCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGaa < 2:4996789/76‑1 (MQ=255) gtttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc < 2:215829/74‑1 (MQ=255) aTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGAAAGTGAATcc > 2:7022986/1‑76 (MQ=255) ttttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCg > 1:6675438/1‑76 (MQ=255) ttttTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGc < 1:442740/76‑1 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGAATGTGAAACCGcccg > 2:2848702/1‑74 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGAAAGTGAATTCGCCTg > 2:5376044/1‑76 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGGAAGTGGATCCGCCTg > 1:2006103/1‑76 (MQ=255) | AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |