| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,044,167 | A→G | L28P (CTG→CCG) | rfbA ← | glucose‑1‑phosphate thymidylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,044,167 | 0 | A | G | 99.4% | 694.6 / ‑4.0 | 167 | L28P (CTG→CCG) | rfbA | glucose‑1‑phosphate thymidylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (70/96); total (71/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01 | |||||||||||
Only 100 of 196 total aligned reads displayed.
AATATCGCGAATACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCAGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCTTTAC > REL606/2044094‑2044239 | tatCGCGAATACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGgttg > 1:5387452/1‑76 (MQ=255) atCGCGAATACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGgttgt > 1:296993/1‑76 (MQ=255) atCGCGAATACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGgttgt > 1:2082245/1‑76 (MQ=255) atCGCGAATACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGgttgt > 1:6960830/1‑76 (MQ=255) gcgAATACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTtta < 1:775141/76‑1 (MQ=255) gAATACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTttact > 1:6325064/1‑76 (MQ=255) gAATACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTttact > 2:3356405/1‑76 (MQ=255) gAATACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTttact < 1:5630522/76‑1 (MQ=255) aaTACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTttactt < 2:4352656/76‑1 (MQ=255) aTACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTttactta < 1:124216/76‑1 (MQ=255) aCCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGGTGTTtaatttct > 2:1502994/1‑76 (MQ=255) cccGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGTGGTTGTTtacttactc > 2:6643906/1‑75 (MQ=255) cccGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTttacttatca < 2:6457612/76‑4 (MQ=255) cccGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTttcttactt > 2:240043/1‑75 (MQ=255) cccGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTg < 2:2357678/76‑1 (MQ=255) ccGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGGTTGATTTCTGc > 2:4570988/1‑76 (MQ=255) cGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTggc > 1:5824540/1‑74 (MQ=255) cGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGcc > 1:5410634/1‑76 (MQ=255) cGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGcc > 1:3345031/1‑76 (MQ=255) cGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGcc < 1:3379448/76‑1 (MQ=255) cGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGcc > 1:6040223/1‑76 (MQ=255) gCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCa < 2:509857/76‑1 (MQ=255) gCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCa > 1:6817057/1‑76 (MQ=255) cTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCAt > 1:2844854/1‑76 (MQ=255) tAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATc < 2:3299400/76‑1 (MQ=255) tAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATc > 1:4265595/1‑76 (MQ=255) tAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATc < 2:2512639/76‑1 (MQ=255) tAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATc < 1:1602729/76‑1 (MQ=255) aaCATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTTCTGCCATCg > 2:2466332/1‑76 (MQ=255) aaCATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTTCCATCg > 2:7091444/1‑76 (MQ=255) aaCATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCg > 1:7122484/1‑76 (MQ=255) aaCATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCg > 1:120775/1‑76 (MQ=255) aaCATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGGTGTTTACTTTCTGCCATCg > 2:3850943/1‑76 (MQ=255) aCATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGt > 1:5956742/1‑76 (MQ=255) cATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCCTCGTc > 2:3541213/1‑76 (MQ=255) aTAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTca < 1:3934090/76‑1 (MQ=255) aGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAg < 1:2878730/76‑1 (MQ=255) aGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAg < 1:6200731/76‑1 (MQ=255) gCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAgg < 1:999036/76‑1 (MQ=255) gCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAgg < 1:4117576/76‑1 (MQ=255) gTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGAt < 1:4677064/76‑1 (MQ=255) ttGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATa < 2:3480676/76‑1 (MQ=255) ttGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATa < 1:6840141/76‑1 (MQ=255) gAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATaaa < 1:4299993/76‑1 (MQ=255) aaaGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATaaaa > 2:5812762/1‑75 (MQ=255) aaaGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAg > 1:3644568/1‑76 (MQ=255) aaaGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAg < 1:1725183/76‑1 (MQ=255) aaaGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAg > 1:2438143/1‑76 (MQ=255) aaGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGa < 1:2796720/76‑1 (MQ=255) aGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGAc < 2:6208835/76‑1 (MQ=255) gCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACg > 1:1335554/1‑76 (MQ=255) gCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACg < 1:3189531/76‑1 (MQ=255) ataataAATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTg < 2:5122828/76‑1 (MQ=255) ataaATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCg < 2:5953044/76‑1 (MQ=255) taaATCATCGGCTTATCATAAATCGGCAGCGGTTGTTTACTTGCTGCCATCGTCACAGGATAAAGACGAGTGCCgg < 1:6980125/76‑1 (MQ=255) atcatcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTTCTGCCATCGTCACAGGATAAAGACGGGTGCCGGCac > 1:2256416/1‑76 (MQ=255) atcatcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAac < 2:3272362/76‑1 (MQ=255) atcatcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAac > 1:5211984/1‑76 (MQ=255) atcatcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAac > 1:5002906/1‑76 (MQ=255) atcatcGGCTTATCATAAATCGGCAGCGGGTGTTTACTTACTGCCATCGTCACAGGATAAAAACGAGGGGCGGGac > 2:1997915/1‑76 (MQ=255) tcatcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCCTCGTCACAGGATAAAGACGAGTGCCGGaaac > 2:5231866/1‑74 (MQ=255) tcatcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAacc < 2:2971344/76‑1 (MQ=255) tcatcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAacc > 2:2578832/1‑76 (MQ=255) tcatcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAacc < 2:844633/76‑1 (MQ=255) tcatcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAacc < 1:4570462/76‑1 (MQ=255) acatcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACAGCCATCGTCACAGGATAAAGACGAGTGCCGGAacc < 2:6678293/75‑1 (MQ=255) catcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAacca < 2:4137877/76‑1 (MQ=255) tcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAaccacc < 2:2744012/76‑1 (MQ=255) tcGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAaccacc < 2:6135982/76‑1 (MQ=255) cGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAAccacca < 2:4705684/76‑1 (MQ=255) cGGCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAAccacca > 1:3719838/1‑76 (MQ=255) ggCTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCAccag < 2:6928358/76‑1 (MQ=255) cTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCAccagcc < 2:1891448/76‑1 (MQ=255) cTTATCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCAccagcc < 2:1609604/76‑1 (MQ=255) tCATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaa > 1:5321446/1‑76 (MQ=255) cATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaat > 1:3597870/1‑76 (MQ=255) cATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaat > 1:6639102/1‑76 (MQ=255) cATAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaat > 1:5141503/1‑76 (MQ=255) aaaaaTCGGCAGCGGTTGTTTCCTTGCTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaata < 2:4103921/74‑1 (MQ=255) aTAAATCGGCAGCGTTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaata < 1:5569339/76‑1 (MQ=255) aTAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaata < 1:4486042/76‑1 (MQ=255) tAAATCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaataa < 2:3812308/76‑1 (MQ=255) aaaTCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGTATAAAGACGAGTGCCGGAACCACCAGCCAGaataat > 1:5663623/1‑76 (MQ=255) aaaTCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaataat < 1:2415571/76‑1 (MQ=255) aaaTCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaataat > 1:4935232/1‑76 (MQ=255) aaaTCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGaataat < 1:7106908/76‑1 (MQ=255) aaTCGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCCAAataata > 2:4795273/1‑76 (MQ=255) cGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCt > 2:1953963/1‑76 (MQ=255) cGGCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCt > 1:429118/1‑76 (MQ=255) ggCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCtt > 2:5482462/1‑76 (MQ=255) ggCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCtt < 2:3904462/76‑1 (MQ=255) ggCAGCGGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCtt < 2:2934094/76‑1 (MQ=255) gcagcgGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCttt < 2:516447/76‑1 (MQ=255) gcagcgGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCttt > 2:4436097/1‑76 (MQ=255) gcagcgGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCttt > 1:7013509/1‑76 (MQ=255) gcagcgGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCttt < 1:1984059/76‑1 (MQ=255) gcagcgGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCttt < 1:1111509/76‑1 (MQ=255) cagcggTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCTTTa > 1:5738633/1‑76 (MQ=255) cagcggTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCTTTa > 1:2658098/1‑76 (MQ=255) cagcggTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCTTTa < 1:1031103/76‑1 (MQ=255) | AATATCGCGAATACCCGCTAACATAAGCGTTGAAAGCGGATAATAAATCATCGGCTTATCATAAATCGGCAGCAGTTGTTTACTTACTGCCATCGTCACAGGATAAAGACGAGTGCCGGAACCACCAGCCAGAATAATACCTTTAC > REL606/2044094‑2044239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |