| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,551 | G→A | G566S (GGT→AGT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,551 | 0 | G | A | 97.6% | 1008.8 / 7.1 | 246 | G566S (GGT→AGT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base A (119/121); total (121/125) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
Only 100 of 248 total aligned reads displayed.
ACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACT > REL606/4162478‑4162622 | aCGTGCAGGCTTCGAAGTTCGAGACGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGt < 1:1876787/76‑1 (MQ=255) gTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTcc > 2:4676381/1‑76 (MQ=255) gTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTcc > 2:5433444/1‑76 (MQ=255) gCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGa < 2:2623077/76‑1 (MQ=255) gCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGa < 1:6990231/76‑1 (MQ=255) gCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAATCCGa > 2:5037957/1‑76 (MQ=255) cAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGaa > 2:7057449/1‑76 (MQ=255) ttCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGACGGTCCGAACATCg > 1:6917722/1‑76 (MQ=255) tCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCgg > 1:1196932/1‑76 (MQ=255) cGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGt < 2:3194043/76‑1 (MQ=255) cGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGt > 1:2736025/1‑76 (MQ=255) cGAAGTGCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGt < 2:4857457/76‑1 (MQ=255) gAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTc > 2:2502376/1‑76 (MQ=255) aaGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCt > 2:6186142/1‑76 (MQ=255) aaGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCt < 2:3862835/76‑1 (MQ=255) aaGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCt > 1:4495506/1‑76 (MQ=255) aaGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCt > 1:229431/1‑76 (MQ=255) ttCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACNCNTGAAAGTCCGAACATCGGTCTGAt < 1:173432/76‑1 (MQ=255) ttCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGAt < 2:4163699/76‑1 (MQ=255) cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCa > 1:3025383/1‑76 (MQ=255) cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCa > 1:2419446/1‑76 (MQ=255) gagaCGTGCCCCCGACTCACGACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCaa < 2:2731509/76‑1 (MQ=255) gagaCGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCaa < 1:647393/76‑1 (MQ=255) gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCaa < 1:2810879/76‑1 (MQ=255) gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCaa < 1:5015668/76‑1 (MQ=255) gaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAAct > 2:165147/1‑76 (MQ=255) cGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAActct < 2:5290670/76‑1 (MQ=255) cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAActct > 1:4237525/1‑76 (MQ=255) gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAActctc > 1:5537366/1‑76 (MQ=255) tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAActctct > 2:173607/1‑76 (MQ=255) ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCg > 1:518067/1‑76 (MQ=255) ccGACGCACGACGGTCGCGTATGTCCAATCGAAACCCCGGAAAGGCCCAACATCGGGCGGAGCAACGCTCTGTCCg < 1:1303802/76‑1 (MQ=255) cGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt > 2:1143219/1‑76 (MQ=255) cGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt > 1:4292848/1‑76 (MQ=255) cGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt < 2:5678738/76‑1 (MQ=255) gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtg < 2:6834012/76‑1 (MQ=255) aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt < 2:4421377/76‑1 (MQ=255) aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt < 2:3905385/76‑1 (MQ=255) cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTa > 2:5631342/1‑76 (MQ=255) cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTa > 2:3417670/1‑76 (MQ=255) cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTa > 1:1503780/1‑76 (MQ=255) tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc > 1:5907647/1‑76 (MQ=255) tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc < 2:629640/76‑1 (MQ=255) tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc > 2:3480119/1‑76 (MQ=255) cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg < 2:195718/76‑1 (MQ=255) cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg > 1:652124/1‑76 (MQ=255) cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg > 1:1536384/1‑76 (MQ=255) cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg > 1:3660433/1‑76 (MQ=255) aCTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGc > 2:1815971/1‑76 (MQ=255) cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca < 1:6239798/76‑1 (MQ=255) cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca > 2:6692774/1‑76 (MQ=255) cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca < 2:4020156/76‑1 (MQ=255) cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCCCTGTCCGTGTACGca < 2:4001861/76‑1 (MQ=255) tACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGcac > 1:3522368/1‑76 (MQ=255) cgatCGCGTATGTCCAATCGAAACCCCTGGAAGTCCGAACATCTGTCTGATCAACTCTCTGTCCGTGTACGCACAg < 2:2219513/73‑1 (MQ=255) cGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAg > 2:5113757/1‑76 (MQ=255) ggTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGa < 2:2316183/76‑1 (MQ=255) gTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc < 2:554503/76‑1 (MQ=255) gTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc < 1:107063/76‑1 (MQ=255) gTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc < 1:5046354/76‑1 (MQ=255) gTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc < 1:2633864/76‑1 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa > 1:2098011/1‑76 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa < 1:806499/76‑1 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa < 2:6531122/76‑1 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa < 2:6886163/76‑1 (MQ=255) cgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc < 1:1386018/76‑1 (MQ=255) cgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc > 2:4724047/1‑76 (MQ=255) tATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGa > 2:1358114/1‑76 (MQ=255) tATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGa > 1:4825814/1‑76 (MQ=255) aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGaa < 2:4286086/76‑1 (MQ=255) aTGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGaa > 1:4107086/1‑76 (MQ=255) tGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAAt > 1:1263384/1‑76 (MQ=255) gTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa < 1:6570290/76‑1 (MQ=255) gTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa < 2:3763186/76‑1 (MQ=255) gTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa > 2:6787945/1‑76 (MQ=255) tCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc < 2:835008/76‑1 (MQ=255) tCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc < 1:195184/76‑1 (MQ=255) ccAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 2:3672284/1‑76 (MQ=255) ccAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg < 2:6096790/76‑1 (MQ=255) cAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg < 1:6732691/76‑1 (MQ=255) cAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg < 2:6856074/76‑1 (MQ=255) cAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg < 1:3760545/76‑1 (MQ=255) tCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt < 2:2948120/76‑1 (MQ=255) tCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt < 2:1536982/76‑1 (MQ=255) cGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTc > 1:3253552/1‑76 (MQ=255) gAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTcc > 2:3371124/1‑76 (MQ=255) aaaCCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCt > 2:4984215/1‑76 (MQ=255) aaaCCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt < 2:4226790/76‑1 (MQ=255) aaCCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 2:6154468/76‑1 (MQ=255) aaCCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 2:6113203/76‑1 (MQ=255) aaCCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt > 2:779583/1‑76 (MQ=255) ccccTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCtttc > 1:5621077/1‑74 (MQ=255) cccTGAAAGGCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag < 1:3470460/76‑1 (MQ=255) ccTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTgaga > 1:6347365/1‑76 (MQ=255) cTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc < 1:4343348/76‑1 (MQ=255) cTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc > 1:2904863/1‑76 (MQ=255) tGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt < 1:5113757/76‑1 (MQ=255) tGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt < 1:4167236/76‑1 (MQ=255) tGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt < 1:6094685/76‑1 (MQ=255) tGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt > 1:6823537/1‑76 (MQ=255) | ACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACT > REL606/4162478‑4162622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |