| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 414,056 | 0 | T | G | 36.2% | 253.1 / 54.7 | 138 | T345P (ACC→CCC) | yajR | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (9/79); new base G (50/0); total (59/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-28 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 189 total aligned reads displayed.
CCCCTGACCGTCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCTTC > REL606/413982‑414131 | cccgTGCCCGTCAACCATGCCGTAAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg < 2:6054926/76‑1 (MQ=255)ccccTGCCCGAAAAACACGCCGTCAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg < 2:3544451/76‑1 (MQ=255) cccTGCCCGTCAAACATGCCGTCAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtgg < 1:1567371/76‑1 (MQ=255) cccTGACCGTCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggggg > 1:5244225/1‑76 (MQ=255) cccTGACCGTCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggggg > 2:5358217/1‑76 (MQ=255) ccTGACCGTCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtgggggg > 2:1065615/1‑75 (MQ=255) cTGACCGTCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtgggg > 1:6291116/1‑74 (MQ=255) tGACCGTCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtgggggggg > 1:3909369/1‑73 (MQ=255) tGACCGTCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAgg > 2:2546673/1‑75 (MQ=255) agACCGTCAAACAGCCCGTCAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGt < 1:3166378/75‑1 (MQ=255) aCCGTCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAggaa > 1:6831278/1‑73 (MQ=255) tCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGTAGac‑ac > 1:2893627/1‑76 (MQ=255) tCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAGac‑ac > 2:2182029/1‑76 (MQ=255) cAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGTAGAC‑Acc > 2:1097207/1‑76 (MQ=255) cAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑Acc > 1:5092519/1‑76 (MQ=255) aaaCATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAA‑Accc > 1:6880629/1‑76 (MQ=255) aaaCACGCCGTCAATCCAGCCGCCCAGCAACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑Accc < 2:5223358/76‑1 (MQ=255) accATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCa < 1:5158576/74‑1 (MQ=255) aaCATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGGGGAGAC‑Acccc > 1:2981001/1‑75 (MQ=255) aCATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGGG‑ACCCaa > 1:4007186/1‑75 (MQ=255) cATGCCGTCAACCCAGCCGCCCAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATc < 2:2764260/76‑1 (MQ=255) atcccGTCACCCCAGCCGCCGGGCAACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCg < 2:943330/73‑1 (MQ=255) gCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAGAC‑ACCCATcccc > 1:4509737/1‑76 (MQ=255) ccGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGTAGAC‑ACCCATCGCCg > 2:224584/1‑76 (MQ=255) cgccAACCCACCCCCCCAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGt < 1:1057024/73‑1 (MQ=255) gTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑ACCCCTCGccccc > 1:5592014/1‑73 (MQ=255) cAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGTAGAC‑ACCCATCGCCGG‑Acc > 1:3283378/1‑76 (MQ=255) aaTCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGTAGAC‑ACCCATCCCCGC‑ACCt > 1:2745471/1‑76 (MQ=255) aaTCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAGAC‑ACCCAACCCCGC‑ACCt > 2:3341498/1‑76 (MQ=255) aTCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑ACCCCTCGCCGG‑ACCtt > 2:2947037/1‑76 (MQ=255) cccAGCCGCCCAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCttt < 2:5142705/75‑1 (MQ=255) cccgccgccGAGCAACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTg < 2:6358137/73‑1 (MQ=255) ccAGCGTCCCAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTg < 2:1165972/76‑1 (MQ=255) ccAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGGAGAC‑ACCCCTCGCCGC‑ACCtttt > 2:6301759/1‑75 (MQ=255) ccAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACAACCCACCCCCGA‑CCCttt > 1:4329138/1‑76 (MQ=255) ccAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAA‑AACCATCGCCCT‑ACCcttt > 2:3538033/1‑75 (MQ=255) cacccgccCAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGt < 2:6407472/73‑1 (MQ=255) cAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑ACCCCCCCCCGC‑ACCTTTGt > 1:2486985/1‑76 (MQ=255) cAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGTAGAC‑ACCCATCGCCGC‑ACCctttt > 2:4838099/1‑74 (MQ=255) cAGCCCCCCAGTAACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGt < 1:6547820/76‑1 (MQ=255) aGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGTAGAC‑ACCCCACGCCGT‑AACTTTGTa > 2:1842068/1‑76 (MQ=255) aGCCCCCCAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTa < 1:6976640/76‑1 (MQ=255) cccccGGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAAcc < 2:1619453/74‑1 (MQ=255) gccCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGGGGAGAC‑ACCCATCCCCGC‑ACCTTTGTAACct > 1:132108/1‑76 (MQ=255) ccccAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACct < 2:3433535/75‑1 (MQ=255) aGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGTAGAC‑AACCATCGCCGTCACCTTT‑TAACctggtg > 2:19629/1‑76 (MQ=255) cGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTTCTGGc > 1:6731827/1‑76 (MQ=255) gacccGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCg < 1:1533051/73‑1 (MQ=255) gAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGTAGAC‑AACCACCGCCGG‑ACCTTTGTAACCTGCTGGCg > 1:6180751/1‑76 (MQ=255) gAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCCGCCGGCg > 2:6228473/1‑76 (MQ=255) gAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑AACCCTCGCCGT‑ACCTTTGTAAACTGCTGGCg > 2:4147082/1‑76 (MQ=255) acccGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGa < 1:1917511/74‑1 (MQ=255) aaCCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑ACCCATCGCCGT‑ACCTTTGTAAACTGCTGGCGa > 2:2685383/1‑76 (MQ=255) aaCCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑AACCCTAGCCGT‑ACCCTTGTTACCTTCTGGCGa > 2:2825230/1‑76 (MQ=255) ccGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGAct < 1:4670919/76‑1 (MQ=255) gCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGActct < 2:3772360/76‑1 (MQ=255) gCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGActct < 1:3791151/76‑1 (MQ=255) ccAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCtt < 1:5916902/76‑1 (MQ=255) ccAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑ACCCCGCGCCGC‑CGCCTTTTAACCTACCGGGGACCCtt > 1:874695/1‑76 (MQ=255) cAATCGCCACGCCAAGAAACTGGCTGGTGGGGGAGAA‑ACCCATCCCCGT‑TCCTTTGTTACCTGCTGGCGCCTGttt > 1:1731380/1‑76 (MQ=255) cAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCttt < 2:6786728/76‑1 (MQ=255) cAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCttt < 1:3435685/76‑1 (MQ=255) aaTCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAC‑ACCCATCCCCGT‑ACCGTTGTAACCTGCTGGCGACGCtttt > 1:5495092/1‑75 (MQ=255) aTCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTAc < 2:5090552/76‑1 (MQ=255) aTCGCCACGCCAAGAAACTGGCTGGGGGGGTAGAC‑ACCCATCGCCGT‑ACCTTTTTAAACTGCTGGCGCCTCttttc > 1:6107962/1‑74 (MQ=255) tCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACt < 1:2070993/76‑1 (MQ=255) cgccacgccaAGAAACTGGCTGGGGGAGGAGAC‑ACCCATCGCCGT‑ACCTTTGTAAACTGCTGGCGACTCTTTACTg > 1:1177430/1‑76 (MQ=255) gccacgccaAGAAACTGGCTGGGGGGGGAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTTCTGa > 2:1274504/1‑76 (MQ=255) ccacgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGAt < 2:695344/76‑1 (MQ=255) cacgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATa < 2:3276072/76‑1 (MQ=255) cacgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATa < 1:620406/76‑1 (MQ=255) cacgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATa < 1:4775397/76‑1 (MQ=255) cacgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATa < 2:1344712/76‑1 (MQ=255) cacgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATa < 2:1851757/76‑1 (MQ=255) cacgccaAGAAACTGGCTGGGGGGGGAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGAATCTTTACTGATa > 1:1074068/1‑76 (MQ=255) acgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATaa < 1:4287563/76‑1 (MQ=255) acgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATaa < 1:4949784/76‑1 (MQ=255) acgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATaa < 1:5552827/76‑1 (MQ=255) acgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATaa < 2:2565137/76‑1 (MQ=255) cgccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAg < 2:6652891/76‑1 (MQ=255) cgccaAGAAACTGGCTGGGGGAGGAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATaaa > 2:4242667/1‑75 (MQ=255) ccaAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTg < 1:1759166/76‑1 (MQ=255) ccaAGAAACTGGCTGGGGGGGGAGAC‑ACCCATCGCCGT‑ACCTTTTTAACCTGCTGGCGACCCTTTTCTTATAAATg > 2:851996/1‑76 (MQ=255) aGAAACTGGCTGGGGGGGGAGAC‑ACCCCTCGCCGT‑TCCTTTTTAACCTGCTGGCGACTCTTTTCTGATAAGTgggg > 2:3595343/1‑76 (MQ=255) aaaCTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGc < 1:6806941/76‑1 (MQ=255) aaCTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCa < 1:3633056/76‑1 (MQ=255) aCTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAg < 2:5904262/76‑1 (MQ=255) ctggctggTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTTCTGATAAGTGAGGGCAGa > 2:4516544/1‑76 (MQ=255) tggctggTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGaa < 2:3648123/76‑1 (MQ=255) tggctggTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGaa > 2:2056891/1‑76 (MQ=255) ggctggTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAg < 2:6278555/76‑1 (MQ=255) ggctggTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAg < 2:2433768/76‑1 (MQ=255) ctggTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAggg > 2:572391/1‑76 (MQ=255) ctggTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAggg < 2:5605810/76‑1 (MQ=255) tggtggAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGc > 1:1981305/1‑76 (MQ=255) ggtggAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCt < 2:3506862/76‑1 (MQ=255) ggtggAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCt > 1:4632064/1‑76 (MQ=255) gtggAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCtt > 2:6903422/1‑76 (MQ=255) tggAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCTTc < 1:6610506/76‑1 (MQ=255) tggAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCTTc < 2:3198543/76‑1 (MQ=255) | CCCCTGACCGTCAAACATGCCGTCAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGAC‑ACCCATCGCCGT‑ACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCTTC > REL606/413982‑414131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |