| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,759,444 | 0 | A | C | 38.0% | 298.7 / 26.0 | 142 | H261P (CAC→CCC) | ydiS | predicted oxidoreductase with FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base A (75/13); new base C (0/54); total (75/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.03e-27 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 199 total aligned reads displayed.
GGCTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCGGTC > REL606/1759369‑1759518 | ggCTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAacaaca < 1:6324371/76‑1 (MQ=255) cTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACAcc > 2:6785192/1‑76 (MQ=255) cTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACAcc < 2:4848252/76‑1 (MQ=255) ggTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACAcccc < 2:1478101/76‑1 (MQ=255) gTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCg > 1:6929985/1‑76 (MQ=255) ttGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGcc > 1:1053999/1‑76 (MQ=255) ttGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGcc > 1:2182532/1‑76 (MQ=255) ttGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGcc < 2:1259617/76‑1 (MQ=255) ttGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGcc > 1:1701823/1‑76 (MQ=255) ttGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGcc < 2:32446/76‑1 (MQ=255) tgtgGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCa < 1:4858321/76‑1 (MQ=255) gtgGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCAt < 1:2964379/76‑1 (MQ=255) tgGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACCACACACCGCCAtt > 1:2806435/1‑76 (MQ=255) ggATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTc < 1:4681278/76‑1 (MQ=255) gATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCg < 1:6714343/76‑1 (MQ=255) gATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCg > 1:6035818/1‑76 (MQ=255) gATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCGGGAAGATTTTAAACAACCCCCCGCCATTCg < 2:1412473/76‑1 (MQ=255) ttGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGcc < 2:3518975/76‑1 (MQ=255) gggTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCg > 1:905390/1‑76 (MQ=255) gTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCt < 1:2894444/76‑1 (MQ=255) tGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTg > 2:5215884/1‑76 (MQ=255) tGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTg > 2:5082631/1‑76 (MQ=255) tGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAAGGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTg < 2:2536624/76‑1 (MQ=255) gATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGa > 1:2583926/1‑76 (MQ=255) gATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGa > 1:5375591/1‑76 (MQ=255) tatCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGAtt < 2:1658216/76‑1 (MQ=255) atCGCCCATGCGCAAAAAAGC‑GTCCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTa < 2:2741928/76‑1 (MQ=39) atCCCCCATGCGAAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTa < 1:4105568/76‑1 (MQ=37) tCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAg > 1:351513/1‑76 (MQ=255) cccccATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAgc < 2:4498133/74‑1 (MQ=255) cccccATGCGAAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAgc < 2:3678268/74‑1 (MQ=37) cGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAgc > 1:1045480/1‑76 (MQ=255) cGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAgc > 1:5461736/1‑76 (MQ=255) cGCCCAGGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAgc < 1:2011823/76‑1 (MQ=255) gCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAgcg > 2:174533/1‑76 (MQ=255) cccATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAgcgg > 2:471487/1‑76 (MQ=255) cccATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAgcgg > 1:716953/1‑76 (MQ=255) cccATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAgcgg > 2:4462567/1‑76 (MQ=255) ccATGCGCAAAAAAGG‑GTACCGCAAATGCTGAAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAgcggc < 1:5559345/76‑1 (MQ=38) ccATGCGAAAAAAAGC‑GGGCCGCAAATGCTGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAgcggc < 2:4262720/76‑1 (MQ=37) caggcgcAAAAAAGC‑GTGCCGAAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAgcggcg < 2:5205628/73‑1 (MQ=255) caagcgcAAAAAAGC‑GTGCGGAAAATGCGGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAgcggcg < 1:1335947/73‑1 (MQ=255) aTGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGTTTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGcggcgg < 2:5001613/76‑1 (MQ=255) aTGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGcggcgg < 2:6473056/76‑1 (MQ=255) aTGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGcggcgg > 2:396941/1‑76 (MQ=255) aTGCGAAAAAAAGC‑TTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGcggcgg < 1:4988908/76‑1 (MQ=38) tGCGAAAAAAGGC‑GGGCCGAAAATGCGGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAGCggcggc < 2:41506/76‑1 (MQ=255) gcgcAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCa < 2:6811273/76‑1 (MQ=255) cgaaaaaaaGC‑GTGCCGAAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCaa < 2:1123546/73‑1 (MQ=38) ccAAAAAAGG‑GGGCCGCAAATGCGGAAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCaaa < 2:6056376/75‑1 (MQ=255) gcaaaaaGCGGTGCCGCAAATGGTGAAAAATTTTAACCAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCaaa < 2:4546412/74‑1 (MQ=255) aaaaaaaGC‑GGGCCGCAAATGCTGAAAAATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAAc < 2:3862953/75‑1 (MQ=255) aaaaaaGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAAca < 2:2173892/76‑2 (MQ=255) taaaGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGc > 2:3784271/2‑76 (MQ=255) aatgC‑GTGCCGCAAATGCTGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCt < 1:455189/73‑1 (MQ=38) aaGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCtt > 1:3885915/1‑76 (MQ=255) cgC‑GTACAGCAAATGCTGGAAGATTTAAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTg < 2:3309320/75‑1 (MQ=38) gC‑GTGCCGCAAATGCTGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGa < 1:3811863/76‑1 (MQ=255) gC‑GTCCCGCAAATGCTGAAAGTTTTAAACCACCCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGa < 1:3657375/76‑1 (MQ=255) gC‑GGGCCGCAAAGCCTGAAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGa < 2:1859595/76‑1 (MQ=255) gTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGGAAACTGCTTGAat > 2:1325318/1‑76 (MQ=255) gTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAat > 1:2879545/1‑76 (MQ=255) gTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAAATGCTTGAat > 2:2020794/1‑76 (MQ=255) gTGCCGCAAATGCTGAAAGTTTTAAACCACCCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAat < 2:5589506/76‑1 (MQ=255) gTGCCGAAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAat < 2:4963616/76‑1 (MQ=255) gaaaaTGATGGAATGTTTTAACGCCCCCCCCGCCTTTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTcc < 2:4940129/74‑1 (MQ=255) cAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGGAAAATGCTTGAATATTTcg > 2:459360/1‑76 (MQ=39) aaaTGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTTCATATTCcgc > 1:4150746/1‑76 (MQ=255) aaaTGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCcgc > 1:5679077/1‑76 (MQ=255) aaaTGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAAATGCTTGAATATTTcgc > 2:6598693/1‑76 (MQ=255) aggCTGGAAAATTTTAACCACCCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCgcgc < 1:5857647/74‑1 (MQ=255) cTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTTAATATTCCGCGCata > 1:637474/1‑76 (MQ=255) tGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCatat > 1:4383592/1‑76 (MQ=255) tGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCatat > 2:6981676/1‑76 (MQ=255) ggAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATg < 1:528022/76‑1 (MQ=255) ttttAACCACCCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCg < 1:5227312/76‑1 (MQ=255) ttttAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCg < 2:1890982/76‑1 (MQ=255) tttAAACACCCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCgg < 2:5845855/76‑1 (MQ=255) tttAAACACCCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCgg < 1:3147096/76‑1 (MQ=255) ttAACCACCACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGa < 1:2873244/76‑1 (MQ=255) ttAAACAACCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGa < 2:3563306/76‑1 (MQ=255) ttAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGa > 1:1541261/1‑76 (MQ=255) ttAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAAATGCTTGAATATTCCGCGCATATGGTGGCggg > 2:46385/1‑75 (MQ=255) aaaCAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCCCGCCTATGGTGCCGGAAg > 1:5263791/1‑76 (MQ=255) aacaccCCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAgg < 1:1173986/76‑1 (MQ=255) acaacaCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTTCGCGCATATTGTGCCGGAAggg > 2:5532181/1‑75 (MQ=255) acaacaCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAAATGCTTGAATATTTCGCGCCTATGGTGGCGGAAggg > 2:5690589/1‑75 (MQ=255) caacaCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCg > 2:2898658/1‑76 (MQ=255) caacaCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACAGCTTGAATATTCCGCGCATATAGGGCCGGAAgggg > 2:2499504/1‑74 (MQ=255) caacaCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAAATGCTTGAATATTTCGCGCATATGGGGCCCGAAgggg > 2:165384/1‑74 (MQ=255) aaaccCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCg < 2:842480/75‑1 (MQ=255) aacaCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATTGTGCCGGAAGGCgg > 2:1383319/1‑76 (MQ=255) aacaCCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCCCGCATATGGTGCCGGCAGGCgg > 1:6596617/1‑76 (MQ=255) acacCCCGCCATTCGCCCGCTGATTAGCGGCGGCCAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCggg > 1:3307716/1‑75 (MQ=255) cacCCCGCCATTCGCCCGCTGATTAGCGGCGGTAAACTGCTTTAATATTCCCCGCGTATGGTGGCGGGcggcgggc > 2:6294483/1‑74 (MQ=255) cacCCCGCCATTCGCCCGCTGATTAGCGGCGGGAAACTGCTTTAATAATCCGCGCATATGGTGCCGGAAGgggggc > 2:230616/1‑74 (MQ=255) cacCCCGCCATTCGCCCGCTGATTAGCGGCGGGAAACTGCTTGAATATTCCGCGCATATGGTGCCGGGAGGCGGTc > 1:1984661/1‑76 (MQ=255) cacCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTTCTTGAATTTTCTCCGCCTATTGTGACGGAaggagggc > 2:6165703/1‑74 (MQ=255) cacCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGGGGTc > 1:3722633/1‑76 (MQ=255) cacCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCgggc > 1:3415783/1‑74 (MQ=255) | GGCTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGC‑GTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCGGTC > REL606/1759369‑1759518 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |