BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	717.2 / NA	181	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (68/113); total (68/113)

Only 100 of 221 total aligned reads displayed.

AGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193705‑2193851

  ccGATGCATCCGGCGTCCAGGCGCGGCTGGGGT‑GGATGGCGGGAAATCCGCCCCAAAAACAAAAAAACG‑CAAAAcc                                                                        >  1:2052285/1‑76 (MQ=255)

   cGATGCATCCGGCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATcccc                                                                      <  1:3150101/76‑1 (MQ=255)

    gATGCATCCGGCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCcca                                                                     <  2:4309575/76‑1 (MQ=255)

     aTGCATCCGGCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCccac                                                                    <  1:1148452/76‑1 (MQ=255)

     aTGCATCCGGCGTCCAGGCGCGGCTGGGCT‑GGCTGTC‑GGGAAACAGCCCCCAGAAAAACAAAAAGACCAACccccc                                                                     >  1:6124197/1‑75 (MQ=255)

      tGCATCCGGCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCccacc                                                                   <  2:145542/76‑1 (MQ=255)

      tGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGCGGTG‑GGAAAT‑AACCCCCATAACACCAAAAAG‑CCCAACCccacc                                                                   >  2:3369443/1‑76 (MQ=255)

      tGCATCCGGCGTCCAGGCGCGGCTGGGCT‑GGCTGTC‑GGGAATCAGCCCCAAAAAAAAGAAAAAC‑CCAACCCccacc                                                                   >  1:2718948/1‑76 (MQ=255)

      tGCATCCGGCGTCCAGGCGCGGCTGGGCT‑GGCTGTC‑GGGAAACAGCCCCAATAACAGCAAAACG‑CCAAACCccacc                                                                   >  1:6525062/1‑76 (MQ=255)

       gCATCCGGCGTCCAGGCGCGGCTGGTTT‑GGGTGTC‑GGGAATTAACCCCCAAAACAACAAAAAG‑CCCATCccccccc                                                                  >  1:883529/1‑75 (MQ=255)

       gCATCCGGCGTCCAGGCGCGGCTGGGCT‑GGGTGTC‑CGAAATTAGCCCCAAACACAGCCAAACG‑CCAAACCccacca                                                                  >  1:3390477/1‑76 (MQ=255)

       gCATCCGGCGTCCAGGCGCGGCTGGGCT‑GGCTGTC‑GGGAATTAGGCCCCATAACAACAAAAAC‑CCAAACCccacca                                                                  >  2:1235322/1‑76 (MQ=255)

        cATCCGGCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAg                                                                 <  1:1748990/76‑1 (MQ=255)

        cATCCGGCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAg                                                                 <  1:5670133/76‑1 (MQ=255)

        cATCCGGCGTCCAGGCGCGGCTGGGCT‑GGGTGTC‑GGGAATCAAACCCCATAAACACAAAAAC‑CCAAACAccaccac                                                                 >  2:4158713/1‑75 (MQ=255)

        cATCCGGCGTCCAGGCGCGGCTGGGCT‑CGGTTTCGGGGAAAAAGCCCC‑ATAAAAACCAAAAG‑CCAATCccccccca                                                                 >  2:4869072/1‑74 (MQ=255)

         aTCCGGCGTCCAGGCGCGGCTGGGCT‑GGCTGTC‑GGGAATCAGGCCCAAAAAAACGAAAACG‑CCAATCCcccccaca                                                                >  2:1536220/1‑74 (MQ=255)

         aTCCGGCGTCCAGGCGCGGCTGGGCT‑GGCTGTC‑GGGAAACAGCCCCCATAACAACCAAACC‑CCCAACcccccccca                                                                >  2:2435847/1‑73 (MQ=255)

          tCCGGCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGAt                                                               <  1:4129411/76‑1 (MQ=255)

           ccGGCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGGAAACAGCCCCCATAAAAACAAAACG‑CCCATCCCCACCCGATa                                                              >  2:471462/1‑76 (MQ=255)

           ccGGCGTCCAGGCGCGGCTGGGCT‑GGCTGTCGGGAAACCAG‑CCCAATAACAGCAAAAAA‑CCAATCCCCACCCGATa                                                              >  2:5665188/1‑76 (MQ=255)

           ccGGCGTCCAGGCGCGGCTGGGCT‑GGCTGTC‑GGGAAACAGCCCCCAAAACAACAAAAAG‑CCAAACCCCCCCCGATa                                                              >  2:5992679/1‑76 (MQ=255)

            cggcggCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATaa                                                             <  2:7198689/76‑1 (MQ=255)

             ggCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATaaa                                                            <  1:2315466/76‑1 (MQ=255)

             ggCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATaaa                                                            <  2:1096635/76‑1 (MQ=255)

             ggCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATaaa                                                            <  2:2089696/76‑1 (MQ=255)

             ggCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATaaa                                                            <  2:4265301/76‑1 (MQ=255)

              gCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAg                                                           <  2:5858439/76‑1 (MQ=255)

               cGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGc                                                          <  1:2748114/76‑1 (MQ=255)

               cGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGc                                                          <  2:3981846/76‑1 (MQ=255)

               cGTCCAGGCGCGGCTGGGCT‑GGCTGTC‑GGAAAACAGCCCCCATAACAGCAAAACG‑CCAAACCCCACCACATaaaac                                                          >  1:5782617/1‑74 (MQ=255)

                gTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCa                                                         <  1:1636983/76‑1 (MQ=255)

                 tCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCAt                                                        <  2:5222696/76‑1 (MQ=255)

                   cAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGa                                                      <  1:1716488/76‑1 (MQ=255)

                   cAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGa                                                      <  1:6755731/76‑1 (MQ=255)

                        gcgGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCt                                                 <  2:456198/76‑1 (MQ=255)

                        gcgGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCt                                                 <  2:3872058/76‑1 (MQ=255)

                         cgGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:5720680/76‑1 (MQ=255)

                          ggCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGt                                               >  2:1742435/1‑76 (MQ=255)

                            cTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  2:7034843/1‑76 (MQ=255)

                             tGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:2394702/76‑1 (MQ=255)

                              ggTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  1:6397139/1‑76 (MQ=255)

                               gTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          >  1:3503264/1‑76 (MQ=255)

                               gTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          >  2:3153839/1‑76 (MQ=255)

                               gTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          >  2:5285107/1‑76 (MQ=255)

                               gTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:2275551/76‑1 (MQ=255)

                                 cT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:3559881/1‑76 (MQ=255)

                                 cT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:1176130/76‑1 (MQ=255)

                                    ggCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  2:3195107/76‑1 (MQ=255)

                                     gCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     >  2:499538/1‑76 (MQ=255)

                                      cTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  1:5450518/1‑76 (MQ=255)

                                       tGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   <  2:6549035/76‑1 (MQ=255)

                                        gTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  1:6456267/1‑76 (MQ=255)

                                        gTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:6313320/76‑1 (MQ=255)

                                         tC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:2722658/76‑1 (MQ=255)

                                         tC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 >  2:621714/1‑76 (MQ=255)

                                          c‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  1:1049088/76‑1 (MQ=255)

                                            ggAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:5147019/76‑1 (MQ=255)

                                             gAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              >  1:1184466/1‑76 (MQ=255)

                                             gAAATCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:3471138/76‑1 (MQ=255)

                                                aTCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           >  2:6414466/1‑76 (MQ=255)

                                                aTCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  1:5890027/76‑1 (MQ=255)

                                                 tCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          <  1:1381242/76‑1 (MQ=255)

                                                 tCAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          <  2:5451154/76‑1 (MQ=255)

                                                  cAGCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:2595229/76‑1 (MQ=255)

                                                    gCCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  1:3748916/76‑1 (MQ=255)

                                                     ccccAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      >  2:6315657/1‑76 (MQ=255)

                                                      cccAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATggtg                     <  2:410532/76‑3 (MQ=255)

                                                      cccAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATggtg                     <  2:780262/76‑3 (MQ=255)

                                                      cccAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATggtg                     <  2:3611020/76‑3 (MQ=255)

                                                      cccAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATggtg                     <  2:2616082/76‑3 (MQ=255)

                                                       ccAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  1:5951496/1‑76 (MQ=255)

                                                       ccAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  1:2485393/1‑76 (MQ=255)

                                                        cAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   <  2:6721650/76‑1 (MQ=255)

                                                         aaTAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  1:2190815/1‑76 (MQ=255)

                                                           tAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  2:1916755/1‑76 (MQ=255)

                                                            aaCAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  1:6209503/1‑76 (MQ=255)

                                                            aaCAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               <  2:5474831/76‑1 (MQ=255)

                                                              cAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             >  2:3795061/1‑76 (MQ=255)

                                                              cAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             >  1:5286105/1‑76 (MQ=255)

                                                                gCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  2:679846/76‑1 (MQ=255)

                                                                gCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  2:1074886/76‑1 (MQ=255)

                                                                 cAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc          <  2:3906193/76‑1 (MQ=255)

                                                                  aaaaCG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  1:3294302/76‑1 (MQ=255)

                                                                   aaaCG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        >  1:7034438/1‑76 (MQ=255)

                                                                    aaCG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  1:6869486/1‑76 (MQ=255)

                                                                     aCG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      <  2:1968113/76‑1 (MQ=255)

                                                                       g‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGGGCACCAgccgc    >  1:4650698/1‑76 (MQ=255)

                                                                       g‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc    >  1:6141884/1‑76 (MQ=255)

                                                                       g‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc    >  1:4163224/1‑76 (MQ=255)

                                                                       g‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATAAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc    >  1:501929/1‑76 (MQ=255)

                                                                         ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  2:4400711/1‑76 (MQ=255)

                                                                         ccAACCCCCACCAGATAAAGCATGACGCCTATATAAAATCGTCTTTGTGCCGGTGGCCCTTCCCGCCCCAgccgcc   <  2:4632866/76‑1 (MQ=255)

                                                                          cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:7175213/1‑76 (MQ=255)

                                                                          cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:1600444/1‑76 (MQ=255)

                                                                          cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:4759919/1‑76 (MQ=255)

                                                                          cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:1276065/1‑76 (MQ=255)

                                                                          cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:2775467/1‑76 (MQ=255)

                                                                          cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:3140573/1‑76 (MQ=255)

                                                                          cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:3304747/1‑76 (MQ=255)

AGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCT‑GGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACG‑CCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193705‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`