| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 414,056 | 0 | T | G | 37.7% | 273.2 / 41.4 | 138 | T345P (ACC→CCC) | yajR | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (10/76); new base G (52/0); total (62/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.04e-28 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 182 total aligned reads displayed.
CCCCCTGACCGTCAAACATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCTTC > REL606/413981‑414131 | cccccTGCCCGTAAAACATGCCGT‑CAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggt < 2:2057102/76‑1 (MQ=255)cccccTGACCGCAAAAAATGCCGT‑CAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggt < 2:3581467/76‑1 (MQ=255) cccTGACCGTCAAACATGCCGT‑CAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtgg < 1:1414769/76‑1 (MQ=255) ccTGACCGTCAAACATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtgggggg > 2:3546240/1‑75 (MQ=255) cTGCCCGACAACCATCCCGC‑CAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAg < 1:2973516/76‑1 (MQ=255) cTGACCGTCAAACATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggggggg > 2:4028756/1‑74 (MQ=255) tgcccGACAAACATCCCGT‑CAACCCACCCGCCCGGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGt < 2:6464831/73‑1 (MQ=255) ggcccGTCAACCATGCCGT‑CAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGt < 1:6005050/73‑1 (MQ=255) gACCGTCAAACATGCCGT‑CAATCCAGCCGCCCAGCGAAACGCCAATAGCCACGCCAAGAACCTGGCTGGGGGAgag > 2:5179860/1‑74 (MQ=255) ccttCAAACGCGCCGA‑CACCCCGGCCGCCCAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGa < 2:5717832/73‑1 (MQ=255) cGTCAAACATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGac < 1:2561943/76‑1 (MQ=255) cGTCAAACATCCCGT‑CAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGac < 1:6385025/76‑1 (MQ=255) cGTAAACCATGCCGC‑AAACCCACCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGac < 1:5619909/76‑1 (MQ=255) tcaaaaCATGCCGT‑CAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGaca < 1:2411519/73‑1 (MQ=255) gccAAACATGCCGT‑CAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGaca < 1:790425/74‑1 (MQ=255) gTCAAACATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGTAGaca > 1:4327742/1‑76 (MQ=255) tCAAACATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGacaa > 2:2792323/1‑75 (MQ=255) cAAACATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGTAGACAcc > 1:4144224/1‑76 (MQ=255) aaCATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGGGGTGACAcccc > 1:4752621/1‑75 (MQ=255) aaCATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGCGACAcccc > 1:5159707/1‑75 (MQ=255) aCATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACACCCac > 1:5271034/1‑75 (MQ=255) aCATCCCGT‑CACCCCGCCCGCCCAGCAACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCAt < 2:2595712/76‑1 (MQ=255) cATGCCCTCCAACCCGGCCCCCCAGCAACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCAt < 2:3611742/76‑1 (MQ=255) aTGCCGT‑CAACCCAGCCGCCCAGCAAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCg < 1:689086/76‑1 (MQ=255) tcccGT‑CAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGc < 1:2526060/74‑1 (MQ=255) tGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACACCCATCGc > 2:1703342/1‑76 (MQ=255) gCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAGAGACCCATcccc > 2:1066339/1‑76 (MQ=255) gCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGCGGGGGAGACACCCAACGcc > 1:7055813/1‑76 (MQ=255) cGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAGACACCCCTCGCCgc > 2:2233824/1‑75 (MQ=255) cGT‑CAACCCAGCCGCCCGGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGt < 1:1664725/76‑1 (MQ=255) gT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAGACACCCATCGCCgca > 2:6348559/1‑74 (MQ=255) cAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAGACACCCCTCGCCGCAcc > 2:5304250/1‑76 (MQ=255) cAACCCAGCCGCCCAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTAcc < 1:2875987/76‑1 (MQ=255) aacccAGCCCCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCt < 1:5961523/73‑1 (MQ=255) aaTCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACAACCCTCGCCCGACCt > 2:6332209/1‑76 (MQ=255) aaTCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAGACACCCATCGCCGTGCCt > 1:3242452/1‑76 (MQ=255) acccAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCtt < 2:4995202/74‑1 (MQ=255) cccAGCCGCCGAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCttt < 2:6869218/75‑1 (MQ=255) ccAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAGACAACCACGCCCGTACCCTTg > 2:3004778/1‑76 (MQ=255) cAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACACCCATCGCCGTACCTTTGt > 2:4583509/1‑76 (MQ=255) cAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACACCCACCGCCCTACCTTTGt > 1:5386939/1‑76 (MQ=255) aGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGTAGACAACCATCGCCGTACCTTTGTa > 2:509107/1‑76 (MQ=255) aGCCCCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTa < 1:4620277/76‑1 (MQ=255) gccgccCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAAACCCATCGCCGTACCCTTTTaa > 1:6432340/1‑76 (MQ=255) cgccCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACACCCCTCGCCGTACCTTTTTaaac > 2:6090704/1‑74 (MQ=255) cgccCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACACCCATCGCCGTACCCTTGTaaac > 2:4211799/1‑74 (MQ=255) cgccCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGCAGACACCCCTCGCCGTCCCCTTGTAAcc > 2:1222258/1‑76 (MQ=255) gccCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGGGTAGAAACCCATCGCCGTAACTTTGTAACct > 1:2115396/1‑76 (MQ=255) gccCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAGACACCCAACCCCGTACCCTTGTaaacc > 2:5862632/1‑73 (MQ=255) cccAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACCACCAACGCCGGAACTTTCTAAcccg > 2:5010246/1‑74 (MQ=255) cccAGCAACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACctg < 2:2654461/76‑1 (MQ=255) ccAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACACCCATCCCCGGACCTTTGTAAActgc > 1:2766263/1‑76 (MQ=255) aGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACACCCCTCGCCGTACCCTTGTAACctgctg > 2:234807/1‑76 (MQ=255) cgaaaCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTgg < 2:6771365/73‑1 (MQ=255) gAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCg < 2:7033382/76‑1 (MQ=255) aCCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGTAGACACCCATCGCCGGACCTTTGTAACCTGCTGGGGAc > 2:7145316/1‑76 (MQ=255) aCCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGGGGAGAAACCCCACGCCGTACCTTTGTAACCTGCTGGCggc > 2:2474300/1‑74 (MQ=255) ccAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCtt < 2:6753974/76‑1 (MQ=255) cAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt < 1:4087152/76‑1 (MQ=255) cAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt < 2:5753920/76‑1 (MQ=255) cAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt < 2:6442607/76‑1 (MQ=255) aaccgccacgcaaAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa < 2:111341/73‑1 (MQ=255) aaTCGCCACGCCAAGAAACTGGCTGGGGGGGGAGACACCCCTCGCCGTACCTTTGTAAACTGCTGGCGGCTCtttt > 2:3810486/1‑75 (MQ=255) cgccactccaAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTTCCTTTGTAACCTGCTGGCGACTCTTTACTg < 1:3581723/76‑1 (MQ=255) cgccacgccaAGAAACTGGCTGGGGGGGTAGACACCCATCGCCGTACCTTTGTAACCTTCTTGCGACTCTTTTCTg > 1:4005867/1‑76 (MQ=255) gccacgccaAGAAACTGGCTGGGGGGGGAGACACCCATCGCCGGACCTTTGTAACCTGCTGGCGGCTCTTTTCTGa > 1:6572842/1‑76 (MQ=255) ccacgccaAGAAACTGGCTGGTGGAGTAGACACCGATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt < 2:6409599/76‑1 (MQ=255) ccacgccaAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt < 2:3921940/76‑1 (MQ=255) ccacgccaAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt < 2:5524126/76‑1 (MQ=255) gccaAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt < 2:1952127/76‑1 (MQ=255) gccaAGAAACTGGCTGGGGGAGGAGAAACCCCTCACCGTACCATTGTTACCTGCTGGCGACTCCTTACTGATAAgg > 2:173346/1‑75 (MQ=255) ccaAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTg < 1:3827478/76‑1 (MQ=255) ccaAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTg < 1:4641167/76‑1 (MQ=255) aaGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAg < 1:1002631/76‑1 (MQ=255) aaGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAg < 1:1002315/76‑1 (MQ=255) aaGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAg < 1:1574095/76‑1 (MQ=255) gAAACTGGCTGGGGGGGGAGACACCCCTCGCCGTACCTTTGTAACCTGCTGGCGGCTCTTTTCTGGTAAGTGAggg > 2:3052850/1‑76 (MQ=255) gAAACTGGCTGGGGGGGGAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAggg > 2:6914043/1‑76 (MQ=255) aagctggctggTGGCGTAGACCCCCACCGCCGTACCTTTGTACCCTGCTGGCGACTCTTTACTGATAAGTGAGGGc < 2:2097371/73‑1 (MQ=255) aaaCTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGc < 1:1996163/76‑1 (MQ=255) aaaCTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGc < 2:6576229/76‑1 (MQ=255) aaaCTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGc < 1:3109582/76‑1 (MQ=255) aaCTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCa < 2:1461072/76‑1 (MQ=255) aaCTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCa > 1:5944888/1‑76 (MQ=255) aaCTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCa > 1:2257163/1‑76 (MQ=255) aCTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAg < 1:1357363/76‑1 (MQ=255) aCTGGCGGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAg < 2:5707239/76‑1 (MQ=255) ctggctggTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGa > 1:2658274/1‑76 (MQ=255) tggctggTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGaa < 2:3448926/76‑1 (MQ=255) tggctggTGGAGGAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGaa > 2:1307560/1‑76 (MQ=255) ggctggTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAg < 1:4246789/76‑1 (MQ=255) ggctggTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAg < 1:4688897/76‑1 (MQ=255) ggctggTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAg < 1:6287747/76‑1 (MQ=255) ctggTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAggg > 1:3085170/1‑76 (MQ=255) ctggTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAggg > 1:5606371/1‑76 (MQ=255) ggtggAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCt < 2:4698731/76‑1 (MQ=255) gtggAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCtt < 2:1615304/76‑1 (MQ=255) gtggAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCtt > 2:448754/1‑76 (MQ=255) tggAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCTTc < 1:5492433/76‑1 (MQ=255) tggAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCTTc < 1:1088276/76‑1 (MQ=255) | CCCCCTGACCGTCAAACATGCCGT‑CAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAGACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGAAGGGCTTC > REL606/413981‑414131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |