Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 1,254,938 C→T A389T (GCG→ACG)  ycgV ← predicted adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,254,9380CT96.9% 599.7 / 6.3 162A389T (GCG→ACG) ycgVpredicted adhesin
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (97/59);  total (101/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-01

Only 100 of 201 total aligned reads displayed.

CATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGCGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCC  >  REL606/1254864‑1255010
                                                                           |                                                                        
 atatCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCgtgc                                                                        >  2:1749713/1‑76 (MQ=255)
   attaaTGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCtgccgtgccg                                                                      >  2:4177728/4‑76 (MQ=255)
   atCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCtgccgtgccg                                                                      >  1:3553443/1‑76 (MQ=255)
   atCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCtgccgtgccg                                                                      >  2:5548013/1‑76 (MQ=255)
   atCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGcca                                                                      <  2:3863179/76‑2 (MQ=255)
   atCAAGGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCtgccgtgccg                                                                      <  2:6332746/76‑1 (MQ=255)
    tCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCgg                                                                     >  1:5244025/1‑76 (MQ=255)
     cAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGt                                                                    <  2:6463170/76‑1 (MQ=255)
     cAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGt                                                                    >  2:5276420/1‑76 (MQ=255)
     cAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGt                                                                    <  2:4715634/76‑1 (MQ=255)
     cAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGt                                                                    <  2:24306/76‑1 (MQ=255)
     cAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGt                                                                    >  1:2202763/1‑76 (MQ=255)
     cAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGt                                                                    <  1:6197149/76‑1 (MQ=255)
     cAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGt                                                                    <  1:5915161/76‑1 (MQ=255)
      aaTGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCgggg                                                                   >  1:417243/1‑74 (MQ=255)
      aaTGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTg                                                                   >  2:3235853/1‑76 (MQ=255)
       aTGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGa                                                                  >  1:4737696/1‑76 (MQ=255)
       aTGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGa                                                                  >  2:6518954/1‑76 (MQ=255)
        tGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GTCTCCTGCCGTGCCGGTGAt                                                                 >  2:7058126/1‑76 (MQ=255)
        tGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGAt                                                                 <  1:1173926/76‑1 (MQ=255)
        tGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGAt                                                                 >  1:925578/1‑76 (MQ=255)
         taCCAGATCGCTCGTGAGGTCGATCTTGCTGTTGGTCATGGAATAAATTCCCCG‑GGCTCCTGCGGTGCCGGTGATa                                                                <  2:3549109/75‑1 (MQ=255)
         gACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATa                                                                <  2:2977493/76‑1 (MQ=255)
         gACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATa                                                                >  1:2305063/1‑76 (MQ=255)
         gACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATa                                                                <  1:1339186/76‑1 (MQ=255)
          aCCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAg                                                               <  2:6788333/76‑1 (MQ=255)
          aCCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAg                                                               >  2:3675947/1‑76 (MQ=255)
           ccAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGc                                                              <  2:6396541/76‑1 (MQ=255)
           ccAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGc                                                              <  2:6154079/76‑1 (MQ=255)
            cAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGcc                                                             >  1:1888246/1‑76 (MQ=255)
              gttCGCGCGGGAGGCCGATCTGGCTGTTGTTCATGGCATAAATTCCCCC‑GGCTCCTGCCGTGCCGGTGATAGCCaa                                                           <  1:3799810/74‑1 (MQ=255)
               aTCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCaaa                                                          <  2:668485/76‑1 (MQ=255)
                tCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAAc                                                         <  1:6437895/76‑1 (MQ=255)
                  gCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTg                                                       >  1:5630245/1‑76 (MQ=255)
                   cTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGt                                                      >  1:6124391/1‑76 (MQ=255)
                    tCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGtc                                                     <  1:3651296/76‑1 (MQ=255)
                     cGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGtct                                                    <  2:1191384/76‑1 (MQ=255)
                     cGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGtct                                                    <  2:3338319/76‑1 (MQ=255)
                     cGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGGGATAGCCAAACTGtct                                                    >  1:4681706/1‑76 (MQ=255)
                      gTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGtctc                                                   >  1:2493450/1‑76 (MQ=255)
                      gTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGtctc                                                   >  1:3751369/1‑76 (MQ=255)
                       tGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTcc                                                  <  1:5047673/76‑1 (MQ=255)
                       tGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTcc                                                  >  1:3950399/1‑76 (MQ=255)
                        gAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCCAACTGTCTCCg                                                 >  2:6314979/1‑76 (MQ=255)
                        gAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCg                                                 >  1:1599590/1‑76 (MQ=255)
                        gAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCg                                                 <  1:4026448/76‑1 (MQ=255)
                          ggTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGGGATAGCCAAACTGTCTCCGGt                                               >  1:3706497/1‑76 (MQ=255)
                            tCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTa                                             >  2:1312824/1‑76 (MQ=255)
                            tCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTa                                             >  1:1784143/1‑76 (MQ=255)
                            tCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTa                                             <  2:408956/76‑1 (MQ=255)
                             cGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTAt                                            <  1:4177728/76‑1 (MQ=255)
                              gATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTAtt                                           <  1:2945963/76‑1 (MQ=255)
                               aTCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTc                                          <  2:228379/76‑1 (MQ=255)
                               aTCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTc                                          <  1:4810524/76‑1 (MQ=255)
                               aTCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTc                                          >  1:2036424/1‑76 (MQ=255)
                                 cTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTa                                        <  1:1461782/76‑1 (MQ=255)
                                 cTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTa                                        <  1:285414/76‑1 (MQ=255)
                                  tGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTAc                                       >  1:4337953/1‑76 (MQ=255)
                                   ggCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTAcc                                      <  2:475509/76‑1 (MQ=255)
                                    gCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTAccg                                     <  2:1623689/76‑1 (MQ=255)
                                      tGTTGGTCATGGCATAAATCCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTAccgcc                                   <  1:4130666/76‑1 (MQ=255)
                                        ttGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACcgccgc                                 >  1:4956336/1‑76 (MQ=255)
                                         tGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCt                                >  2:2487260/1‑76 (MQ=255)
                                          ggTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTg                               >  1:6040547/1‑76 (MQ=255)
                                           gTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTga                              >  1:53880/1‑76 (MQ=255)
                                           gTCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTga                              >  1:692012/1‑76 (MQ=255)
                                           gTCATGGCATAAATTCCCCT‑GGCCCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTga                              <  1:6353709/76‑1 (MQ=255)
                                            tCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTgag                             >  1:1859243/1‑76 (MQ=255)
                                            tCATGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTgag                             >  2:5844147/1‑76 (MQ=255)
                                             cATGGCATAAATCCCCCG‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTgaga                            <  1:3300768/76‑1 (MQ=255)
                                               tGGCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGc                          >  1:2645661/1‑76 (MQ=255)
                                                 gCATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGccc                        <  1:724499/76‑1 (MQ=255)
                                                  cATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCca                       >  2:2866519/1‑76 (MQ=255)
                                                  cATAAATTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCca                       >  2:4604298/1‑76 (MQ=255)
                                                   aTAAAGCCCCCG‑GCCCCCTCCCGGGCCGGTCATAGCCAAACTGTCCCCGGTTTTTTTCCCGCCGCTGAGAGCCcac                      <  2:1876254/76‑1 (MQ=255)
                                                     aaaTTCCCCT‑GGCTCCTGCCTTGCCGGTGATCGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACaa                    >  2:6670391/1‑76 (MQ=255)
                                                     aaaTCCCCCT‑GCCCCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACaa                    <  1:2005375/76‑1 (MQ=255)
                                                     aaaTCCCCCG‑CGCCCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACaa                    <  1:1162561/76‑1 (MQ=255)
                                                       aTTCCCCTGGGCCCCTGCCGTGCCGGTGATAGCCAACCTGTCTCCGGTTATTCTCCCGCCGCTGAGAGCCCACaaa                   <  2:340880/76‑1 (MQ=255)
                                                       aTTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAAc                  >  1:2375090/1‑76 (MQ=255)
                                                       aTTCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAAc                  >  2:4381617/1‑76 (MQ=255)
                                                        ttCCCCT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAAcc                 >  2:1924690/1‑76 (MQ=255)
                                                        tcccccG‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAAcc                 <  1:4464931/74‑1 (MQ=255)
                                                         tCCCCG‑GCCCCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTTTTCTCCCGCCGCTGAGAGCCCACAAAccc                <  2:2052379/76‑1 (MQ=255)
                                                          ccccT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCa               >  2:42281/1‑76 (MQ=255)
                                                          ccccT‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCa               >  2:1141200/1‑76 (MQ=255)
                                                          ccccT‑GGCCCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCa               <  1:2747367/76‑1 (MQ=255)
                                                          ccccG‑GGCTCCTGCCGTGCCGATGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCa               <  1:1635812/76‑1 (MQ=255)
                                                           cccT‑GGCCCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAg              <  1:1042631/76‑1 (MQ=255)
                                                              t‑GGCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAgcgc           >  1:4966994/1‑76 (MQ=255)
                                                                 gCTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCa         >  2:4926960/1‑76 (MQ=255)
                                                                  cTCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAg        >  1:5876431/1‑76 (MQ=255)
                                                                   tCCTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGa       >  1:3225108/1‑76 (MQ=255)
                                                                   cccTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTTTTCTCCCGCCGCTGAGAGCCCACAAACCCAGCGCCAGa       <  2:569207/75‑1 (MQ=255)
                                                                    ccTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGAc      >  2:106034/1‑76 (MQ=255)
                                                                    ccTGCCGTGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGAc      >  1:3479737/1‑76 (MQ=255)
                                                                    ccTGCCGTCCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGAc      <  1:3392574/76‑1 (MQ=255)
                                                                     ctgctgTGCCGGTGATACCAAAACTGTCTCCGGTTATTCCACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACt     <  1:5447511/76‑1 (MQ=255)
                                                                     cTGCCGCGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACt     >  2:3501865/1‑76 (MQ=255)
                                                                       gCCGTGCGGGTGATAGCAAATCTGTCTCGGTTTTTTCTCCCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGc   <  2:518113/76‑1 (MQ=255)
                                                                           |                                                                        
CATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCT‑GGCTCCTGCCGCGCCGGTGATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCC  >  REL606/1254864‑1255010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑