| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,164,822 | T→C | F1323L (TTC→CTC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,164,822 | 0 | T | C | 98.7% | 959.4 / 1.1 | 224 | F1323L (TTC→CTC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (87/134); total (89/135) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01 | |||||||||||
Only 100 of 225 total aligned reads displayed.
GAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAA > REL606/4164749‑4164895 | gAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTc > 2:5383286/1‑76 (MQ=255) aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCa < 2:6769229/76‑1 (MQ=255) aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCa < 2:6653500/76‑1 (MQ=255) aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCa > 2:2072855/1‑76 (MQ=255) aaCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCa < 2:1771746/76‑1 (MQ=255) aCGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCaa < 2:2260633/76‑1 (MQ=255) gTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGt < 1:3081867/76‑1 (MQ=255) tCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTa < 2:6963760/76‑1 (MQ=255) cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAt > 2:4746119/1‑76 (MQ=255) cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAt > 1:3799041/1‑76 (MQ=255) cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAt < 1:3016223/76‑1 (MQ=255) cGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAt > 1:6170362/1‑76 (MQ=255) gTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAtt < 1:4171147/76‑1 (MQ=255) gTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAtt > 2:442460/1‑76 (MQ=255) tACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttg > 2:3166693/1‑76 (MQ=255) tACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttg < 2:3763236/76‑1 (MQ=255) aCCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgt > 2:6214419/1‑76 (MQ=255) aCCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgt < 2:3292293/76‑1 (MQ=255) aCCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgt < 2:4836814/76‑1 (MQ=255) aCCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgt > 1:2774573/1‑76 (MQ=255) ccAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgtt < 2:4465241/76‑1 (MQ=255) ccAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgtt > 1:5935965/1‑76 (MQ=255) ccAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgtt > 2:5812204/1‑76 (MQ=255) cAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgttg < 1:4476479/76‑1 (MQ=255) cAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTAttgttg > 2:3263262/1‑76 (MQ=255) aaGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGa < 2:4969920/76‑1 (MQ=255) aGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGaa < 1:5694963/76‑1 (MQ=255) gATGTATAAAAGCATCGTGGACGTCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGaaa < 2:3916517/76‑1 (MQ=255) gATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGaaa < 2:2334665/76‑1 (MQ=255) aTGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAag < 2:1593356/76‑1 (MQ=255) aTGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAag > 2:6453777/1‑76 (MQ=255) gTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAagag < 1:2582299/76‑1 (MQ=255) tataAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAgaga < 1:2230938/76‑1 (MQ=255) ataAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAt > 1:1076178/1‑76 (MQ=255) aaaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAttc < 2:5873970/76‑1 (MQ=255) aaaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttc < 1:3989820/76‑1 (MQ=255) aaaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttc > 2:6878397/1‑76 (MQ=255) aaaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttc > 1:6592956/1‑76 (MQ=255) aaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcg > 2:4204189/1‑76 (MQ=255) aaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgtt > 1:164630/1‑76 (MQ=255) aaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgtt < 1:3409965/76‑1 (MQ=255) aCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgttc > 1:44949/1‑76 (MQ=255) cATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgttcg > 1:911658/1‑76 (MQ=255) cATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGAttcgttcg < 2:3222899/76‑1 (MQ=255) tCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCt < 1:1899224/76‑1 (MQ=255) tGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTggg > 1:6705543/1‑76 (MQ=255) tGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTggg < 1:6799431/76‑1 (MQ=255) tGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTggg > 2:3506427/1‑76 (MQ=255) ggACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGt < 2:2222005/76‑1 (MQ=255) ggACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGt < 1:328093/76‑1 (MQ=255) ggACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGt < 1:4609847/76‑1 (MQ=255) gACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTa > 1:3270275/1‑76 (MQ=255) aCGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTAt < 1:5816071/76‑1 (MQ=255) ggCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCa < 1:3837103/76‑1 (MQ=255) gCAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCaa < 1:4811491/76‑1 (MQ=255) cAACCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAAc > 1:1701710/1‑76 (MQ=255) aaCCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACa > 2:338390/1‑76 (MQ=255) aaCCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACa > 2:240358/1‑76 (MQ=255) aaCCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACa > 1:4876558/1‑76 (MQ=255) aaCCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACa > 1:4546560/1‑76 (MQ=255) aCCATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACAt < 1:6930593/76‑1 (MQ=255) cATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg > 2:121942/1‑76 (MQ=255) cATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg > 2:3441726/1‑76 (MQ=255) cATCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCg > 1:6253297/1‑76 (MQ=255) aTCAGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGa > 1:81244/1‑76 (MQ=255) aGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGaaaa > 1:1831302/1‑74 (MQ=255) aGATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACt < 1:6471467/76‑1 (MQ=255) gATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTg > 1:6735142/1‑76 (MQ=255) gATGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTg < 2:6363539/76‑1 (MQ=255) aTGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTgg < 2:5904238/76‑1 (MQ=255) aTGGAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTgg < 1:6551353/76‑1 (MQ=255) ggAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa < 2:87861/76‑1 (MQ=255) ggAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa < 2:5090107/76‑1 (MQ=255) ggAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa < 2:5256337/76‑1 (MQ=255) ggAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa < 2:3016694/76‑1 (MQ=255) ggAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa > 1:7064567/1‑76 (MQ=255) ggAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGaa < 1:4215510/76‑1 (MQ=255) gAGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAg < 2:2173775/76‑1 (MQ=255) aGCCGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGa > 2:4960188/1‑76 (MQ=255) ccGGGCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACg > 2:2294452/1‑76 (MQ=255) ggCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGt < 2:1523272/76‑1 (MQ=255) gCATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTa < 2:5326222/76‑1 (MQ=255) cATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTaa < 1:6307781/76‑1 (MQ=255) cATGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTaa < 2:6015692/76‑1 (MQ=255) aTGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAAt > 1:1164019/1‑76 (MQ=255) tGCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAAtt < 1:5363944/76‑1 (MQ=255) gCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtc < 1:4755088/76‑1 (MQ=255) gCCAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtc < 2:1425891/76‑1 (MQ=255) ccAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtct < 2:4047408/76‑1 (MQ=255) cAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtctc < 1:857747/76‑1 (MQ=255) cAGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtctc > 2:4930252/1‑76 (MQ=255) aGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCg < 1:619207/76‑1 (MQ=255) aGAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCg > 1:4377300/1‑76 (MQ=255) gAATCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGc < 1:6912798/76‑1 (MQ=255) aaTCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCt > 2:2567636/1‑76 (MQ=255) aaTCCCTCAACGTATTGTTGAAAGAGATTCGATCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCt > 2:5474528/1‑76 (MQ=255) aTCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTc > 2:2771506/1‑76 (MQ=255) aTCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTc < 2:3645004/76‑1 (MQ=255) aTCCCTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTc < 1:2497872/76‑1 (MQ=255) ccTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCaa > 2:3046020/1‑76 (MQ=255) | GAACGGTCGTACCAAGATGTATAAAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAA > REL606/4164749‑4164895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |