| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 476,746 | 0 | T | G | 45.7% | 122.7 / 33.4 | 105 | Q6H (CAA→CAC) | fsr | predicted fosmidomycin efflux system |
| Reads supporting (aligned to +/- strand): ref base T (38/19); new base G (48/0); total (86/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 159 total aligned reads displayed.
TGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTAC > REL606/476673‑476821 | tGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGgttg > 2:1769919/1‑76 (MQ=255)tGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGggtg > 2:273338/1‑76 (MQ=255)tGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGggtg > 1:6005274/1‑76 (MQ=255) gATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGgttgt > 2:7160145/1‑76 (MQ=255) gATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGggtgt > 2:6872329/1‑76 (MQ=255) aGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCa > 2:409483/1‑76 (MQ=255) gCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCAc > 2:3262325/1‑76 (MQ=255) ccTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCa > 1:950618/1‑76 (MQ=255) cTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGGGTTCACTCAt > 2:4717764/1‑76 (MQ=255) aaaaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTCCTCAttt > 2:5585775/1‑75 (MQ=255) aaaaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTg > 2:6806059/1‑76 (MQ=255) aaaTACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGc > 2:3539759/1‑76 (MQ=255) aCCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACACAATGGCATa > 1:1255021/1‑76 (MQ=255) ccAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTCCTCCTTGCCCTAg > 1:6148766/1‑76 (MQ=255) ccAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGCCATAg > 1:6291844/1‑76 (MQ=255) ccAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCCTAg > 1:1672705/1‑76 (MQ=255) ccAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAg > 1:6761162/1‑76 (MQ=255) ccAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAg > 2:4538320/1‑76 (MQ=255) agaCGTTGTGCGGGCGTTGGGCGGCGCCGCCCCCCCCCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGa < 1:2284580/74‑1 (MQ=255) aaCGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGaa > 2:2387219/1‑76 (MQ=255) cGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAg > 2:3532236/1‑76 (MQ=255) cGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGCCCTAGGAAAg > 2:3725273/1‑76 (MQ=255) cGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAg > 1:5664709/1‑76 (MQ=255) aTGTTTGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCCTTTCCATAGGAAAAT‑c > 2:5741137/1‑76 (MQ=255) aTGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑c > 2:5303266/1‑76 (MQ=255) aTGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGCCATAGGGAAggc > 1:395813/1‑74 (MQ=255) aTGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCCTTGCCCTAGGGAAGT‑c > 2:1441550/1‑76 (MQ=255) aTGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGgaaata > 2:4796218/1‑73 (MQ=255) gTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCCCTCATTGTCATAGGGAAGT‑CAc > 1:2061880/1‑76 (MQ=255) cGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTCCTCCTTTCCATAGGAAAGG‑CACttt > 2:2321108/1‑76 (MQ=255) cGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCCTTGCCATAGGGAAGG‑CCCttt > 1:2360263/1‑76 (MQ=255) cGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGGAAAG‑CACttt > 2:534805/1‑76 (MQ=255) cGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACttt > 1:3508560/1‑76 (MQ=255) cGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACttt > 1:2587894/1‑76 (MQ=255) cGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACCCATTGCCATAGGAAAGG‑CACttt > 2:570235/1‑76 (MQ=255) gggCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTTACTCATTGCCATAGGAAAGG‑CACtttt > 1:1798001/1‑76 (MQ=255) gggCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCCTAGGAAAGT‑CACtttt > 1:5770575/1‑76 (MQ=255) ggCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTTCCCTAGGGAAGT‑CAActttt > 1:2109033/1‑76 (MQ=255) ccTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑AGCtttttta > 1:3583556/1‑74 (MQ=255) cTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTTACTCATTGCCATAGGGAAGT‑CACTTTTTTAg > 2:609542/1‑76 (MQ=255) cTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTTCCAGAAGGAAGA‑TACTTTTTTAg > 2:1991171/1‑76 (MQ=255) cTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGGAAGT‑CACTTTTTCAg > 1:2622153/1‑76 (MQ=255) tGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGCCATAGGAAAGT‑CACTTTTTCAggg > 1:285670/1‑76 (MQ=255) tGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGCCATAGGAAAGG‑CCCTTTTTCAggg > 1:6183811/1‑76 (MQ=255) tGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAggg > 2:4526676/1‑76 (MQ=255) gTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCCTTGCCCTAGGAAAGTACATTTTTTCgggtg > 2:2200958/1‑74 (MQ=255) tCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTTCTTGCCATAGGAAAGT‑CACTTTTTTAGGGGTg > 1:3522599/1‑76 (MQ=255) tCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTg > 1:7063772/1‑76 (MQ=255) gTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGGAAGT‑CACTTTTTCAGGGGTGCg > 2:6072421/1‑76 (MQ=255) gTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGGTGCg > 1:1928329/1‑76 (MQ=255) ttGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGa > 2:2602573/1‑76 (MQ=255) gACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTTACTCATTGGCATAGGAAAAT‑CACTTTTTCCGGGTTGCGATg > 2:2706835/1‑76 (MQ=255) gACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATg > 2:1351808/1‑76 (MQ=255) cGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTTCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTa > 2:207727/1‑76 (MQ=255) cGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGGTGCGATGTa > 2:6236210/1‑76 (MQ=255) cAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGGTGCGATGTaaa > 2:5603226/1‑76 (MQ=255) aGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGGCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTGAAg > 2:6933326/1‑76 (MQ=255) aGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAg > 2:4682043/1‑76 (MQ=255) aGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACCTTTTCAGGGGTGCGATGTaaaa > 2:4275016/1‑75 (MQ=255) gccgccCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGa < 1:80342/76‑1 (MQ=255) ccGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGG‑CACTTTTTCAGGGTTGCGATGTAAAGaa > 1:1978385/1‑76 (MQ=255) ccGCCCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGaa < 1:3373136/76‑1 (MQ=255) cgcgCCCGCCACAGGCTGGGGTTGTTTACTCAATGCCTTAGGAAAGT‑CACTTTTTCAGGGTTGCGCAGTAAAGAAt > 2:5865985/1‑76 (MQ=255) cgcgCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAAt > 2:1442327/1‑76 (MQ=255) cgcgCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAAt > 2:1620350/1‑76 (MQ=255) cgcgCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGGTGCGATGTAAAGAAt > 2:4277895/1‑76 (MQ=255) gcgcCCGCCACAGGCTGGGGTTGTTCACTCATTGCCCTAGGAAAAT‑CACTTTTTCAGGGTTACGATGTAAAGAATg > 1:1231586/1‑76 (MQ=255) cgcccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGa > 1:1718696/1‑76 (MQ=255) cgcccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGa > 2:7073164/1‑76 (MQ=255) cgcccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGa > 1:2374488/1‑76 (MQ=255) cgcccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGa > 1:4928170/1‑76 (MQ=255) cgcccgccACAGGCTGGGGGTGTTTACTCATTGCCATAGGAAAGT‑CCCTTTTTCAGGGTTGCGATGTAAAGAATGa > 2:3111483/1‑76 (MQ=255) cgcccgccACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGa > 2:5799389/1‑76 (MQ=255) cgcccgccACAGGCTGGGGGTGTTCACTCATTGCCATAGGAAAGA‑CACTTTTTCCGGGTTGCGATGTAAAGAATGa > 1:4867223/1‑76 (MQ=255) cccccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGa < 1:3690569/74‑1 (MQ=255) cccccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGa < 2:5431036/74‑1 (MQ=255) gcccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGAt > 1:1574572/1‑76 (MQ=255) cccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATc > 2:3883539/1‑76 (MQ=255) ccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCt < 2:2622614/76‑1 (MQ=255) ccgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAAAATGATCt > 1:3375026/1‑76 (MQ=255) cgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCtt < 2:5089704/76‑1 (MQ=255) cgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCtt < 2:5478109/76‑1 (MQ=255) gccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTa < 2:4359235/76‑1 (MQ=255) gccACAGGCTGGGGGTGTTCAACCCTTGCCTTAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTGAAGAATGATCTTa > 2:6057790/1‑76 (MQ=255) acaGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTAttt < 1:5868130/76‑1 (MQ=255) caGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtg < 2:1258639/76‑1 (MQ=255) caGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtg > 2:6549227/1‑76 (MQ=255) ggCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtgtg < 2:3653407/76‑1 (MQ=255) ggCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtgtg < 1:818606/76‑1 (MQ=255) ggCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtgtg > 2:5917326/1‑76 (MQ=255) ggCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtgtg < 1:1475527/76‑1 (MQ=255) ggCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtgtg < 1:51835/76‑1 (MQ=255) ggCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTtgtg < 1:5635111/76‑1 (MQ=255) cTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGat > 1:6463336/1‑76 (MQ=255) tGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatt > 1:3570032/1‑76 (MQ=255) ggggTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGatta < 2:4097603/76‑1 (MQ=255) gggTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGattat > 2:1527187/1‑76 (MQ=255) gggTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGattat > 1:3918342/1‑76 (MQ=255) gttgttCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGAttatta > 2:2979133/1‑76 (MQ=255) gttgttCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGAttatta > 1:6597915/1‑76 (MQ=255) | TGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGT‑CACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTAC > REL606/476673‑476821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |