| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,474,971 | 0 | T | G | 35.5% | 373.4 / 29.4 | 166 | N261T (AAC→ACC) | idnT | L‑idonate and D‑gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base T (25/82); new base G (59/0); total (84/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.16e-25 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 211 total aligned reads displayed.
TCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGATAGCCA > REL606/4474896‑4475046 | cGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGtt < 1:3708021/76‑1 (MQ=255) cGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGtt < 1:2867987/76‑1 (MQ=255) gATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTa < 2:3694870/76‑1 (MQ=255) gATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTa < 1:3777336/76‑1 (MQ=255) aTGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTAc > 1:6936034/1‑76 (MQ=255) aTGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTAc > 1:6439015/1‑76 (MQ=255) tGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTAcc > 1:3406197/1‑76 (MQ=255) ggTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCg < 2:1834892/76‑1 (MQ=255) gCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACa > 1:4872141/1‑76 (MQ=255) cGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGAcca > 2:5920806/1‑74 (MQ=255) cGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACaa > 1:6712701/1‑76 (MQ=255) gTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGTTTACCGACaaa < 2:3819901/76‑1 (MQ=255) tCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGGACCGACaaaa > 2:52982/1‑75 (MQ=255) cATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTc < 1:4313477/76‑1 (MQ=255) cATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAACTTc > 1:7064607/1‑76 (MQ=255) aTTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCa < 1:4544847/76‑1 (MQ=255) ttACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCaa < 1:350803/76‑1 (MQ=255) ttACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTAACGCCAAAATCaa > 2:2080777/1‑76 (MQ=255) tACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTAACGAACAAATCaaa > 1:2322606/1‑76 (MQ=255) gTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAaagaa > 2:1024991/1‑76 (MQ=255) ggCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAagag < 1:4403561/76‑1 (MQ=255) ggCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAagag > 2:4912377/1‑76 (MQ=255) gCCCAGTGTGAAAACCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAgg < 1:6009923/76‑1 (MQ=255) ccAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGgcg > 2:6092240/1‑76 (MQ=255) cAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGgcgc < 2:779501/76‑1 (MQ=255) cAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGgcgc > 2:4354468/1‑76 (MQ=255) cAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGgcgc > 1:4384704/1‑76 (MQ=255) aGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCa < 1:699974/76‑1 (MQ=255) aGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCa > 1:5825107/1‑76 (MQ=255) gtgAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACgg < 1:3452165/76‑1 (MQ=255) gtgAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACgg > 1:4038753/1‑76 (MQ=255) gAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAACTCAAAGAAGAGGCGCCCggag > 1:6474977/1‑74 (MQ=255) gAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACgggg > 1:4996448/1‑74 (MQ=255) aaaaTCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGgtgt < 2:4159186/76‑1 (MQ=255) aaaTCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGtt < 1:1248937/76‑1 (MQ=255) aaaTCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGtt < 1:6246819/76‑1 (MQ=255) aaaTCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGtt < 1:5210589/76‑1 (MQ=255) aaaTCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGtt < 1:3222506/76‑1 (MQ=255) aaTCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTa < 1:3614364/76‑1 (MQ=255) aTCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAg < 2:3537775/76‑1 (MQ=255) aTCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAg < 1:4628827/76‑1 (MQ=255) tCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGt < 1:2243423/76‑1 (MQ=255) cGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGGCAAAATCAAAGAAGAGGCGCACGGTGTTAGtt > 2:331816/1‑76 (MQ=255) cGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGtt < 2:6546268/76‑1 (MQ=255) cAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGtttt < 2:1402349/76‑1 (MQ=255) cAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGtttt < 1:1348675/76‑1 (MQ=255) cAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGtttt < 2:72120/76‑1 (MQ=255) cAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGtttt > 1:6038790/1‑76 (MQ=255) cAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGtttt < 2:3211060/76‑1 (MQ=255) cAATAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGtttt > 2:1217729/1‑76 (MQ=255) aaTAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTc < 1:2346880/76‑1 (MQ=255) aaTAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTc > 2:2530254/1‑76 (MQ=255) aTAACAATGGCAATAAACAGCGCAACGGCAGGGCTACCGACAACATCCAAGAAGAGGCGCACGGGGTTAGCTTTCg > 2:2312222/1‑76 (MQ=255) tAACAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCgg > 1:5045394/1‑76 (MQ=255) aaCAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGt > 2:4029383/1‑76 (MQ=255) aCAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTa < 1:1171150/76‑1 (MQ=255) cAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTaa < 1:4232349/76‑1 (MQ=255) cAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTaa > 1:1723579/1‑76 (MQ=255) cAATGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGGaa > 1:1905951/1‑76 (MQ=255) aaTGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAAc < 1:5584817/76‑1 (MQ=255) aTGGCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACg > 2:6118953/1‑76 (MQ=255) tGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCCAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGt > 1:5229599/1‑76 (MQ=255) ggCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGGGTTAGTTTTCGGTAAAGTa > 1:2771759/1‑76 (MQ=255) ggCAATAAACAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGGGTTAGGTTTCGGTAACGTa > 1:1549550/1‑76 (MQ=255) gCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTaa < 1:2510881/76‑1 (MQ=255) gCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTaa < 2:5908776/76‑1 (MQ=255) cAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAAt > 2:142081/1‑76 (MQ=255) aTAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAAttt < 1:2620545/76‑1 (MQ=255) tAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTc > 2:5120549/1‑76 (MQ=255) tAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTc > 2:5347925/1‑76 (MQ=255) tAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGGGTTAGTTTTCGGTAAAGTAATTTc > 1:1651036/1‑76 (MQ=255) aaaCAGCGCAACGGCAGGGGTACCGACAAATTTAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTca > 2:6937067/1‑76 (MQ=255) aaaCAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTca > 1:5284032/1‑76 (MQ=255) aaCAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTcac < 1:3958380/76‑1 (MQ=255) aaCAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTcac < 2:5774788/76‑1 (MQ=255) aCAGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGGGTTAGTTTTCGGTAACGTAATTTcaca > 2:4806784/1‑76 (MQ=255) cAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACaa < 1:4144242/76‑1 (MQ=255) cAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACaa < 2:4936503/76‑1 (MQ=255) aGCGCAACGGCAGGGTTACCGACAAAATCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCCCaaa < 2:4102977/76‑1 (MQ=255) aGCGCAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACaaa > 1:1957260/1‑76 (MQ=255) cAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAAcggc < 1:3329914/76‑1 (MQ=255) cAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAAcggc < 1:2068408/76‑1 (MQ=255) cAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAAcggc < 2:4566794/76‑1 (MQ=255) cAACGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGGGTTAGTTTTTGGTAACGTAATTTCACAAAcggc > 2:1541842/1‑76 (MQ=255) cAACGGCAGGGGTACCGACAAATTCAAAAAAGAGGCGCCCGGTGGTAGGTTTCGGGAACGTCATTTTCCAAAccgc > 2:2892806/1‑76 (MQ=255) aCGGCAGGGGTACCGACAAATTCAAAGAAGAGGCGCACGGGGTTAGTTTTCGGTAACGTAATTTCACAAAcagcgg > 2:2693302/1‑76 (MQ=255) cGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAATGTAATTTCACAAACggcggc > 1:1049632/1‑76 (MQ=255) cGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACggcggc < 1:4195224/76‑1 (MQ=255) cGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACggcggc < 1:6111268/76‑1 (MQ=255) ggCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAAcggcggag > 1:1694007/1‑74 (MQ=255) ggCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGgcggcg > 2:4654480/1‑76 (MQ=255) ggCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGgcggcg < 1:2699980/76‑1 (MQ=255) cAGGTTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGAt < 2:2467673/76‑1 (MQ=255) cAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGAt < 1:6172882/76‑1 (MQ=255) aGGGTTACCGACAAATTCAAAGAAGGGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGATa > 1:6073127/1‑76 (MQ=255) aGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGATa > 2:2730935/1‑76 (MQ=255) gggTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGATAg < 2:503871/76‑1 (MQ=255) gggTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGATAg > 2:7004646/1‑76 (MQ=255) ttACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGATAGCCa > 2:5571640/1‑76 (MQ=255) ttACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGATAGCCa > 1:3133498/1‑76 (MQ=255) | TCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGATAGCCA > REL606/4474896‑4475046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |