| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,498 | C→A | A270S (GCC→TCC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,498 | 0 | C | A | 99.5% | 676.6 / NA | 186 | A270S (GCC→TCC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (87/98); minor base G (1/0); total (88/98) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 213 total aligned reads displayed.
TCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAA > REL606/474425‑474570 | tCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAAc < 1:5276965/76‑1 (MQ=255)tCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAAc < 2:6426197/76‑1 (MQ=255) aaaCAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACgg < 2:1602282/76‑1 (MQ=255) aaaCAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACgg < 2:3292404/76‑1 (MQ=255) aaaCAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACgg < 2:6684647/76‑1 (MQ=255) aaaCAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACgg > 1:2958525/1‑76 (MQ=255) aaaCAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGGATCGTGGGCGGAACgg > 1:6440046/1‑76 (MQ=255) aacaacATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGt > 1:211819/1‑76 (MQ=255) acaacaTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTg < 2:3106340/76‑1 (MQ=255) caacaTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGa > 2:4443162/1‑76 (MQ=255) aacaTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGGGAc > 2:4911476/1‑76 (MQ=255) acaTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACt < 2:532485/76‑1 (MQ=255) caTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGaaca > 2:5420836/1‑73 (MQ=255) caTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGGGACTc > 1:5424161/1‑76 (MQ=255) aTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCa > 1:6154588/1‑76 (MQ=255) tCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAg > 1:660219/1‑76 (MQ=255) tCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAg > 1:2236171/1‑76 (MQ=255) tCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAg > 2:6629049/1‑76 (MQ=255) tCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGAATCAg > 1:3032824/1‑76 (MQ=255) ccGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGtt > 1:5929332/1‑76 (MQ=255) cGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTc > 1:1811619/1‑76 (MQ=255) cGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTc > 2:3967635/1‑76 (MQ=255) gACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCa > 1:2977516/1‑76 (MQ=255) gACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCa < 1:3095807/76‑1 (MQ=255) aCGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAg > 2:2007517/1‑76 (MQ=255) aCGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAg < 1:3807908/76‑1 (MQ=255) aCGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAg < 1:6961033/76‑1 (MQ=255) aCGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAg < 1:776734/76‑1 (MQ=255) tgagaCAAAAAGCAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGAc > 2:297268/2‑76 (MQ=255) ggAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGAc > 2:4428670/1‑76 (MQ=255) gagaCAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACt < 2:5516322/76‑1 (MQ=255) gagaCAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACt > 2:4745562/1‑76 (MQ=255) agaCAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTc > 1:4427297/1‑76 (MQ=255) agaCAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTc > 1:3413534/1‑76 (MQ=255) agaCAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTc < 1:2642080/76‑1 (MQ=255) cAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGtt > 1:1371798/1‑76 (MQ=255) cAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGtt < 2:1434595/76‑1 (MQ=255) cAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGtt > 2:2898562/1‑76 (MQ=255) aaaaaaCAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGGGACTCAGTTCAGACTCGTTc > 1:4069577/1‑76 (MQ=255) aaaaCAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAg < 1:4930914/76‑1 (MQ=255) aaCAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGGTCCGACTCGTTCAGTa > 2:1200437/1‑76 (MQ=255) cAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTAcc > 2:3531693/1‑76 (MQ=255) cAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTAcc < 1:7076521/76‑1 (MQ=255) cAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTAcc < 2:5698280/76‑1 (MQ=255) aGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCa < 1:5374031/76‑1 (MQ=255) gCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTAccct > 2:2127973/1‑74 (MQ=255) gCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCAt < 2:4556465/76‑1 (MQ=255) aCCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATcc > 2:3771697/1‑76 (MQ=255) ccGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATccc > 2:2235996/1‑76 (MQ=255) gCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCgg > 1:321349/1‑76 (MQ=255) cAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGAGTCGTTTCGTACCATCCCgga > 2:4185455/1‑75 (MQ=255) cAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGAGTCGTTCAGTACCATCCCGGc > 2:3122820/1‑76 (MQ=255) cAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGc > 1:1868746/1‑76 (MQ=255) cAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGc > 2:4502228/1‑76 (MQ=255) aaaCGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCa > 1:1410401/1‑76 (MQ=255) aaaCGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGGTCAGTACCATCCCGGCa > 2:2800374/1‑76 (MQ=255) aaCGCGTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCCGACTCGTTCAGTACCATCCCGGcga > 1:513101/1‑74 (MQ=255) cgcgTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAg > 1:1226092/1‑76 (MQ=255) cgcgTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAg < 2:3414969/76‑1 (MQ=255) cgcgTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAg > 2:196357/1‑76 (MQ=255) gcgTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGa < 2:5066458/76‑1 (MQ=255) cgTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGaa < 2:2732143/76‑1 (MQ=255) cgTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGaa < 2:6765617/76‑1 (MQ=255) gTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGaaa > 1:521073/1‑75 (MQ=255) gTCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAAc > 1:5726659/1‑76 (MQ=255) tCGCGCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCCGTACCATCCCGGCAAAGAAcc > 2:128759/1‑75 (MQ=255) cgcgCAATGGCAGCGTATCGTGGGCGGAGCGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGc > 1:1740288/1‑76 (MQ=255) cgcgCAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGc > 1:2550580/1‑76 (MQ=255) cAATGGCAGCGTATTGGGGGCGGAACGGGGACTCAGTTCAGACCCGTTCAATAACATCCCGGCAAAGACAGCACCg > 2:5762948/1‑76 (MQ=255) cAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCg > 2:3627391/1‑76 (MQ=255) cAATGGCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCg > 2:861467/1‑76 (MQ=255) aTGCCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGGTCAGGACCATCCCGGCAAAGAACGCACCgcg > 1:1045618/1‑74 (MQ=255) ggCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTg < 1:2414202/76‑1 (MQ=255) ggCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTg > 1:5656326/1‑76 (MQ=255) gCAGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTGCCATCCCGGCAAAGAACGCACCGAGTGc < 1:4984998/76‑1 (MQ=255) aGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCaa < 2:2890925/76‑1 (MQ=255) aGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCaa < 2:7058392/76‑1 (MQ=255) aGCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCaa < 1:5973653/76‑1 (MQ=255) gCGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCaaa < 2:1811619/76‑1 (MQ=255) cGTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAg > 1:6207933/1‑76 (MQ=255) gTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAgg < 1:2077614/76‑1 (MQ=255) gTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAgg > 1:912409/1‑76 (MQ=255) gTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAgg > 1:2300699/1‑76 (MQ=255) gTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAgg < 1:4103843/76‑1 (MQ=255) gTATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAgg < 1:2826645/76‑1 (MQ=255) tATCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGga > 2:4391145/1‑76 (MQ=255) aTCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGgag > 1:3845376/1‑76 (MQ=255) tCGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGgaga < 2:5339327/76‑1 (MQ=255) cgggggCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGAc < 1:4668874/73‑1 (MQ=255) cGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGAc < 1:3677830/76‑1 (MQ=255) cGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGAc < 2:6010662/76‑1 (MQ=255) cGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGAc < 1:2082932/76‑1 (MQ=255) cGTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGAc > 1:5040689/1‑76 (MQ=255) gTGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACa > 2:1780892/1‑76 (MQ=255) tGGGCGGAACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACAt > 2:1152859/1‑76 (MQ=255) ggcggaACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCa < 1:3516194/76‑1 (MQ=255) ggcggaACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCa > 2:6846705/1‑76 (MQ=255) ggcggaACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCa > 2:3747188/1‑76 (MQ=255) gcggaACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCaa < 1:1555160/76‑1 (MQ=255) cggaACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCaaa > 2:7194387/1‑76 (MQ=255) | TCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAA > REL606/474425‑474570 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |