| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,165,628 | T→A | L223Q (CTG→CAG) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,165,628 | 0 | T | A | 99.2% | 1100.9 / ‑1.4 | 261 | L223Q (CTG→CAG) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base A (121/138); total (121/140) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
Only 100 of 265 total aligned reads displayed.
GAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAG > REL606/4165555‑4165702 | gAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGt < 2:721005/76‑1 (MQ=255)gAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGt < 1:4097447/76‑1 (MQ=255) aaCAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGtt < 1:2548946/76‑1 (MQ=255) aCAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTg < 2:4233913/76‑1 (MQ=255) cAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTgg < 1:633875/76‑1 (MQ=255) aGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGa > 2:2687857/1‑76 (MQ=255) aGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGa < 1:1123314/76‑1 (MQ=255) aGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGa > 1:3443901/1‑76 (MQ=255) gTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGaa < 2:565039/76‑1 (MQ=255) tGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGc > 2:2737061/1‑76 (MQ=255) gCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcg > 1:2279742/1‑76 (MQ=255) gCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcg < 2:6444542/76‑1 (MQ=255) cGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcgt < 1:6960191/76‑1 (MQ=255) gTGAAGAGCTGAACGGAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcgtt > 1:3912769/1‑76 (MQ=255) gTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcgtt < 2:638032/76‑1 (MQ=255) tGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcgttc > 1:5750118/1‑76 (MQ=255) gAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcgttcg > 1:475357/1‑76 (MQ=255) gAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcgttcg < 1:3225220/76‑1 (MQ=255) aaGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcgttcgt > 1:4896437/1‑76 (MQ=255) aaGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcgttcgt < 1:283099/76‑1 (MQ=255) aaGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcgttcgt < 2:6843829/76‑1 (MQ=255) agagCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGcgttcgtt < 2:3472958/76‑1 (MQ=255) gagCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCgttcgttc < 1:824389/76‑1 (MQ=255) cTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGt < 2:3150803/76‑1 (MQ=255) cTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGt < 1:2581565/76‑1 (MQ=255) gAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCt > 1:3001693/1‑76 (MQ=255) gAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCt < 1:1817072/76‑1 (MQ=255) aaaCGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCt < 1:3993525/75‑1 (MQ=255) aaCGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTg > 1:5727273/1‑76 (MQ=255) aaCGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTg < 1:968901/76‑1 (MQ=255) aaCGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTg > 1:1172168/1‑76 (MQ=255) cGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGt < 2:1979862/76‑1 (MQ=255) cGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGt < 2:5384302/76‑1 (MQ=255) gAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTa < 1:5135682/76‑1 (MQ=255) gAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTa < 2:2768782/76‑1 (MQ=255) gAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTa < 1:420187/76‑1 (MQ=255) gAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTa < 2:3410884/76‑1 (MQ=255) gAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTa < 2:5728377/76‑1 (MQ=255) gAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTa > 1:1751337/1‑76 (MQ=255) aaaCCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTaa < 2:1175904/76‑1 (MQ=255) aaaCCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTaa < 1:1200318/76‑1 (MQ=255) aaCCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAAc > 1:776343/1‑76 (MQ=255) aCCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACa < 1:6983699/76‑1 (MQ=255) aCCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACa < 2:5456318/76‑1 (MQ=255) aCCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACa < 2:6819944/76‑1 (MQ=255) ccAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACaa < 1:6270879/76‑1 (MQ=255) aaCTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAAc > 2:490031/1‑76 (MQ=255) aaCTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAAc > 2:5241489/1‑76 (MQ=255) cTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCa < 1:4534917/76‑1 (MQ=255) tCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCag < 1:6732662/76‑1 (MQ=255) tCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCag < 2:1518607/76‑1 (MQ=255) tCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCag < 2:1838683/76‑1 (MQ=255) cGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCagag > 2:4207472/1‑76 (MQ=255) cGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCagag > 2:3848293/1‑76 (MQ=255) cGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCagag > 2:6038664/1‑76 (MQ=255) aaaCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTg > 1:894943/1‑76 (MQ=255) aaaCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTg > 2:5043931/1‑76 (MQ=255) aaaCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTg < 1:3795669/76‑1 (MQ=255) aaCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTgg > 2:4346977/1‑76 (MQ=255) aaCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTgg < 2:824102/76‑1 (MQ=255) aaCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTgg < 2:4626199/76‑1 (MQ=255) cAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatg < 1:250118/76‑1 (MQ=255) cAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatg > 1:1623314/1‑76 (MQ=255) cAAGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatg < 1:5425950/76‑1 (MQ=255) aGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatgat < 2:970260/76‑1 (MQ=255) aGCGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatgat < 2:3805753/76‑1 (MQ=255) cGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATcc > 2:5091055/1‑76 (MQ=255) cGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATcc > 1:3839505/1‑76 (MQ=255) cGTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATcc > 1:3739299/1‑76 (MQ=255) gTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCt < 2:165341/76‑1 (MQ=255) gTAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCt > 2:3204734/1‑76 (MQ=255) tAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTg > 2:4525364/1‑76 (MQ=255) tAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTg > 1:6102500/1‑76 (MQ=255) tAAAAAGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTg < 2:176052/76‑1 (MQ=255) aaaaaGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGa < 1:6409069/76‑1 (MQ=255) aaaaaGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGa > 2:1946711/1‑76 (MQ=255) aaaaaGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGa < 1:3318752/76‑1 (MQ=255) aaaaGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGAc > 2:4387667/1‑76 (MQ=255) aaaGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGCGTGGATGATCCTGAcc > 2:2350993/1‑76 (MQ=255) aaaGCTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGAcc < 2:2320861/76‑1 (MQ=255) cTGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTc > 1:6212208/1‑76 (MQ=255) tGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCt < 1:6609909/76‑1 (MQ=255) tGACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCt > 1:172685/1‑76 (MQ=255) gACCAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTg > 2:6895215/1‑76 (MQ=255) cAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCg > 1:1392184/1‑76 (MQ=255) cAAGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCg > 2:1354584/1‑76 (MQ=255) aGCGTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGt < 1:5978427/76‑1 (MQ=255) gTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACt < 2:4822850/76‑1 (MQ=255) gTATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACt < 1:202547/76‑1 (MQ=255) tATCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTg > 1:5119512/1‑76 (MQ=255) aTCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgc > 1:4441841/1‑76 (MQ=255) aTCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgc > 2:885314/1‑76 (MQ=255) tCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgcc > 1:5243160/1‑76 (MQ=255) tCAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgcc > 2:4837593/1‑76 (MQ=255) cAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgccg > 2:1294020/1‑76 (MQ=255) cAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgccg > 2:228008/1‑76 (MQ=255) cAAACAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgccg > 2:315824/1‑76 (MQ=255) aaaCAGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgccgc > 2:3688945/1‑76 (MQ=255) aaCGGTTGGAACCTGTCGTTCACCTTGGTAACAAGCCAGAGTGGATAATCCTGACCGTTCTGCCGGTACTgccgcc < 2:4135986/76‑1 (MQ=255) cTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAg < 1:2160981/76‑1 (MQ=255) | GAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAG > REL606/4165555‑4165702 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |