| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 99.4% | 613.4 / NA | 161 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (55/105); minor base C (1/0); total (56/105) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 190 total aligned reads displayed.
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC > REL606/2193706‑2193850 | ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATccc < 1:589415/76‑1 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATcccc < 2:4655050/76‑1 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATcccc < 2:5440234/76‑1 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAAACAGCCCCCATAA‑CAGCAAAACGCCAATcccc > 1:5039462/1‑76 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTTGCTGGCCGAAATCAACACCCATAACCAGC‑AAACCCCAATcccc > 1:4292376/1‑76 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAATCAGCCCCCATAA‑CAGCCaaaccccaaacccc > 1:5681142/1‑76 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAACCCCCATAA‑CAGCAaaaccccaaacccc > 1:4163792/1‑76 (MQ=255) gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCcca < 1:5211378/76‑1 (MQ=255) gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCcca < 1:893470/76‑1 (MQ=255) gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCCATAA‑CAGCCAAACACCCAACCcca > 1:774980/1‑76 (MQ=255) gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAAACAGACCCCATAA‑AAACAAAACACCAAAccccc > 2:1826117/1‑75 (MQ=255) caTGCATCCGACGTCCAGGCGCGGCCGGTCTGGCTGACCGAAAACAGCCCCAACAA‑CGGCAAAACACCAATCCcca < 2:1744065/75‑1 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGTTTGGCTGCCGGTAACGAACCCCCCTAA‑AAGCAAAACTCCAACcccccc > 1:7006132/1‑74 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCccac < 1:3769113/76‑1 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAATCAGCCCCCATAA‑CAGCAAAACACCAAAcccccc > 1:3159422/1‑74 (MQ=255) tGCACCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCccacc < 2:5190766/76‑1 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAg < 2:5718592/76‑1 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAg < 1:6188379/76‑1 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAg < 2:4949933/76‑1 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGCCTGGCTGTCGGAAAACAGACCCCATAA‑AAGCAAAAAGCCAAAACCCACCAg > 1:6366679/1‑76 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGAt < 1:1274567/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCCACCCCAATAA‑CAGCAAAACGCCAAACCCCACCAGaca > 1:3450761/1‑74 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATa < 2:5330242/76‑1 (MQ=255) cGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAATCAACCCCCATAA‑CAACAAAAAGCCCAACCCCCCCACATaa > 2:823199/1‑76 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGGCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATaaa < 2:6799372/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAAACAGCCCCAAAAA‑CAGCAAAACGCCCATCCCCACCCGATaaa > 2:2256731/1‑76 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAg < 1:1941824/76‑1 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAg < 2:252394/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCCCCAGATAAAGc < 1:6046886/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 2:1329528/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 1:3536107/76‑1 (MQ=255) tCCAGGCGCGGCTGGGCTGGCTGTCGGAAAACAGCCCCCATAA‑AAGCAAAAAGCCAAACCCCCCCCGATAAAACAt > 2:4600218/1‑76 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 2:1641193/76‑1 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 1:1043708/76‑1 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 2:6007211/76‑1 (MQ=255) ggCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg < 2:4238299/76‑1 (MQ=255) cgcgGCTGGGCTGGCTGTCGGAAAACAGCCCCCATAA‑AAGCAAAAAGCCAAACCCCACCAGATAAAAAATGACGcc > 2:1864300/1‑76 (MQ=255) gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 2:5395475/76‑1 (MQ=255) ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt > 2:4805003/1‑76 (MQ=255) ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt > 1:7055561/1‑76 (MQ=255) gCTGGTCGGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa < 2:6489664/76‑1 (MQ=255) cTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCCAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt > 2:6058422/1‑76 (MQ=255) cTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 2:1186252/76‑1 (MQ=255) cTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 2:5403563/76‑1 (MQ=255) tGGTCTGGCTGTCGGGAATCAGCCCCAATAA‑CAGCAAAATGCCAATCCCCACCAGATAAAGCATGATGCCTGTAtt > 2:1124636/1‑76 (MQ=255) tGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:1822730/76‑1 (MQ=255) tGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt > 2:7068185/1‑76 (MQ=255) ggTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 2:6665880/76‑1 (MQ=255) tCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa > 2:560795/1‑76 (MQ=255) tCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 2:366756/76‑1 (MQ=255) tCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 1:106935/76‑1 (MQ=255) tCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 2:1658793/76‑1 (MQ=255) tCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 1:462465/76‑1 (MQ=255) tCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 1:3972917/76‑1 (MQ=255) cTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 2:1041212/1‑76 (MQ=255) cTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 1:655334/1‑76 (MQ=255) cTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 1:291339/1‑76 (MQ=255) tggctggcGGAAAGCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCAACAGATAAAGAATGACGCCTGTATTAAACa > 2:299755/1‑76 (MQ=255) tGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa < 2:6927643/76‑1 (MQ=255) ggCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 2:881930/76‑1 (MQ=255) ggCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 1:6798528/76‑1 (MQ=255) tGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 2:1988587/1‑76 (MQ=255) tGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 1:322111/1‑76 (MQ=255) gTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt > 2:574416/1‑76 (MQ=255) tCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 1:2375279/76‑1 (MQ=255) tCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 1:2046033/76‑1 (MQ=255) cGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGTCtttt < 2:4321306/76‑1 (MQ=255) cGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt < 2:2559367/76‑1 (MQ=255) cGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 1:5540166/1‑76 (MQ=255) ggAAATCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:6222997/76‑1 (MQ=255) aaaTCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 1:4063249/1‑76 (MQ=255) aTCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg > 2:2396389/1‑76 (MQ=255) aTCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg > 1:5303705/1‑76 (MQ=255) tCAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa > 2:4016554/1‑76 (MQ=255) cAGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt > 2:4157365/1‑76 (MQ=255) aGCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg < 1:361778/76‑1 (MQ=255) gCCCCAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg < 1:6955553/76‑1 (MQ=255) ccccAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc < 2:141355/76‑1 (MQ=255) cccAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg < 1:4420344/76‑1 (MQ=255) cccAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg > 1:2411297/1‑76 (MQ=255) ccAATAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc > 2:6375770/1‑76 (MQ=255) aaTAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt < 1:1686103/76‑1 (MQ=255) tAA‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg > 1:5461445/1‑76 (MQ=255) aa‑CAGCAAAAGGCCCACCCCTGCCAGCTAAAGCATGACGCCTGTCTTACACAGGCGTTTTACCGAAGGCGCATcga > 2:4619956/1‑75 (MQ=255) aa‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:213818/1‑76 (MQ=255) aa‑CAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:2629652/1‑76 (MQ=255) cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc < 2:2044695/76‑1 (MQ=255) cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc > 1:3362588/1‑76 (MQ=255) aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa < 2:3981976/76‑1 (MQ=255) aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa < 1:4863986/76‑1 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc > 2:6360141/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:3569642/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 1:1512611/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAGGGCGCATCGCGCACCa < 2:6953225/76‑1 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 1:133637/76‑1 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 2:1064344/76‑1 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg > 2:3717307/1‑76 (MQ=255) aaaCACCAACCCCCACCAGATAAAGCATGACGTCTGTATTAAGCAGCCTGTTTACCGAGGGCGCATCGCGCACCAg < 2:3160486/76‑1 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc > 2:5937057/1‑76 (MQ=255) aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc < 1:6338989/76‑1 (MQ=255) | GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAA‑CAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC > REL606/2193706‑2193850 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |