| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,273,299 | A→T | intergenic (+227/‑115) | nrfG → / → gltP | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,273,299 | 0 | A | T | 99.1% | 923.9 / ‑3.1 | 216 | intergenic (+227/‑115) | nrfG/gltP | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base T (132/82); total (132/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 219 total aligned reads displayed.
CGTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCG > REL606/4273226‑4273371 | cgTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTc < 1:2512535/76‑1 (MQ=255) gTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCa < 1:126548/76‑1 (MQ=255) gTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCa < 2:4020133/76‑1 (MQ=255) ttattaTGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCAt > 1:1398303/1‑76 (MQ=255) ttattaTGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCAt > 2:5832295/1‑76 (MQ=255) ttattaTGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCAt < 2:4774661/76‑1 (MQ=255) ttattaTGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCAt > 2:3291789/1‑76 (MQ=255) tattatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATa > 2:6232108/1‑76 (MQ=255) tattatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATa < 2:5551276/76‑1 (MQ=255) tattatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATa > 2:2264534/1‑76 (MQ=255) attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATaa > 1:5804669/1‑76 (MQ=255) attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATaa < 1:947437/76‑1 (MQ=255) attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATaa < 2:384224/76‑1 (MQ=255) ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 1:3428524/76‑1 (MQ=255) ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 2:4114561/76‑1 (MQ=255) ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg > 2:4199202/1‑76 (MQ=255) ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 2:6269709/76‑1 (MQ=255) tatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGc > 2:2960673/1‑76 (MQ=255) tatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGc > 1:4806033/1‑76 (MQ=255) tatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGc > 1:1235943/1‑76 (MQ=255) tatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGc > 1:2942218/1‑76 (MQ=255) tGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGt > 1:6637552/1‑76 (MQ=255) cATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑a < 1:4818334/76‑1 (MQ=255) cATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑a < 2:6473345/76‑1 (MQ=255) aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑aaaaa > 1:683119/1‑76 (MQ=255) aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑aaaaa > 2:5269477/1‑76 (MQ=255) tGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑aaaaaaa > 1:317981/1‑76 (MQ=255) cAGCGAAAAGCTCTGTTGTTAATGGGTTGTGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATc > 2:4837982/1‑76 (MQ=255) aGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCt > 1:567021/1‑76 (MQ=255) aGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCt > 2:4654573/1‑76 (MQ=255) gCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTa < 2:3066718/76‑1 (MQ=255) gCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTa < 2:6810905/76‑1 (MQ=255) cGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTAc < 1:3674177/76‑1 (MQ=255) cGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTAc < 1:542835/76‑1 (MQ=255) gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACa < 1:234621/76‑1 (MQ=255) aaaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACaa > 2:3687756/1‑76 (MQ=255) aaaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACaa < 1:4844732/76‑1 (MQ=255) aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACaaa < 1:2014363/76‑1 (MQ=255) aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACaaa > 1:608738/1‑76 (MQ=255) aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACaaa > 1:5316038/1‑76 (MQ=255) aaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAAc < 2:1387668/76‑1 (MQ=255) gCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCa < 2:167879/76‑1 (MQ=255) ctctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCaa < 2:5717038/76‑1 (MQ=255) ctctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCaa < 1:2806880/76‑1 (MQ=255) tctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAAc > 1:4489176/1‑76 (MQ=255) ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACg < 1:71146/76‑1 (MQ=255) ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACg > 1:2615468/1‑76 (MQ=255) ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACg < 2:271472/76‑1 (MQ=255) ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACg < 1:1850681/76‑1 (MQ=255) ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACg > 1:5024191/1‑76 (MQ=255) ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACg > 1:4079029/1‑76 (MQ=255) gttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAaca > 1:7009691/1‑76 (MQ=255) gttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAaca < 2:6670785/76‑1 (MQ=255) tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAAcaca > 1:3089192/1‑76 (MQ=255) tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAAcaca > 1:1279439/1‑76 (MQ=255) tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAAcaca > 2:1779379/1‑76 (MQ=255) aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAAATCTACAAACCAACGCAAcaca > 2:3979078/1‑76 (MQ=255) aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACaa > 1:3009704/1‑76 (MQ=255) aaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAAt > 2:4942636/1‑76 (MQ=255) gggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTc < 1:4247403/76‑1 (MQ=255) gggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTc < 1:4152938/76‑1 (MQ=255) gggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACACAACACAATTc < 2:747786/76‑1 (MQ=255) ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCa > 1:3129036/1‑76 (MQ=255) gTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCAt > 2:2309567/1‑76 (MQ=255) gTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCAt < 2:3678013/76‑1 (MQ=255) gTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCAt > 2:2572252/1‑76 (MQ=255) ttGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATg > 2:1574570/1‑76 (MQ=255) ttGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATg > 2:4579022/1‑76 (MQ=255) tGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGc > 2:6334705/1‑76 (MQ=255) tGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGc > 2:1133405/1‑76 (MQ=255) gcgcAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGcc > 2:2776140/1‑76 (MQ=255) gcgcAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGcc > 1:2086292/1‑76 (MQ=255) cgcAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGccc > 2:338968/1‑76 (MQ=255) gcAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATTTACAAACCAACGCAACACAATTCATGCCCt < 1:4408594/76‑1 (MQ=255) gcAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt > 1:4847775/1‑76 (MQ=255) gcAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt < 1:5771878/76‑1 (MQ=255) cAACATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTg > 2:4881817/1‑76 (MQ=255) aaCATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTgg > 2:6065618/1‑76 (MQ=255) aCATACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGc < 1:142587/76‑1 (MQ=255) cATACCGCGCAAATGATACTGATCATAAGCGTT‑AAAACAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa < 1:1655213/76‑1 (MQ=255) aTACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAg > 2:1517326/1‑76 (MQ=255) aTACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAg > 2:6416837/1‑76 (MQ=255) tACCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGt > 1:1012329/1‑76 (MQ=255) aCCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTa > 1:4603306/1‑76 (MQ=255) aCCGCGCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTa > 1:457964/1‑76 (MQ=255) cgcgCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATg < 1:6342751/76‑1 (MQ=255) cgcgCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATg < 2:6941442/76‑1 (MQ=255) cgcgCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATg < 2:6922133/76‑1 (MQ=255) cgcgCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATg < 2:1080419/76‑1 (MQ=255) cgcgCAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATg < 2:1930353/76‑1 (MQ=255) gcgcAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGt < 2:4096388/76‑1 (MQ=255) gcgcAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGt > 2:3531885/1‑76 (MQ=255) gcAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCa > 1:6530615/1‑76 (MQ=255) gcAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCa > 1:3410099/1‑76 (MQ=255) cAAATGATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTAAc < 1:4349844/76‑1 (MQ=255) gATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct > 2:5759184/1‑76 (MQ=255) gATACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct > 1:389175/1‑76 (MQ=255) aTACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 1:3150715/1‑76 (MQ=255) aTACTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 2:5235157/1‑76 (MQ=255) aCTGTTCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcgc > 1:5508141/1‑76 (MQ=255) | CGTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTT‑AAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCG > REL606/4273226‑4273371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |