| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,144,295 | 0 | A | C | 37.7% | 361.0 / 78.8 | 183 | G357G (GGT→GGG) | mrp | antiporter inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (108/6); new base C (0/69); total (108/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.29e-44 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 214 total aligned reads displayed.
CGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGACG > REL606/2144220‑2144369 | gATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCAc > 1:1242186/1‑76 (MQ=255) aTGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCAcc < 2:5527379/76‑1 (MQ=255) gTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCt > 1:4114727/1‑76 (MQ=255) gTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCt > 2:4164031/1‑76 (MQ=255) gTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCt > 1:2706121/1‑76 (MQ=255) gTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCt > 2:6017075/1‑76 (MQ=255) tATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTGACCCTg > 1:3598090/1‑76 (MQ=255) tATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTg > 1:3903379/1‑76 (MQ=255) tGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGcc > 1:4864160/1‑76 (MQ=255) tGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGcc > 1:2477018/1‑76 (MQ=255) aagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAg > 2:1927700/1‑76 (MQ=255) aagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAg < 2:2239617/76‑1 (MQ=255) aaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTag > 2:1218221/1‑76 (MQ=255) aaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTag > 1:3892419/1‑76 (MQ=255) aaCCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAgagc > 2:1526010/1‑76 (MQ=255) aCCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAgagct < 1:5056025/76‑1 (MQ=255) cAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAgagctca > 2:3801887/1‑74 (MQ=255) aaTGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAgagctgagct < 2:5920148/76‑1 (MQ=255) aTGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGagctgagctg < 1:2830475/76‑1 (MQ=255) tGGTTAATGGGCGTAAGCCCGGGCGGAACAAACTCTCCCCTAGAGTGACTCCACCCTGCCAGTAGAGCTGAGCTGc < 1:977560/76‑1 (MQ=255) gTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCaa < 1:6423681/76‑1 (MQ=255) ttAATGGGCGTTAGCCCGCGCGGAAGGAAATCTCGCCGGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAAc < 2:3992255/76‑1 (MQ=255) ttAATAGGCGTTAGCCCGCGCGGAAGGAAATCTCGCCTGGAATGCCTTCCCCCTGCCAGTAGAGCTGAGCTGCAAc < 2:3261482/76‑1 (MQ=255) ttAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAAc < 2:3462080/76‑1 (MQ=255) ttAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGAAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAAc < 2:6438771/76‑1 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgc > 2:4801937/1‑76 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgc > 2:3153558/1‑76 (MQ=255) aTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCATTAGAGCTGAGCTGCAAcgcg > 1:5375249/1‑76 (MQ=255) aTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgcg > 1:3686566/1‑76 (MQ=255) tAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCgg > 1:6563935/1‑76 (MQ=255) aGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 2:6520159/1‑76 (MQ=255) aGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 2:4110307/1‑76 (MQ=255) aGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:638992/1‑76 (MQ=255) ggCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 1:2018876/76‑1 (MQ=255) ggCGTTAGACCGCGCGGAAGGAAATCTCCCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 1:2915142/76‑1 (MQ=255) ggCGTTAGACCGCGCGGAAGAAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 2:6896118/76‑1 (MQ=255) ggCGTTAGACCGCGCGGAAGAAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 1:186741/76‑1 (MQ=255) ggCGTGAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 2:5214411/76‑1 (MQ=255) ggCGTAAGACCGCGCGGAAGGAAATCTCGCCTGAAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 2:3869500/76‑1 (MQ=255) gCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTca > 1:5650928/1‑76 (MQ=255) gCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTca > 1:7083677/1‑76 (MQ=255) cGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcag > 1:4079305/1‑76 (MQ=255) cGTGAGACCGCGCGGAAGGAAATCTCGCCGGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcag < 2:1731186/76‑1 (MQ=255) ttAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcc < 1:4137359/76‑1 (MQ=255) tAGACGGCGGGGAAGGAAACCTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcca < 2:4272703/76‑1 (MQ=255) tAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcca > 1:5216076/1‑76 (MQ=255) tAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcca > 1:2068271/1‑76 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagccag > 2:4889936/1‑76 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagccag > 1:1641764/1‑76 (MQ=255) gcccGCGCGAAAGAAATTCTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagccagc < 1:2522084/74‑1 (MQ=255) ccggcgcgGAAGGAATCCTCGCCGGGAATCCCTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCt < 2:2291899/73‑1 (MQ=255) cccGCGGGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCt < 2:1574589/75‑1 (MQ=255) cccGCGGGGAAGAAAACCTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCt < 2:5892773/75‑1 (MQ=255) ccGCGCGGAAGAAAATCTCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTg < 2:6261534/76‑1 (MQ=255) gccgcgGAAGAAAACCTCGCCGTGAGTCCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTgg < 2:4694880/74‑1 (MQ=255) cgcgcgGAAGGAAATCTCGCCTGGAAGGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTgg < 1:3510030/76‑1 (MQ=255) cgcgcgGAAGAAAATCCCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTgg < 2:202290/76‑1 (MQ=255) gcgcgAAAGAAAACCTCGCCGGAAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGc < 1:649216/76‑1 (MQ=255) gcgGAAGGAAATCTCGCCTGGAATGCCTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCgg < 2:3301896/76‑1 (MQ=255) gcgGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCgg > 2:4555098/1‑76 (MQ=255) gtaaagaaaGTCCCGCCTGGATTGTCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGt < 1:3061187/73‑1 (MQ=255) gggaaggaaATCCCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGt < 1:1454377/75‑1 (MQ=255) gcaaaggaaTTGTCCCCTGGAATAACTTCCCCCTGCCAGTAGACCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGt < 2:5724559/73‑1 (MQ=255) cgGAAGGAATTCTCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGt < 2:1465652/76‑1 (MQ=255) cgGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGt > 2:2608713/1‑76 (MQ=255) cgGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGt > 1:332111/1‑76 (MQ=255) cgGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGt > 2:3415852/1‑76 (MQ=255) cgGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCCGGCGGt > 1:5962355/1‑76 (MQ=255) ggaaggaaATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTa > 2:1083725/1‑76 (MQ=255) ggaaggaaATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTa > 2:3709606/1‑76 (MQ=255) gaaagaaaATCTCGCCTGAAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTa < 2:2815454/74‑1 (MQ=255) gaaggaaCTCTCGCCTGAAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAg < 1:4193238/76‑1 (MQ=255) gaaggaaATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTac > 1:2743235/1‑75 (MQ=255) gaaggaaATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAg > 2:4400714/1‑76 (MQ=255) gaagaaaCTCTCGCCGGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAg < 2:2268631/76‑1 (MQ=255) gaagaaaATCTCGCCTGGAATGCCTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAg < 2:2863568/76‑1 (MQ=255) aaggaaATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGa > 2:816898/1‑76 (MQ=255) aagaaaATCTCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGa < 2:5618964/76‑1 (MQ=255) aggaaATCCGGCCTGGAGTGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGAt < 2:2153224/76‑1 (MQ=255) gaaaaTCCCGCCGGAAATCCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATa < 2:6673514/74‑1 (MQ=255) gaaATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg > 1:1354900/1‑76 (MQ=255) gaaATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg > 2:6584068/1‑76 (MQ=255) aaaCCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGc < 2:5376784/76‑1 (MQ=255) aacctcGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCg < 2:1333862/73‑1 (MQ=255) acctcGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCgg < 1:4483994/74‑1 (MQ=255) aTCTCGCCGGGATTTCCTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCgg < 1:6909666/76‑1 (MQ=255) ctcGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTa > 1:4743728/1‑76 (MQ=255) ctcGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTa > 2:3031594/1‑76 (MQ=255) ctcGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTa > 1:1078648/1‑76 (MQ=255) tcGCCGGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTaa < 2:5513675/76‑1 (MQ=255) cGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTaaa > 1:3819176/1‑76 (MQ=255) cGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTaaa > 2:1963924/1‑76 (MQ=255) gCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTTAAt > 2:2705428/1‑76 (MQ=255) gggAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCg < 2:5900977/75‑1 (MQ=255) ggAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGc > 2:280543/1‑76 (MQ=255) aaTGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGctc < 1:4639802/76‑1 (MQ=255) gACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTcc > 2:5820693/1‑76 (MQ=255) cTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCgg > 1:5560839/1‑76 (MQ=255) cACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGACg > 2:4458406/1‑76 (MQ=255) cACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGACg > 1:4943557/1‑76 (MQ=255) | CGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGACG > REL606/2144220‑2144369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |