| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,378,885 | 0 | T | G | 36.6% | 696.5 / 99.8 | 336 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
| Reads supporting (aligned to +/- strand): ref base T (32/181); new base G (123/0); total (155/181) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 416 total aligned reads displayed.
GCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378810‑3378960 | tGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgggt > 2:5821545/1‑76 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGtt < 1:6848660/76‑1 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGtt > 1:6325842/1‑76 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTa < 1:3043936/76‑1 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTa > 2:1933556/1‑76 (MQ=255) cGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGAGATGTTGCGCATCGGCAGGGTGtttct > 1:10198221/1‑73 (MQ=255) gCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCt < 1:4026793/76‑1 (MQ=255) gCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCt > 1:2062334/1‑76 (MQ=255) ccTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTg < 1:8782341/76‑1 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTgg < 1:7280520/76‑1 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTgg < 2:1011249/76‑1 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTgg < 1:5140340/76‑1 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTGCCTGGt > 1:5236793/1‑76 (MQ=255) ttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGtt > 2:2273353/1‑76 (MQ=255) ttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGtt > 2:4793408/1‑76 (MQ=255) tACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGgtt > 2:4386282/1‑76 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTg > 2:4112925/1‑76 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGTTTg > 1:6996967/1‑76 (MQ=255) cTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCCGGTTTGAt > 2:957351/1‑76 (MQ=255) cTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGTTTGAt > 2:9649331/1‑76 (MQ=255) tGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCtggtttggtt > 1:554539/1‑76 (MQ=255) ggTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTTCGCATCGGCAGGGTGTTACCTGGTTTGATTg < 2:7881640/76‑1 (MQ=255) gTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgc < 2:9710972/76‑1 (MQ=255) gTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgc < 2:3663425/76‑1 (MQ=255) ttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcag < 1:2727633/76‑1 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTAGCTAGTTTGAGTgtagc > 2:1077417/1‑76 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTgcagc > 2:4198637/1‑76 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGGTTgcagga > 2:7006554/1‑74 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgtagca > 2:614983/1‑76 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcagca > 2:2045610/1‑76 (MQ=255) aTTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCAt < 2:3702386/76‑1 (MQ=255) aTTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCAt < 2:5611213/76‑1 (MQ=255) tttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATc < 1:5389273/76‑1 (MQ=255) ttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCa < 1:9117167/76‑1 (MQ=255) tCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCaa < 2:10054770/76‑1 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCCAc > 1:651683/1‑76 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTCGCTTGATTTCAGCCTCaca > 2:8357215/1‑74 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGTTTGATTGCAGCATCAAc > 1:4664584/1‑76 (MQ=255) tACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACg < 1:5928827/76‑1 (MQ=255) tACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGGTACCTGGGTTGGTTGCGGCCTCAACg > 1:3788106/1‑76 (MQ=255) tACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCAACg > 1:3393673/1‑76 (MQ=255) aCGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCCTGGGTTGATTGCAGCATCAAAGc > 2:2681110/1‑76 (MQ=255) gTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCa < 2:6951926/76‑1 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGcccg > 2:6567677/1‑74 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAAAGCCAg > 1:6119392/1‑76 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTAACTGGTTTGATTGCAGCATCAACGCCac > 2:5243644/1‑75 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGCGTTACCTGGTTTGTTTGCAGCATAAACGccca > 2:5953738/1‑74 (MQ=255) tATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGa < 1:8471075/76‑1 (MQ=255) tATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGa < 1:1537746/76‑1 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCCTGGTTTGATTGCAGCATCAACCCCAGAc > 1:3605698/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTTCAGCATCAACGCCAGAc > 1:8988491/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTGATTGGAGCCTCAACGCCAGAc > 1:8282530/1‑76 (MQ=255) gAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTg > 2:388087/1‑76 (MQ=255) aaCAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTGATTGCAGCATCAACGCCCGACTGa > 2:8097060/1‑76 (MQ=255) aCAGTAGAACCAACCGGGATGTTGCGCATCGGTAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGaa > 2:4034945/1‑76 (MQ=255) cAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAAt > 1:448074/1‑76 (MQ=255) cAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTTTCTGGTTTGATTGCAGCATCAACGCCAGACTGAAt > 2:6043988/1‑76 (MQ=255) aGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATc < 1:9301432/76‑1 (MQ=255) gTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCt < 1:9846461/76‑1 (MQ=255) gTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCt < 1:3305412/76‑1 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTgg > 1:1986726/1‑76 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTTGTTGCCGCATCAACACCCGACCGAATCttg > 2:496103/1‑74 (MQ=255) aaccaaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTc < 2:9144786/76‑1 (MQ=255) ccaaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGc < 1:6601115/76‑1 (MQ=255) ccaaccGGGATGTTGCGCATCGGCAGGGTGGTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGgcgc > 2:4686344/1‑76 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGcc < 2:9055962/76‑1 (MQ=255) aaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCa < 2:7674170/76‑1 (MQ=255) accGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAg > 2:8631640/1‑76 (MQ=255) ccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGc < 1:3453963/76‑1 (MQ=255) ggATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCttt < 2:452339/76‑1 (MQ=255) ggATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCttt < 2:8717892/76‑1 (MQ=255) gATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAAACTGGGCGCCCGCTTTc > 1:10047169/1‑76 (MQ=255) tGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAg < 1:3929798/76‑1 (MQ=255) tGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAg > 2:4243163/1‑76 (MQ=255) tGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGGCGCCAGCTTTCAg > 2:5345101/1‑76 (MQ=255) gTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAgg < 1:1211886/76‑1 (MQ=255) gTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAgg > 2:1700336/1‑76 (MQ=255) tGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCCTTCAGGcc > 2:6266250/1‑76 (MQ=255) gcgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCt < 1:4192547/76‑1 (MQ=255) cgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCtt < 2:3311166/76‑1 (MQ=255) gcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCttt < 2:1370316/76‑1 (MQ=255) cATCGGCAGGGGGTTACCCGGTTTGATTTCAGCATCAACGCCAGACTGAATCTGGTCGCCCTCCTTCAGGGCTTTa > 2:9829356/1‑76 (MQ=255) cATCGGCAGGGGGGTACCTGGTTTGATTGCAGCATCAACGCCCGACTGAATCTGGTCGCCAGCTTTTAGGCCtttt > 2:8006165/1‑75 (MQ=255) aTCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAg < 1:1300815/76‑1 (MQ=255) aTCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAg < 1:2358794/76‑1 (MQ=255) aTCGGCAGGGGGGTAGCTGGTTTGTTTTTAGCATCAACGCCAGACTGAAACTGGTCGCCCGCTTTCAGGCCttttt > 2:8192504/1‑74 (MQ=255) tCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAgg < 2:49090/76‑1 (MQ=255) tCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAgg < 1:5701844/76‑1 (MQ=255) cGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAggg > 1:9650352/1‑76 (MQ=255) ggCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAgggg < 2:7301485/76‑1 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc < 2:1598189/76‑1 (MQ=255) aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 1:7875458/1‑76 (MQ=255) aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 2:9285366/1‑76 (MQ=255) aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 2:2968605/1‑76 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt < 1:49277/76‑1 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt > 1:481102/1‑76 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt < 1:2915451/76‑1 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt > 1:8579154/1‑76 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt < 2:2500465/76‑1 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCACACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt > 1:5070653/1‑76 (MQ=255) | GCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378810‑3378960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |