| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,338,860 | A→C | R230S (AGA→AGC) | yhdG → | tRNA‑dihydrouridine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,338,860 | 0 | A | C | 99.2% | 1093.8 / ‑5.0 | 264 | R230S (AGA→AGC) | yhdG | tRNA‑dihydrouridine synthase B |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (103/159); total (104/160) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 284 total aligned reads displayed.
CCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAG > REL606/3338785‑3338932 | cGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGccc < 1:2169186/76‑1 (MQ=255) gCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGcccc > 1:3566607/1‑76 (MQ=255) cTTAACGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCCCAGCTCAGGGAAGCCCCt < 1:529894/76‑1 (MQ=255) cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCCGGGAAGCCCCt > 1:1360314/1‑76 (MQ=255) cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCt < 1:5772299/76‑1 (MQ=255) cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCt < 1:1261973/76‑1 (MQ=255) cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCt < 2:4714422/76‑1 (MQ=255) ttAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATTATAAGGAACGCCGCTCAGGGAACCCCCtc > 2:5399173/1‑75 (MQ=255) tAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTgg < 2:1137035/76‑1 (MQ=255) tAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTgg < 1:3843776/76‑1 (MQ=255) tAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTgg > 1:4834080/1‑76 (MQ=255) aaaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGa < 1:2016616/76‑1 (MQ=255) aaaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGa > 1:6353222/1‑76 (MQ=255) aaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGAt < 2:1308984/76‑1 (MQ=255) aaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGAt < 2:6851786/76‑1 (MQ=255) aGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCCGGGAAGCCCCTGGATc > 2:5925281/1‑76 (MQ=255) cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCttt > 2:1735779/1‑76 (MQ=255) cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCttt > 2:7025376/1‑76 (MQ=255) agagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTc < 2:6544407/76‑1 (MQ=255) agagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTc < 1:625824/76‑1 (MQ=255) agagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTc < 1:1855243/76‑1 (MQ=255) gCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCggg > 1:5565238/1‑76 (MQ=255) gCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCggg < 1:1596930/76‑1 (MQ=255) cTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGa > 1:3584750/1‑76 (MQ=255) cTGTGCTCGACTATACAGGGGCGGAAGCCCCGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCCTTCgggg > 1:4598942/1‑75 (MQ=255) gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGGCGCGCCACTCCGGGAAGGCCCTGGATCTTTCCGGgaa > 2:6496714/1‑76 (MQ=255) gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGaaa > 1:2330875/1‑76 (MQ=255) gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGaaa > 1:6423064/1‑76 (MQ=255) tgCTCGACTATACAGGTGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAAt > 1:4040421/1‑76 (MQ=255) tgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGGAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAAt > 1:6089069/1‑76 (MQ=255) tgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAAt < 1:4002934/76‑1 (MQ=255) tCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCa < 1:5917904/76‑1 (MQ=255) tCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGCGAAATCCa < 1:4173624/76‑1 (MQ=255) cGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAg < 2:2133597/76‑1 (MQ=255) cGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAg < 1:4330743/76‑1 (MQ=255) gACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATACAGc > 1:5341992/1‑76 (MQ=255) aCTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCa < 2:419459/76‑1 (MQ=255) aCTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCa < 2:6631229/76‑1 (MQ=255) cTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCAt < 2:817761/76‑1 (MQ=255) tataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCAtt < 1:2265296/76‑1 (MQ=255) ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTa < 1:56126/76‑1 (MQ=255) ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTa > 2:6649378/1‑76 (MQ=255) ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTa < 2:5860743/76‑1 (MQ=255) taCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTAt < 1:2945532/76‑1 (MQ=255) cAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCt > 1:5429651/1‑76 (MQ=255) cAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCt < 1:6565010/76‑1 (MQ=255) cAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCt < 2:4064059/76‑1 (MQ=255) aGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTg < 1:6127212/76‑1 (MQ=255) aGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTg < 2:1497629/76‑1 (MQ=255) gggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGa < 2:4868479/76‑1 (MQ=255) gggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGa < 1:728076/76‑1 (MQ=255) gggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGa < 2:2366220/76‑1 (MQ=255) ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac > 1:824731/1‑76 (MQ=255) ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac > 1:5422678/1‑76 (MQ=255) ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac < 1:2291176/76‑1 (MQ=255) ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac < 1:3126858/76‑1 (MQ=255) cGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGacac > 1:1799086/1‑76 (MQ=255) ggATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACt < 2:3747037/76‑1 (MQ=255) aTGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgg > 2:5574817/1‑76 (MQ=255) tGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTggg > 1:750291/1‑76 (MQ=255) gCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg > 1:1515691/1‑76 (MQ=255) gCCCTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg > 2:4890854/1‑76 (MQ=255) cccTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGa < 2:5791506/76‑1 (MQ=255) cccTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGa < 2:2533691/76‑1 (MQ=255) ccTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg > 2:4512570/1‑76 (MQ=255) ccTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg < 1:4521126/76‑1 (MQ=255) cTGATGATAGGCCGCGCAGCTCAGGGAAGCGCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGt > 2:3888146/1‑76 (MQ=255) cTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGt > 1:5218197/1‑76 (MQ=255) cTGATGATAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGt > 2:4565205/1‑76 (MQ=255) tgatgaTAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt > 2:2592694/1‑76 (MQ=255) atgatAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGATACTGGGGAGTtgc > 1:3398441/1‑76 (MQ=255) atgatAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgc < 2:4958761/76‑1 (MQ=255) tgatAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgct > 1:3701913/1‑76 (MQ=255) atAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgctgc > 1:4089139/1‑76 (MQ=255) atAGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgctgc > 2:4010811/1‑76 (MQ=255) aGGCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccc < 2:4296060/76‑1 (MQ=255) gCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccccc < 2:5733752/76‑1 (MQ=255) gCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccccc < 1:1892477/76‑1 (MQ=255) gCCGCGCAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccccc < 1:268975/76‑1 (MQ=255) gcgcAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCt < 2:2697198/76‑1 (MQ=255) cgcAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTg < 1:6797181/76‑1 (MQ=255) cgcAGCCCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTg < 1:3344179/76‑1 (MQ=255) cAGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGcc > 1:885684/1‑76 (MQ=255) aGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCt > 2:3940958/1‑76 (MQ=255) aGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCt > 2:337198/1‑76 (MQ=255) aGCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCt > 2:5986893/1‑76 (MQ=255) gCTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCtt < 2:3551048/76‑1 (MQ=255) cTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt < 2:5247036/76‑1 (MQ=255) cTCAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt < 1:1011216/76‑1 (MQ=255) cAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 2:4822275/1‑76 (MQ=255) cAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 1:2535818/1‑76 (MQ=255) cAGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 2:6822776/1‑76 (MQ=255) aGGGAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGc > 2:3079842/1‑76 (MQ=255) gggAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCa < 1:2820597/76‑1 (MQ=255) ggAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCag < 1:4656222/76‑1 (MQ=255) ggAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCag < 2:1997552/76‑1 (MQ=255) ggAAGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTNGGCag < 2:2253035/76‑1 (MQ=255) aaGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCagag < 1:1043286/76‑1 (MQ=255) aaGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCagag < 2:6321934/76‑1 (MQ=255) aaGCCCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCagag < 2:4622584/76‑1 (MQ=255) | CCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAG > REL606/3338785‑3338932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |