| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,875,625 | 1 | . | T | 97.9% | 968.2 / 10.0 | 234 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base T (99/130); total (101/133) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
Only 100 of 274 total aligned reads displayed.
TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875551‑3875695 | tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t < 2:5096609/76‑1 (MQ=255)tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t > 2:7082488/1‑76 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt < 1:6962764/76‑1 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt > 1:1713302/1‑76 (MQ=255) cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGACAAAACTACA‑tttt > 1:5184339/1‑76 (MQ=255) cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt > 1:6590228/1‑76 (MQ=255) cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt < 2:9343892/76‑1 (MQ=255) cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt > 2:9581319/1‑76 (MQ=255) attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttt > 1:10114952/1‑76 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttt‑t > 2:3098123/1‑76 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttt‑t > 2:2837080/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttt‑tt > 2:344119/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttt‑tt > 1:2178812/1‑76 (MQ=255) tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttt‑tttca < 1:1611085/76‑4 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTg < 2:536669/76‑1 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTg > 2:2477207/1‑76 (MQ=255) tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGa > 2:9957239/1‑76 (MQ=255) tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGa < 2:8718079/76‑1 (MQ=255) tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGa > 2:9869238/1‑76 (MQ=255) tACGCGTCCTGACGGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGa < 1:1225040/76‑1 (MQ=255) aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGaa < 1:1352151/76‑1 (MQ=255) cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGaaa < 2:6070937/76‑1 (MQ=255) cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGaaa < 2:3865542/76‑1 (MQ=255) cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATg > 1:3796876/1‑76 (MQ=255) cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATg > 1:7459753/1‑76 (MQ=255) gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATgg < 2:8660511/76‑1 (MQ=255) gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATgg > 1:5938696/1‑76 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGa > 2:9507711/1‑76 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGAc > 2:1097060/1‑76 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGAc > 2:6927207/1‑76 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGAc < 1:4964215/76‑1 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGAc > 1:7777983/1‑76 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAATTACATTTTTT‑TTCATCGTGACATGGACa > 1:5078407/1‑76 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACa > 1:989233/1‑76 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACa < 2:6173064/76‑1 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACag < 1:4254339/76‑1 (MQ=255) gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACaga > 1:5759766/1‑76 (MQ=255) aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACagag < 2:511164/76‑1 (MQ=255) cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAgg > 2:2695303/1‑76 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGa < 2:3961355/76‑1 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGa > 1:1093546/1‑76 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGa < 1:2701765/76‑1 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGa < 1:9075000/76‑1 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGa > 1:7953399/1‑76 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGa > 2:5657034/1‑76 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGAt < 1:7399488/76‑1 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGAt < 1:541285/76‑1 (MQ=255) tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATa < 2:8601974/76‑1 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATaa > 2:8413204/1‑76 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATaa < 2:3375082/76‑1 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATaa < 2:2362417/76‑1 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATaa < 1:10199694/76‑1 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATaa > 1:8636892/1‑76 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATaa > 1:637527/1‑76 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATaaa > 1:9277207/1‑76 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATaaa < 1:5509061/76‑1 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATaaa > 1:1835962/1‑76 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTT‑TTCATCGTGAAATGGACAGAGGATAAAt > 2:4529167/1‑76 (MQ=255) tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAAt < 1:9496545/76‑1 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAAtt > 2:8901696/1‑76 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAAtt > 1:6699736/1‑76 (MQ=255) gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGt > 1:3773997/1‑76 (MQ=255) agaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGtt < 1:4516051/76‑1 (MQ=255) aCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCaa > 2:9012670/1‑76 (MQ=255) aCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCaa < 2:8108943/76‑1 (MQ=255) aCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCaa < 1:5794467/76‑1 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa < 2:4431056/76‑1 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa > 1:7590959/1‑76 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa < 1:4380497/76‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 2:4289370/76‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa > 1:7703893/1‑76 (MQ=255) tttAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg < 1:9855777/76‑1 (MQ=255) tttAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg < 2:2954491/76‑1 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg > 1:6190090/1‑76 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg < 1:4832062/76‑1 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa < 1:2960008/76‑1 (MQ=255) aCCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa < 1:4910185/76‑1 (MQ=255) aCCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa > 2:1874222/1‑76 (MQ=255) aCCGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa < 2:9112260/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 2:4750569/76‑1 (MQ=255) cTTCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc > 1:7242104/1‑76 (MQ=255) ttCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc < 2:7731114/76‑1 (MQ=255) tCACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg < 2:5454209/76‑1 (MQ=255) cACCTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGc > 2:7664974/1‑76 (MQ=255) cTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa < 2:2556933/76‑1 (MQ=255) cTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa < 2:7328501/76‑1 (MQ=255) cTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 1:9602032/1‑76 (MQ=255) cTTGAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa < 2:4638339/76‑1 (MQ=255) cTTGAAAAAACTACA‑TTTTTGTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa < 2:9266434/76‑1 (MQ=255) gAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa < 1:7082488/76‑1 (MQ=255) gAAAAAACTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa > 2:2098723/1‑76 (MQ=255) aaaaaaCTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa < 1:2368575/76‑1 (MQ=255) aaaaCTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg < 1:1175946/76‑1 (MQ=255) aaaaCTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg < 2:6615745/76‑1 (MQ=255) aaaaCTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg < 2:562522/76‑1 (MQ=255) aaaCTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc < 1:3931315/76‑1 (MQ=255) aaaCTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc > 1:2562260/1‑76 (MQ=255) aaaCTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc < 2:3529909/76‑1 (MQ=255) cTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGcc > 1:2742444/1‑76 (MQ=255) cTACATTTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGcc > 1:6084871/1‑76 (MQ=255) | TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTT‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875551‑3875695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |