| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,344,466 | 0 | T | G | 35.2% | 681.6 / 112.5 | 321 | F515V (TTT→GTT) | acrF | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (45/163); new base G (113/0); total (158/163) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 419 total aligned reads displayed.
CCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACGAT > REL606/3344391‑3344541 | cTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGtt < 2:4077928/76‑1 (MQ=255) tGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGttt < 1:1432200/76‑1 (MQ=255) tGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGttt < 2:1129120/76‑1 (MQ=255) tGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGgtt > 1:1922885/1‑76 (MQ=255) tGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGgtt > 1:6010882/1‑76 (MQ=255) cGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTaa < 2:5220702/76‑1 (MQ=255) gTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAAt < 2:4618652/76‑1 (MQ=255) gTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGGTAAt > 2:8828225/1‑76 (MQ=255) ttATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATa > 2:2461166/1‑76 (MQ=255) ttATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTtata > 2:9340637/1‑76 (MQ=255) ttATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATa > 2:9502991/1‑76 (MQ=255) tATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATac > 1:735066/1‑76 (MQ=255) aTGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATacc < 2:9148416/76‑1 (MQ=255) aTGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTtataac > 1:3499331/1‑74 (MQ=255) tgCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATaccacc < 1:9369069/76‑1 (MQ=255) tgCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATaccaac > 1:3057570/1‑74 (MQ=255) cAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCtt < 2:4460463/76‑1 (MQ=255) cAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCtt < 1:5396439/76‑1 (MQ=255) aaCGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATAACACCTTc > 2:2830128/1‑76 (MQ=255) aaCGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATAACACCCTc > 2:2348757/1‑76 (MQ=255) aCGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCg < 2:2108300/76‑1 (MQ=255) cGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCCTCgc > 1:6263000/1‑75 (MQ=255) gctgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGAt > 2:3234668/1‑76 (MQ=255) ctgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATc < 1:946812/76‑1 (MQ=255) ctgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCCTCCATc > 1:2811552/1‑76 (MQ=255) ctgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATAACACCTTCGATc > 2:9593172/1‑76 (MQ=255) ctgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCCTCGATc > 2:4255264/1‑76 (MQ=255) tgctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATAACACCTTCGATCa > 1:10019756/1‑76 (MQ=255) gctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCAt < 1:3151095/76‑1 (MQ=255) gctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCCTCGATcct > 1:5962927/1‑74 (MQ=255) gctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACAACCCTCGATCAt > 1:5247152/1‑76 (MQ=255) gctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAAAACCACCTTCGATCAt > 1:4845423/1‑76 (MQ=255) ctTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATa < 1:7750772/76‑1 (MQ=255) ttAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAg < 1:6404899/76‑1 (MQ=255) ttAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTGATAACCACTACGAGCATac > 2:8360871/1‑75 (MQ=255) ttAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAg > 1:3948309/1‑76 (MQ=255) tAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTCATAACACCCCTGATCATAGc > 2:1855703/1‑76 (MQ=255) tAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGc < 2:9251572/76‑1 (MQ=255) tAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCCCCTTCGATCATAGc > 2:2981882/1‑76 (MQ=255) aaaCCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCg > 2:8652590/1‑76 (MQ=255) aaaCCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCg > 1:8932958/1‑76 (MQ=255) aaaCCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGGTTATAACACCTTCGATCATAGCg > 1:3718449/1‑76 (MQ=255) aaCCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGt > 2:10188787/1‑76 (MQ=255) cccGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTa < 1:460502/76‑1 (MQ=255) cccGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTa < 1:8178051/76‑1 (MQ=255) ccGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTaa < 1:6172064/76‑1 (MQ=255) gTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAAcc > 2:7263310/1‑76 (MQ=255) tctcTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCATAGGGTTAAccc > 1:51988/1‑75 (MQ=255) ctctGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCAc < 2:6157884/76‑1 (MQ=255) tctGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACt < 2:6955280/76‑1 (MQ=255) tctGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATAACACCCTTGATTATAGCGTTAACCAcc > 2:344909/1‑75 (MQ=255) ctgctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCAACTTCGATCATAGCGGTCACCACTa > 1:2197689/1‑76 (MQ=255) tgctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTac > 1:4989489/1‑76 (MQ=255) tgctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTac < 1:1352274/76‑1 (MQ=255) tgctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTac < 2:3156765/76‑1 (MQ=255) gctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATAACACCTTTGATCATAGCGTTAACCACTaca > 1:6932201/1‑76 (MQ=255) gctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTACTTTCACCCTCGATCCTTGCGCTAACCACTtca > 2:7377523/1‑76 (MQ=255) ctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAACCACTacac > 2:6340493/1‑76 (MQ=255) ctgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAACCACTacac > 2:6051769/1‑76 (MQ=255) tgAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCATAGCGTTAACCACTACAcc > 1:1639331/1‑76 (MQ=255) gAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCa < 1:7076118/76‑1 (MQ=255) gAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCa < 2:7135817/76‑1 (MQ=255) gAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGGTTTATACCACCTTCGATCATAGCGGTAACCCCTACACCa > 2:8540775/1‑76 (MQ=255) aGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCaa < 2:4787928/76‑1 (MQ=255) aGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCaa < 1:7566923/76‑1 (MQ=255) aGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCaa < 1:9760455/76‑1 (MQ=255) aTCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAg < 2:6549917/76‑1 (MQ=255) tCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGc < 2:5961938/76‑1 (MQ=255) tCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGc < 1:8516314/76‑1 (MQ=255) gAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCg < 1:1185067/76‑1 (MQ=255) aaaaTAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCgg < 1:878301/76‑1 (MQ=255) aaaaTAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGcggcgg > 1:2609660/1‑76 (MQ=255) aaaTAAGGGCGGTTTCTTCGGCTGGGTTAATAACACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGc > 2:6759413/1‑76 (MQ=255) aTAAGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaa > 2:1447873/1‑76 (MQ=255) aaGGGCGGTTTCTTCGGCTGGTTTTATACCACCTTCGATCATAGCGTTCACCACCACACCCAAAGCGTCGGCaaaa > 2:3741179/1‑76 (MQ=255) aaGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaaa < 1:2045646/76‑1 (MQ=255) aaGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaaa > 2:4987316/1‑76 (MQ=255) aaGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaaa > 2:788756/1‑76 (MQ=255) aGGGCGGTTTCTTCGGCTGGGTTAATACCACCTTCGCTCATAGCGTTAACCACTACACCAACAGCGTCGGCaaaaa > 2:8081518/1‑75 (MQ=255) ggCGGTTTCTTCGGCTGGGTTTGTAAAACCTTCGGTCATAGCGTTTACCACTTAACAAACAGCGTCAGGaaaaaca > 2:9824655/1‑73 (MQ=255) tcttcGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTc > 1:5555061/1‑76 (MQ=255) cttcGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTcc < 1:2356118/76‑1 (MQ=255) ttcGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCca < 1:7923058/76‑1 (MQ=255) ttcGGCTGGGTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGGTccc > 2:1576561/1‑75 (MQ=255) cGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCcaca < 2:3353092/76‑1 (MQ=255) cGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCcaca < 2:551980/76‑1 (MQ=255) cGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCcaca > 1:4324003/1‑76 (MQ=255) ggCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAg > 2:9678053/1‑76 (MQ=255) ggCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAg > 1:1729389/1‑76 (MQ=255) gCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAgg < 1:9613521/76‑1 (MQ=255) gCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAgg < 2:8021991/76‑1 (MQ=255) gCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAgg < 1:9843665/76‑1 (MQ=255) gCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAgg < 2:8900641/76‑1 (MQ=255) cTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGa > 2:3888131/1‑76 (MQ=255) tgttttAATCCCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGAc < 1:449506/73‑1 (MQ=255) tGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGAc > 1:227078/1‑76 (MQ=255) tGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGAc > 2:5138334/1‑76 (MQ=255) tGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGAc > 2:2526290/1‑76 (MQ=255) ggTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACg > 1:6650996/1‑76 (MQ=255) ggTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACg < 2:10023685/76‑1 (MQ=255) | CCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGCTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGTTCCACAGGACGAT > REL606/3344391‑3344541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |