| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,180,249 | 0 | T | G | 46.2% | 110.6 / 31.7 | 80 | G24G (GGT→GGG) | yjaH | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (18/25); new base G (37/0); total (55/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 160 total aligned reads displayed.
GGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAG > REL606/4180174‑4180324 | cAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTgc < 2:4719037/76‑1 (MQ=255) tGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCt < 1:4003393/76‑1 (MQ=255) gAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTg < 2:5537479/76‑1 (MQ=255) gAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGgcgccg > 2:1600762/1‑74 (MQ=255) gAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGgcgccg > 2:3954628/1‑74 (MQ=255) gAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTg > 1:4693694/1‑76 (MQ=255) gAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTg > 1:3542295/1‑76 (MQ=255) aCAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGtt < 1:1407977/76‑1 (MQ=255) cAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGttt < 2:1012498/76‑1 (MQ=255) aGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTtc < 1:3741771/76‑1 (MQ=255) aGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGtttt > 2:668765/1‑75 (MQ=255) ttttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTtctc < 1:1767427/76‑1 (MQ=255) tttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCa > 1:3335204/1‑76 (MQ=255) ttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCAc < 2:4259557/76‑1 (MQ=255) tAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACt > 2:2723381/1‑76 (MQ=255) tAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACt > 2:3415546/1‑76 (MQ=255) aaCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACtc > 2:1177897/1‑75 (MQ=255) aaCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACtt > 1:3180131/1‑75 (MQ=255) cGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGcc > 1:3149039/1‑76 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCt < 2:1344582/76‑1 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGGTGTTTCTTACTGCCt > 2:6133671/1‑76 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCt > 2:1572755/1‑76 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGCTTCTCGCTGCCt > 2:2912523/1‑76 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGGCGTTTCTTACTGCCTg > 1:925232/1‑76 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCcctgcctg > 2:6884058/1‑76 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGGTTCTCACTGCCtt > 1:801622/1‑75 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACTGCCtct > 2:4188408/1‑74 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCTCTGTTTCTCACTGCCTGTa < 2:564140/76‑1 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTa < 1:6288486/76‑1 (MQ=255) ggtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCGGTTTCTCAATTCCTCTAGc > 2:5141561/1‑76 (MQ=255) gtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGcc < 2:2351596/76‑1 (MQ=255) gtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGcc < 2:3399700/76‑1 (MQ=255) gtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGcc < 2:1477532/76‑1 (MQ=255) gtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGcc < 1:1761439/76‑1 (MQ=255) tggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCca < 2:1808036/76‑1 (MQ=255) tggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCCCACTGCCTGTAGCca < 2:6964875/76‑1 (MQ=255) tggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACTGCCTGTAGCca > 1:6730761/1‑76 (MQ=255) tggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGGTTCTCCCTGCCTGTAGccc > 2:796634/1‑75 (MQ=255) ggtTTCCCCCTTTTTGTCTTTGTGGTGTCGTTCATTGATGCTGGAAGGTGCGTTGTTTCTCCCTGCCTTTAGCcac < 2:6517740/76‑1 (MQ=255) ggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCcac < 1:4303627/76‑1 (MQ=255) tttCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGGTGTTTCTCACTGCCTGTAGCCACaa > 1:2579671/1‑76 (MQ=255) ttCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAAc > 1:5261977/1‑76 (MQ=255) tCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCCGCTGCcaccacc > 2:4502216/1‑75 (MQ=255) tCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTCCCTCCCTGTAGCcacaccc > 1:597884/1‑73 (MQ=255) cACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGGTTCTTACCGCCTTTAGGCACaaacc > 2:4561705/1‑73 (MQ=255) aCCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGGGGTTTCTCACTGCCTGTAGCCACCActct > 1:4002456/1‑76 (MQ=255) cccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCCCTGCCTGTAGCCACAACTCttt > 1:1468163/1‑75 (MQ=255) cTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCGGGTGCGCTTTTTCTCCCTGCCTTTAGCCACAACTCTTCAc < 2:1242775/76‑1 (MQ=255) cTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACTGCCTGTAGCCCCCACTCTTCaa > 2:4437220/1‑75 (MQ=255) tttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTTACTGCCTGTAGCCACCACTCTTCACtt > 1:59582/1‑76 (MQ=255) tttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTTTCTGCCTGTTGCCACAAATTTTCACtt > 1:1806408/1‑76 (MQ=255) ttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTATTACTTCCCGGTGCCACCACTCTTCACTtc > 2:1779194/1‑76 (MQ=255) ttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACCtc > 1:1110806/1‑76 (MQ=255) ttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGTTTCTCACTGCCCGTAGCCACAACACCTCACTtc > 1:1030949/1‑76 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCGGTTTTTCACTGCCCGTAACCACCACCCTTCACTtcc > 1:938743/1‑76 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGTGCTGTTTCTTACTCGCCGTAGCCACAACTCTTCACTtcc > 2:5325097/1‑76 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGGCCCAAACCTTCACTtcc > 2:3142721/1‑76 (MQ=255) gTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACTtccc > 1:5110689/1‑75 (MQ=255) gTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTtccc > 1:6814000/1‑75 (MQ=255) gTCTTGCGGGGGTCAATAATTGAGGGTGGCGGGTGCCTTTTTTCCCCCTGCTTGTAGCCACAACTCTTCACTtcct < 2:1976089/76‑1 (MQ=255) tCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAAATCTTCACTtcctc > 2:5429871/1‑76 (MQ=255) tCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTTCCTGTTGCCCCCACTcttcccttcccc > 2:6605848/1‑74 (MQ=255) cTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTTTCACTGCCTTTATTCACAAATCTTCACTtcctcc > 1:7087279/1‑76 (MQ=255) cTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTtcctcc > 1:4068478/1‑76 (MQ=255) cTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGGTTCTCACTGCCTGTAGCCACAACTCTTTACTttctcc > 2:6673557/1‑76 (MQ=255) cTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCCCAACTCTTCACTtcctcc > 1:5201984/1‑76 (MQ=255) tttCTGGCGTCGATCTTTGAGGCGGGCGGGTGCGCTTTTCCTCCCTGCCTTTAGCCACAACTCTTCACTTCCTccc < 2:1915468/76‑1 (MQ=255) tttCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACCGCCTGTAGCCACAACTCTTCACTTCCTccc > 2:6320200/1‑76 (MQ=255) ttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCt > 1:4704343/1‑76 (MQ=255) ttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCt > 2:801070/1‑76 (MQ=255) ttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCCGTAGCCACAACTCTTCACTTCCTCCCt > 2:4783122/1‑76 (MQ=255) ttCGGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCt < 1:2697677/76‑1 (MQ=255) tCTGGCGTCGATCATTGATGCTGGCAGGGGCGGTGTTTCTCACTGCCTGTATCCCCAAACCCTCACATTCTTCCtt > 1:3697790/1‑76 (MQ=255) cTGGCGTCGATCATTGATGCTGGCAGGTGCGCTTTTTCTCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCttt < 2:2142883/76‑1 (MQ=255) cTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCCCAACTCTTCACTTCCTCCCttt > 1:2243051/1‑76 (MQ=255) ggggTCGATCTTTGTTGCGGGCAGGTGCGCTGTTTCTCCCTGCTTTTAGCCACAACTCTTCACTTCCTCCCTTTAc < 1:4308950/73‑1 (MQ=255) ggCGTCGATCATTGATGCTGGCAGGGGGGCCGTTTTTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCttttc > 1:5226930/1‑74 (MQ=255) gCGTCGATCATTGATGCTGGCAGGTGCGCTTTTTCTCCCTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTAcc < 2:7003662/76‑1 (MQ=255) cGTCGATCATTGATGCTGGCAGGTGCGCTTTTTCTCCCTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCg < 1:5768610/76‑1 (MQ=255) tCGATCATTGATGCTGGCAGGTGCGCTTTTTCCCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGcc < 1:2931101/76‑1 (MQ=255) tCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGcc < 1:5293819/76‑1 (MQ=255) tCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTTCTCCCTTTTCcccc > 2:3651765/1‑76 (MQ=255) cGATCTTTGTTGCTGGCGGGTGCGTTTTTTCTCACTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCa < 2:2709126/76‑1 (MQ=255) cGATCATGGATGCTGGCGGGTGCTCTTTTTCCCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCa < 2:2391796/76‑1 (MQ=255) cATTGATGCTGGCAGGGGCGCCGTTTCTCACTGCCTGTAGGCACAAATCTTCACTTTCTCCCTTTACCGCCACTgg > 2:3862930/1‑76 (MQ=255) tttgttgCGGGCGGGTGCGTTTTTCCCCCCTGCTTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGa < 2:3285529/75‑1 (MQ=255) aTTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGa > 1:2337414/1‑76 (MQ=255) gATGCTGGCAGGGGCGGTGTTTTTCACCGCCTGTAGCCACCACTCTTCACTTCCTCCCCTTTCCGCCAGTTGAttt > 2:860804/1‑76 (MQ=255) aTGCGGGCGGGTGCTGTTTCTCCCATTCCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTg < 2:5536071/76‑1 (MQ=255) tGCTGGCAGGTGCGCTGTTTCTCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGc < 1:1665839/76‑1 (MQ=255) gCTGGCAGGGGCGCCGTTTCTCACTGCCTGTAGCCACAACTCCTCACTTCCTCCCTTTTTCGCCCGTGGGTTTGCt > 2:5253255/1‑76 (MQ=255) cTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTg > 2:1126722/1‑76 (MQ=255) tGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGa > 1:6209315/1‑76 (MQ=255) tGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGa > 1:1014689/1‑76 (MQ=255) cgggTGCTCTTTTTCTCCCTGCTTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGa < 1:6457812/74‑1 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 2:4986564/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 2:1305470/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGATGAAGAc > 2:506212/1‑76 (MQ=255) gTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCa > 1:3225621/1‑76 (MQ=255) tGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAg > 1:1165428/1‑76 (MQ=255) | GGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAG > REL606/4180174‑4180324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |