| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,485,450 | 0 | A | G | 40.0% | 296.5 / 167.8 | 210 | G193G (GGA→GGG) | ydcV | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (5/121); new base G (84/0); total (89/121) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 246 total aligned reads displayed.
GGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTA > REL606/1485375‑1485525 | ggttCTGGGGGCCATTGGCGGCCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCagga < 2:5835536/73‑1 (MQ=255) gATCTTGGGGCCAATGGCTGGAAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggag < 1:1142405/76‑1 (MQ=255) aTCTTGGGGCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggagg < 1:3395193/76‑1 (MQ=255) aTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGGGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggggg > 2:1494810/1‑76 (MQ=255) aTCTGGGGGCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggagg < 1:6077716/76‑1 (MQ=255) tCTGGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAggagga < 2:3225846/76‑1 (MQ=255) ttGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAt < 2:5274751/76‑1 (MQ=255) tGGGGCCAATGGCGGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATg < 2:4632082/76‑1 (MQ=255) gggCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCt < 2:262318/76‑1 (MQ=255) ggCCAGGGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTg < 2:1665399/76‑1 (MQ=255) gCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTgg < 1:6782263/76‑1 (MQ=255) gCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTgg < 2:1089803/76‑1 (MQ=255) ccAAGGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGc < 1:7020193/76‑1 (MQ=255) tggctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGttt < 2:3379844/76‑1 (MQ=255) ggcgggcAATCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTg < 2:5242103/76‑1 (MQ=255) ctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGcc < 2:1784125/76‑1 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGGTGGGGGTTGCCt > 1:7008152/1‑76 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGGCt > 2:4408595/1‑76 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGCCt > 2:2080111/1‑76 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGCTGGCGTTTGGCt > 1:3360826/1‑76 (MQ=255) ggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAGTGTGGCGTTTGccct > 1:5274566/1‑74 (MQ=255) cAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGCTGGCGTTTGCCTTGt > 2:4694791/1‑76 (MQ=255) aaaCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGGTGGGGTTTTCCTTGTc > 2:5507945/1‑76 (MQ=255) aaaCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGGTGGCGGTTGCCTTGtt > 1:1508103/1‑75 (MQ=255) aCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGGCTTGTctt > 2:572067/1‑74 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGtt < 1:5986987/76‑1 (MQ=255) ttCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCg < 1:4847173/76‑1 (MQ=255) tCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGGTGGGGGTTTGCTTgttgttttt > 1:4924320/1‑73 (MQ=255) ccGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGGTGGCGTTTGTCTTTTCGGTCgtt > 2:911170/1‑74 (MQ=255) cGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATg < 1:3613710/76‑1 (MQ=255) gCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGGGTTTGCCCTGTTGTTCGATGa > 1:4223297/1‑76 (MQ=255) gCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGa < 2:5942887/76‑1 (MQ=255) cTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGaa < 1:2405544/76‑1 (MQ=255) tACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTTTCGTTCGGTGaaa > 1:2929739/1‑76 (MQ=255) tACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTCGATGaaa > 2:2987270/1‑76 (MQ=255) tACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGCCGGCGGTTGCCCTGTTGTTTGGTGata > 1:4801357/1‑74 (MQ=255) aCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGGTGGGGGTTTCCCTGTTGGTCGGTGGAat > 1:5849627/1‑76 (MQ=255) aCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAat < 1:431254/76‑1 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAGGGTGGCGGTTGGCTTGTCGTTCGCTGAAaaca > 1:936584/1‑76 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGCTGGGGGTTGCCTTTTCGTTCGGTGAAatca > 1:5507152/1‑76 (MQ=255) tAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAAGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatcat > 1:3305190/1‑76 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTCGATGAAatcatc > 2:4512891/1‑76 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatcatc < 1:5820261/76‑1 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatcatc < 2:255144/76‑1 (MQ=255) tgtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGCTGGGGGTTGCCTTGTCGTTTGATGAAAACATCGt > 1:6186871/1‑76 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGATGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGtt < 1:1667699/76‑1 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGtt < 1:6494534/76‑1 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGtt < 2:6517901/76‑1 (MQ=255) ttGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGGGGTTGCCCTGTCGTTCGCTGAAATCATCGttt > 1:5246066/1‑75 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTTGATGAAATCATCGTTAc > 2:1184885/1‑76 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGCCTTGTCGTTCGATGAAATCATCGTTat > 1:5958326/1‑75 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTAc < 1:1063092/76‑1 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTAc < 1:4123659/76‑1 (MQ=255) gCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACg < 2:2857698/76‑1 (MQ=255) gAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGCCTTGTTGTTCGATGAAAACATCGTTACGAcc > 1:5765413/1‑76 (MQ=255) aaTCTCAGTTCGGCGTTACTGGCAGGGGGAATGCTGGCGTTTGCCTTGTCGTTTGATGAAATTATCGTTACCACCt > 2:1319567/1‑76 (MQ=255) aTCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTTTTGTTCGCTGAAAACATCGTTACTACCtt > 2:3490601/1‑76 (MQ=255) aTCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCtt < 2:6743199/76‑1 (MQ=255) tctcAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTCGGTGGAATCATCGTTAAGACCttt > 1:1016361/1‑76 (MQ=255) tctcAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCttt < 1:854577/76‑1 (MQ=255) ctcAGTTCGGCGTTACTGGCAGGGGGGATGCTGGGGTTTGCCCTGTCGTTCGCTGAAATCATCGTTACGAACtttt > 1:3207346/1‑75 (MQ=255) ctcAGTTCGGCGTTACTGGCAGGGGGAATGCTGGCGTTTGCCTTGTCGGTCGATGAAATCATCGTTACGACCTTTa > 2:6974208/1‑76 (MQ=255) tcAGTTCGGCGTTACTGGCAGGGGGAATGCTGGCGGTTGCCTTGTCGTTCGATGAAAACATCGTTAAGACCTTTAc > 1:5075541/1‑76 (MQ=255) tcAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTAc < 1:6932154/76‑1 (MQ=255) cAGTTCGGCGTTACTGGCAGGGGGGATGGTGGTGTTTTTCTTGTCGTTCTGTGAAAAAATTGTTAACGCCTTTAcc > 2:5995406/1‑75 (MQ=255) cAGTTCGGCGTTACTGGCAGGGGGGATGCTGGGGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTTCg > 1:1936852/1‑76 (MQ=255) cAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTTTCGTTCGATGAAATCATCGTTATGACCTTTAcc > 2:4986042/1‑75 (MQ=255) cAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGGCTTGTTGTTCGCTTAAATTATCGTTACGACCTTTACg > 2:1116740/1‑76 (MQ=255) cAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACg < 2:1551364/76‑1 (MQ=255) gTTCGGCGTTACTGGCAGGGGGGAAGGTGGCGGTTTCCCTGTCGTTCGGTGAAATTATTGTTAACAACTTTACGGc > 2:3838765/1‑76 (MQ=255) ttCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCCTTTAGGCa > 2:4119425/1‑76 (MQ=255) ttCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCa < 2:5092900/76‑1 (MQ=255) ttCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCa < 1:5362137/76‑1 (MQ=255) tCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTTGATGAAATCATCGTTACGACCTTTACGGCAg > 1:2683095/1‑76 (MQ=255) tCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAg < 2:6057884/76‑1 (MQ=255) cGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAgg < 2:4228693/76‑1 (MQ=255) cGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAgg < 1:3689898/76‑1 (MQ=255) ggCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGt < 1:757627/76‑1 (MQ=255) ggCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGt < 1:375010/76‑1 (MQ=255) gCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTc < 1:4036405/76‑1 (MQ=255) cGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCa < 2:1866553/76‑1 (MQ=255) gTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCAt < 2:5754152/76‑1 (MQ=255) gTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCAt < 1:6336390/76‑1 (MQ=255) gTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCAt < 2:3171238/76‑1 (MQ=255) ttACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATg < 1:6226153/76‑1 (MQ=255) tACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATga < 2:3806518/76‑1 (MQ=255) aCTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaa < 1:3495032/76‑1 (MQ=255) cTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaac < 1:6127148/76‑1 (MQ=255) cTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaac < 1:4522572/76‑1 (MQ=255) cTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaac < 2:3735925/76‑1 (MQ=255) tGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacg < 2:19011/76‑1 (MQ=255) gCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaa < 2:2414981/76‑1 (MQ=255) gCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaa < 1:134723/76‑1 (MQ=255) cAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGGATggacgcaa > 2:2820367/1‑73 (MQ=255) aggaggAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTGGTGttcgaacg > 2:88716/1‑76 (MQ=255) aggaggAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGaacgaacg < 1:3051739/76‑1 (MQ=255) aggaggAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGaacgaacg < 1:1859743/76‑1 (MQ=255) aggaggAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGGGTgtacgaaca > 1:2585742/1‑75 (MQ=255) aggaATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTa < 2:6384918/76‑1 (MQ=255) aggaATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTa < 1:325608/76‑1 (MQ=255) | GGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTA > REL606/1485375‑1485525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |