| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,853,067 | 0 | A | C | 37.7% | 288.9 / 145.5 | 191 | T56P (ACC→CCC) | yeaN | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (107/12); new base C (0/72); total (107/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.09e-40 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 225 total aligned reads displayed.
ACCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTA > REL606/1852992‑1853142 | cGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGGCGACAGCGCAAACCGGCTTATTGaccacc > 2:7146497/1‑76 (MQ=255) cGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGaccacc < 2:9209518/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCt < 1:4571231/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCt < 1:3793736/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCt < 2:1999961/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCt < 1:8355345/76‑1 (MQ=255) gcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGc < 1:4786809/76‑1 (MQ=255) cAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTTACCCCCCTGcc > 2:7711569/1‑76 (MQ=255) cAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGcc > 1:6707693/1‑76 (MQ=255) cAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGcc > 1:6526464/1‑76 (MQ=255) aGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCa < 1:5574536/76‑1 (MQ=255) aGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAAACGGCTTATTTAACACCCCGCCa > 2:9352325/1‑76 (MQ=255) gcgcCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCat < 1:5885666/76‑1 (MQ=255) cggcCGTTACGGGATAGGATTCGTTCCGCTTACTCGCTGAGGACAGCGCAAACCGGCTTATTGACCACCCTGCCat < 2:9305734/74‑1 (MQ=255) ccGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCattat > 1:4491113/1‑76 (MQ=255) ccGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAACGCAAACCGGCTTATTGACCACCCTGCCcttat > 2:2431737/1‑76 (MQ=255) cGTTACTGGATACGTTTCGTCCCTCTTCCCCGCTGACGACAGCTCAACCCGGCTTATTGCCCCCCCTGCCattatt < 2:6476052/76‑1 (MQ=255) cGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCattatt > 1:1914664/1‑76 (MQ=255) gTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCCTTATTg > 1:795140/1‑76 (MQ=255) tACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCCTTATTGGc > 2:4673066/1‑76 (MQ=255) tACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGc > 1:3932899/1‑76 (MQ=255) aCTGGATACGGTTCTTTCCGCTGACTCGCTGATCACAGCGCAAACCGGCTGACTGACCACCCCTCCCTTATCGGcc > 2:9495783/1‑76 (MQ=255) aCTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCAACCTGCCATTATTgggc > 2:145253/1‑74 (MQ=255) aCTCGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGcc > 1:1783979/1‑76 (MQ=255) cTGGATACGTTTCGTCCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCt < 1:2638894/76‑1 (MQ=255) cTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCt > 2:5834778/1‑76 (MQ=255) cTGGATACGATTCGTCCCGCTTACTCGCGGACGACAGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCt < 1:8670519/76‑1 (MQ=255) tGGATACGATTCGTTCCGCTTCCTCGCGGACGACAGCGCAACCCGGTTTATTCCCCCCCCTGCCATTATTGGCCtt < 2:6256750/76‑1 (MQ=255) tGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCtt > 1:8108748/1‑76 (MQ=255) tGGAGACGATTCGTCCCCCTTCCTCGCTGACAACAGCGAAAACGTGTTTATTCCCCCCCCTGCCATTATTGGCCtt < 2:7903794/76‑1 (MQ=255) ggATACGATTCGTTCCTCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCttt < 2:7616336/76‑1 (MQ=255) ggATACGATTCGTTCCGTTTCCTCGCTGACGACAGCCCAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCttt < 2:137010/76‑1 (MQ=255) tACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTgcg > 2:10071844/1‑76 (MQ=255) cgtttCGTCCCGCTTCCTCGCTGACGCCAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCt < 2:3934465/73‑1 (MQ=255) cGATTCGTTCCGCTTACTCGCGGACGACAGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCt < 1:9819013/76‑1 (MQ=255) gATTCTTCCCGCTTCCTCGCGGCCGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTa < 1:7638673/76‑1 (MQ=255) gATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTa > 2:737753/1‑76 (MQ=255) gATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTa > 2:1283457/1‑76 (MQ=255) gATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTa > 2:2355960/1‑76 (MQ=255) aTTCGTTCCGCTTACTCGCGGACGACAGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTaa < 2:2538687/76‑1 (MQ=255) ttCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAAt > 2:5552821/1‑76 (MQ=255) ttCGTCCCGTTTCCTCGCGGACGACAGCGCAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAAt < 2:6871204/76‑1 (MQ=255) ttCGTCCCGCTTCCCCGCGGACGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAAt < 2:4304877/76‑1 (MQ=255) ttCGTCCCCTTTCCTCGCTGACGACACCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAAt < 1:4551774/76‑1 (MQ=255) tgcGTTCCGCTTCCTCGCTGACGACAGCGCAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAAt < 2:5201862/74‑1 (MQ=255) gTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAAtctc > 1:7970796/1‑76 (MQ=255) tCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCAc > 1:7347627/1‑76 (MQ=255) tCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCAc > 1:5241087/1‑76 (MQ=255) cccGTTTCCTCGCTGACGACAGCGCAAACCGGCTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCAc < 2:8356902/75‑1 (MQ=255) ccGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCAcc > 1:1541639/1‑76 (MQ=255) gCTTACTCGCGGACGACAGCGCAAACGGGTTTTTTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCtt < 2:507567/76‑1 (MQ=255) cTTACTCGCTGACGACAGCGAAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCttt < 2:8427486/76‑1 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATTTCCCCTTTg > 2:2220556/1‑76 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg > 2:5634780/1‑76 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg > 1:1370112/1‑76 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg > 1:6837023/1‑76 (MQ=255) ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg > 1:3701229/1‑76 (MQ=255) tgcTCGCGGCCGACAGCACAAACCGGTTTATGGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTgg < 1:4269974/74‑1 (MQ=255) tccTCGCTGACGACAGCGAAAACCGGCTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTgg < 2:4027682/74‑1 (MQ=255) aCTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGc > 2:6364214/1‑76 (MQ=255) aCTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGc > 1:7133142/1‑76 (MQ=255) cTCGCTGACGACAGCCCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCt < 2:4607191/76‑1 (MQ=255) cTCGCGGACGCCAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCt < 2:2671289/76‑1 (MQ=255) cTCGCGGACGACGGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCt < 2:8550349/76‑1 (MQ=255) cggacgacAGCGCAAACCGGCTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGccc < 2:9120252/74‑1 (MQ=255) cTGACAACGGCCCAACCCGGTTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGccc < 2:1494280/76‑1 (MQ=255) tGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGcccc > 2:10060649/1‑76 (MQ=255) gacgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCGTTTGCGCTAATCTCACCTTTGCCTGCCCCg > 1:10195108/1‑76 (MQ=255) gacgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg > 2:4952160/1‑76 (MQ=255) gacgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg > 1:7750677/1‑76 (MQ=255) gacgacAGCCCAACCCGGTTTTTTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg < 2:4822810/76‑1 (MQ=255) acgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCgg > 2:3183077/1‑76 (MQ=255) gacAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTg < 2:7051605/76‑1 (MQ=255) gcAGCACAAGCCGACTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCGCCTTTGGCTGCCCCGGTgg < 2:5913830/75‑1 (MQ=255) acAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTgg > 1:10019029/1‑76 (MQ=255) cggcgaAACCCGGTTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGgc < 1:9017810/74‑1 (MQ=255) cAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGgc > 2:8789721/1‑76 (MQ=255) cgcAACCCGGCTTCTTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGcgcg < 1:4372853/76‑1 (MQ=255) cAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGAc > 2:8959342/1‑76 (MQ=255) acaCCGGTTTTTTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACg < 2:8468751/74‑1 (MQ=255) aaaCCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACg > 2:2880494/1‑76 (MQ=255) aaaCCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACg > 1:2666979/1‑76 (MQ=255) ccGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGttt > 1:3205309/1‑76 (MQ=255) cGGTTTAGTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGtttt < 2:6639000/76‑1 (MQ=255) cGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGtttt > 2:5272107/1‑76 (MQ=255) ggCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTg > 1:2815878/1‑76 (MQ=255) gCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTgg > 2:815017/1‑76 (MQ=255) tttATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGt < 1:2176676/75‑1 (MQ=255) cTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGt < 1:882728/76‑1 (MQ=255) ttATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTa < 2:9708183/76‑1 (MQ=255) ttATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTa > 1:8781693/1‑76 (MQ=255) tATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 1:6272827/1‑76 (MQ=255) tATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 1:6477183/1‑76 (MQ=255) aTTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATg > 1:9292121/1‑76 (MQ=255) tgcacccccCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATgg < 2:3569152/73‑1 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa > 1:1427847/1‑76 (MQ=255) gACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGaa > 1:1653433/1‑76 (MQ=255) gACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGaa > 2:3324778/1‑76 (MQ=255) ccaccCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACg > 1:4465151/1‑76 (MQ=255) accCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTa > 1:5791947/1‑76 (MQ=255) | ACCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTA > REL606/1852992‑1853142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |