| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,096,354 | 0 | A | C | 38.0% | 207.9 / 72.4 | 137 | V163G (GTG→GGG) | sufI | repressor protein for FtsI |
| Reads supporting (aligned to +/- strand): ref base A (79/6); new base C (2/50); total (81/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-28 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 173 total aligned reads displayed.
CCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAG > REL606/3096280‑3096429 | ccTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTccac > 1:6241274/1‑76 (MQ=255)ccTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTccac > 1:3517983/1‑76 (MQ=255) cTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTccacc > 1:4014358/1‑76 (MQ=255) tGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTccacca < 1:4594369/76‑1 (MQ=255) tGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTccacca < 1:4987194/76‑1 (MQ=255) tGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTccacca < 1:657257/76‑1 (MQ=255) ggATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAg > 1:1099628/1‑76 (MQ=255) ggATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAg > 1:3398669/1‑76 (MQ=255) ggATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAg > 1:2726766/1‑76 (MQ=255) ggATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAAGCAGCGAATTT‑CTGGCTTCATCTTccaccaa > 1:318004/1‑75 (MQ=255) aTAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGcc < 2:1556133/76‑1 (MQ=255) aTAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGcc > 1:312441/1‑76 (MQ=255) aaTGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCcaccac > 1:749989/1‑76 (MQ=255) aTGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCCCCAGcccaa > 2:5354771/1‑73 (MQ=255) tGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACAt > 1:6318109/1‑76 (MQ=255) ccGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTcc > 1:862182/1‑76 (MQ=255) cGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAACCACCTTCCg > 1:1449431/1‑76 (MQ=255) aaaaaTCACCCCCCCCATAATGGTGGGGGATGGCCAGCGCCTTG‑CTGACTCCATCTCCCCCCAGCCACATTCCGGc < 2:2116771/75‑1 (MQ=255) aaaTCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCaa > 1:4908172/1‑76 (MQ=255) aaTCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACCTTCCGGCAAg > 1:3153442/1‑76 (MQ=255) aaTCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCCAg > 2:2885752/1‑76 (MQ=255) atcatcCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAgg > 2:6855429/1‑76 (MQ=255) cattCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGcc > 1:683436/1‑76 (MQ=255) catcCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCCCCAGCCACATTCCGGCAAGGcc < 2:1510276/76‑1 (MQ=255) catcCACACCATAAGGGTGGGGGATAGCCAGCGACTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGcc < 1:3769371/76‑1 (MQ=255) catcCACACCAAAATGGTGGGGGATAGGCAGCGACTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGcc < 1:3452775/76‑1 (MQ=255) atcCACCCCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCCCCAGCCACATTCCGGCAAGGCCg < 1:2033241/76‑1 (MQ=255) ccACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGtt > 2:707385/1‑76 (MQ=255) ccACACCATAAGGGGTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGtt < 1:6485312/76‑1 (MQ=255) cacaCCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGttt > 2:2781211/1‑75 (MQ=255) accccATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTtat < 1:6962060/73‑1 (MQ=255) accccATAAGGGTGGGGAATGGGCAGCGACTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTtat < 1:6006227/73‑1 (MQ=255) acacCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGGCTTCATCTTCCACCAGCCACATTCCGGCAAGGGCGTtat > 2:4698444/1‑76 (MQ=255) acacCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCCGCCACATTTCGGCCAGGCCGttct > 1:121068/1‑74 (MQ=255) acacCATAAGGGTGGGGGATAGGCAGCGACTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTtat < 1:6727698/76‑1 (MQ=255) acacCAAAATGGTTGGGGAGAGGCAGCGACTTT‑CTGCCTTCATCTCCCACCAGCCACATTCCGGCAAGGCCGTtat < 1:1808563/76‑1 (MQ=255) cacCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTtata > 1:6285241/1‑76 (MQ=255) cccATAAGGGTGGGGAATAGGCAGCGACTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAg < 1:2858735/75‑1 (MQ=255) acCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAg > 1:2931169/1‑76 (MQ=255) ccATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCCTCTCCCACCAGCCACATTCCGGCCAGGCCGTTATAGa > 1:4764815/1‑76 (MQ=255) ccATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGa > 1:4622507/1‑76 (MQ=255) ccATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGa > 2:2386955/1‑76 (MQ=255) ccAAAAGGGTGGGGGATAGGCAGCGACTTG‑CTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGa < 1:2351985/76‑1 (MQ=255) cATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGAc > 2:2951845/1‑76 (MQ=255) aTAGTGGTGGGGGATAGGCAGCGACTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGAcc < 2:5257921/76‑1 (MQ=255) aTAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGaac > 1:2412997/1‑74 (MQ=255) tAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACct > 2:3527436/1‑76 (MQ=255) aTGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACctgc > 2:1638904/1‑76 (MQ=255) aTGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACctgc > 2:1757753/1‑76 (MQ=255) aTGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACctgc > 1:6676315/1‑76 (MQ=255) ggTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCCAAGCCGTTATAGACctgctg > 2:5809734/1‑76 (MQ=255) ggTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACctgctg > 1:5844998/1‑76 (MQ=255) ggTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGAActgctg > 2:4401158/1‑76 (MQ=255) ggTTGGGGAGGGGCAGCGCCTTG‑CTTATTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACctgctg < 1:2045671/76‑1 (MQ=255) ggTGGGGGAAAGCCAGCGACTTC‑CTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACctgctg < 1:4271446/76‑1 (MQ=255) ggTGGGGAATGGGCAGCGACTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACctgctg < 1:4840848/76‑1 (MQ=255) ggTGGGGAATAGGCAGCTACTTG‑CTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACctgctg < 1:1056316/76‑1 (MQ=255) gTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGa > 1:1800794/1‑76 (MQ=255) tgggggATAGGCGGCG‑CTTGTCTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAg < 1:2412346/74‑1 (MQ=255) tGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGc > 1:1715861/1‑76 (MQ=255) tGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGc > 1:2464693/1‑76 (MQ=255) ggggATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGcc > 2:4656682/1‑76 (MQ=255) gATGGGCGGCGACTTG‑CTGCTTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTg < 2:3361484/76‑1 (MQ=255) gATAGCCGGCGCCTTG‑CTGACTTCTTCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTg < 1:5214143/76‑1 (MQ=255) agaGGCAGCTATTTG‑CTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGc < 2:1645031/74‑1 (MQ=255) aTAGGCGGCGTCTTG‑CTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGc < 1:2232549/76‑1 (MQ=255) aTAGGCAGCTCCTTG‑CGGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGc < 1:3348901/76‑1 (MQ=255) tAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCg > 1:5417692/1‑76 (MQ=255) tAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCg > 2:1282420/1‑76 (MQ=255) aGGCAGCGACTTG‑CTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCgg < 1:5286607/76‑1 (MQ=255) ggCAGCGATTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGt < 2:965503/76‑1 (MQ=255) gCAGCGACTGC‑GTGCTTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGtt < 2:1415758/76‑1 (MQ=255) ccggCGACTTG‑CTGCTTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGtt < 2:5172582/73‑1 (MQ=255) cggCGTCTTG‑CTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTg < 2:2152613/74‑1 (MQ=255) caccGACTTG‑CTGACTTCCTCTTCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTg < 2:7182820/73‑1 (MQ=255) cAGCGACTTG‑CCCCCTCCATCTCCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTg < 2:6022681/76‑1 (MQ=255) aGCGCCTTC‑CGGCCTCCACCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTgg < 1:1317846/76‑1 (MQ=255) gCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTggg > 2:4010981/1‑76 (MQ=255) gCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTggg > 2:6282028/1‑76 (MQ=255) cGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGa > 2:2549864/1‑76 (MQ=255) ccTTG‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGt < 2:4356797/75‑1 (MQ=255) g‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTg > 1:4006720/1‑76 (MQ=255) g‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTg > 2:6318730/1‑76 (MQ=255) g‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGGATTg > 1:4332327/1‑76 (MQ=255) g‑CTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTg < 2:4261971/76‑1 (MQ=255) gACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCg > 2:2874367/1‑76 (MQ=255) gACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCg > 2:644959/1‑76 (MQ=255) gACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCg < 2:7138359/76‑1 (MQ=255) gACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCg > 2:5655502/1‑76 (MQ=255) gACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCg > 1:3287671/1‑76 (MQ=255) gACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCg > 2:3536117/1‑76 (MQ=255) cTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTg > 1:828037/1‑76 (MQ=255) tCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGAt > 2:1894734/1‑76 (MQ=255) tCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGAt > 1:1262913/1‑76 (MQ=255) tCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGAt > 2:3129492/1‑76 (MQ=255) tCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGAt < 1:7098732/76‑1 (MQ=255) aTCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATAc > 2:2647437/1‑76 (MQ=255) cTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACca > 2:1348807/1‑76 (MQ=255) ttCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACcac > 1:2137773/1‑76 (MQ=255) ccaccaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACag > 2:4765369/1‑76 (MQ=255) | CCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTG‑CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAG > REL606/3096280‑3096429 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |