| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,360,306 | T→A | D656E (GAT→GAA) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,360,306 | 0 | T | A | 99.5% | 862.4 / ‑4.2 | 205 | D656E (GAT→GAA) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (125/79); total (126/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
Only 100 of 208 total aligned reads displayed.
TGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGATGCTG > REL606/2360233‑2360378 | tGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACt < 1:5542912/76‑1 (MQ=255)tGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACt > 2:2681505/1‑76 (MQ=255) ggCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTg < 1:6977990/76‑1 (MQ=255) ggCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTg > 2:4633194/1‑76 (MQ=255) ggCTGACGTGGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTg < 1:3769632/76‑1 (MQ=255) cTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTgca > 1:2374360/1‑74 (MQ=255) gACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAaca > 1:5304051/1‑76 (MQ=255) gACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAaca < 2:5863092/76‑1 (MQ=255) gTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCg > 1:6481847/1‑76 (MQ=255) ttGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCgg > 1:498740/1‑76 (MQ=255) ttGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCgg > 1:146608/1‑76 (MQ=255) ttGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCgg < 2:3445045/76‑1 (MQ=255) ttGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCgg < 1:2656706/76‑1 (MQ=255) gAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAac > 1:1071124/1‑76 (MQ=255) gAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAac < 1:2245098/76‑1 (MQ=255) aaaTCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAaca > 1:4322705/1‑76 (MQ=255) aaaTCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAaca > 1:6689015/1‑76 (MQ=255) aaTCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAacac > 1:126089/1‑76 (MQ=255) aTCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAAcacc < 1:2018409/76‑1 (MQ=255) aTCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAAcacc < 2:5049363/76‑1 (MQ=255) aTCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAAcacc > 1:4631632/1‑76 (MQ=255) ccAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAAcaccac < 2:3353486/76‑1 (MQ=255) cAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACaccacc > 2:1148237/1‑76 (MQ=255) cAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACaccacc < 1:1715752/76‑1 (MQ=255) aaaGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCt > 2:3061685/1‑76 (MQ=255) aaaGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCt < 2:3737912/76‑1 (MQ=255) aaGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTa > 2:2739845/1‑76 (MQ=255) aaGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTa < 2:2240537/76‑1 (MQ=255) aGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTAc > 1:11610/1‑76 (MQ=255) gcCGAACTCTCCGGTTGCAGGTCGCTCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACaaa < 1:7099120/76‑1 (MQ=255) gcCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACaaa > 1:5975871/1‑76 (MQ=255) gcCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACaaa > 1:2274080/1‑76 (MQ=255) ccGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAg < 2:6758457/76‑1 (MQ=255) ccGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAg > 1:1130470/1‑76 (MQ=255) ccGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAg > 1:4713420/1‑76 (MQ=255) aaCTCTCCGGTTGCAGGTCGCGCTACCGTGTTCGTCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCgg > 2:5139390/1‑76 (MQ=255) aaCTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCgg < 2:4969363/76‑1 (MQ=255) aCTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGt > 2:3764309/1‑76 (MQ=255) aCTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGt > 2:5338729/1‑76 (MQ=255) ctctCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTa < 2:691240/76‑1 (MQ=255) ctctCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTa > 1:3686381/1‑76 (MQ=255) tctcCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTAc > 1:5936578/1‑76 (MQ=255) tctcCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTAc > 1:1914393/1‑76 (MQ=255) tctcCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTAc > 1:507654/1‑76 (MQ=255) tctcCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTAc < 2:3259773/76‑1 (MQ=255) tctcCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTAc > 2:6271434/1‑76 (MQ=255) ctcCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACa > 1:6718510/1‑76 (MQ=255) ctcCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACa < 2:7031663/76‑1 (MQ=255) ctcCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACAGGTAACACCACCTACAAAGCGGTACa > 1:5510067/1‑76 (MQ=255) ccGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGc < 1:184610/76‑1 (MQ=255) ccGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGc > 2:2443722/1‑76 (MQ=255) ccGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGc > 2:2121654/1‑76 (MQ=255) cGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCg < 2:5646271/76‑1 (MQ=255) cGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCg > 1:683726/1‑76 (MQ=255) ggTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGt > 1:402664/1‑76 (MQ=255) ggTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGt > 2:777742/1‑76 (MQ=255) ttGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTc < 2:1953915/76‑1 (MQ=255) ttGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTc > 2:3480239/1‑76 (MQ=255) ttGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTc < 2:2843526/76‑1 (MQ=255) ttGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGCACAGCGTTc > 1:3626038/1‑76 (MQ=255) tGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCt > 1:1766065/1‑76 (MQ=255) cAGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGc > 2:3647675/1‑76 (MQ=255) aGGTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGcc > 2:852351/1‑76 (MQ=255) ggTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCg < 2:5605169/76‑1 (MQ=255) ggTCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCg > 2:1571270/1‑76 (MQ=255) tCGCGCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGAc < 1:4474929/76‑1 (MQ=255) cgcgCTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGAcc > 2:2500109/1‑76 (MQ=255) gcgcTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCt < 2:289110/76‑1 (MQ=255) gcgcTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCt < 1:4572814/76‑1 (MQ=255) gcTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGa > 1:1376922/1‑76 (MQ=255) gcTACCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGa < 2:495110/76‑1 (MQ=255) tACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGAtc > 2:657679/1‑76 (MQ=255) aCCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGAtct > 2:5171819/1‑76 (MQ=255) aCCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGAtct > 2:6400600/1‑76 (MQ=255) aCCGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGAtct > 1:2806910/1‑76 (MQ=255) ccGTGTTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGAtctc > 2:738298/1‑76 (MQ=255) gtgtTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTTCCGACCTGATCTCCa < 2:321525/76‑1 (MQ=255) gtgtTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCa > 1:1681528/1‑76 (MQ=255) gtgtTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCa > 1:1547925/1‑76 (MQ=255) gtgtTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCa > 1:3268657/1‑76 (MQ=255) tgtTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCAt > 1:497796/1‑76 (MQ=255) tgtTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCAt > 2:1894767/1‑76 (MQ=255) gtTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATc < 2:4589541/76‑1 (MQ=255) gtTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATc > 1:6900018/1‑76 (MQ=255) gtTCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATc > 1:437463/1‑76 (MQ=255) tCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGTTCTCCATCgg < 2:4876635/76‑1 (MQ=255) tCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCgg > 1:3144938/1‑76 (MQ=255) tCATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCgg > 1:4528465/1‑76 (MQ=255) cATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCggg > 2:4106064/1‑76 (MQ=255) cATCTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCggg > 2:4496388/1‑76 (MQ=255) cTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCg < 2:6845260/76‑1 (MQ=255) cTTCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCg < 1:5772735/76‑1 (MQ=255) tCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGAt > 1:3387129/1‑76 (MQ=255) tCCCGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGAt > 1:5232793/1‑76 (MQ=255) cccGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGAtg > 2:3901152/1‑76 (MQ=255) ccGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGAtgc > 2:1440220/1‑76 (MQ=255) cGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGAtgct > 2:3107274/1‑76 (MQ=255) cGGAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGAtgct < 2:708870/76‑1 (MQ=255) ggAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGAtgctg < 2:6941797/76‑1 (MQ=255) ggAACTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGAtgctg > 1:5195044/1‑76 (MQ=255) | TGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGATGCTG > REL606/2360233‑2360378 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |