| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,320,225 | 0 | T | G | 38.3% | 396.6 / 147.4 | 235 | T40P (ACC→CCC) | melR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (12/133); new base G (90/0); total (102/133) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 314 total aligned reads displayed.
TTTCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGT > REL606/4320150‑4320300 | tttCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCtggt < 1:6058901/76‑1 (MQ=255)tttCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCtggt < 2:1203541/76‑1 (MQ=255) ttCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCcggtg > 1:7390177/1‑76 (MQ=255) tCATTGTTGATCAGGTATTCCACATCGCCATCTAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCtggggg > 1:4787121/1‑76 (MQ=255) tCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCtggtgg < 2:9351825/76‑1 (MQ=255) tCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCGGACCATGCCAGTGGCtggtgg < 2:2638201/76‑1 (MQ=255) cATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTggg < 2:6960540/76‑1 (MQ=255) aTTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTggggggg > 1:9368490/1‑75 (MQ=255) aTTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGc > 1:5102079/1‑76 (MQ=255) ttgttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTgggggggg > 1:8182861/1‑74 (MQ=255) tgttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTggggggggg > 1:55849/1‑73 (MQ=255) tgttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTgggggggcg > 1:3409515/1‑73 (MQ=255) tgttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGcgg > 2:6317785/1‑74 (MQ=255) gttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATg < 2:5831626/76‑1 (MQ=255) gttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATg < 1:395079/76‑1 (MQ=255) gttgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATg > 1:10014264/1‑76 (MQ=255) tgATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGat < 2:5930463/76‑1 (MQ=255) gATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGata < 1:2433448/76‑1 (MQ=255) gATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGCTGata > 1:2349546/1‑76 (MQ=255) aTCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGatat < 2:7190638/76‑1 (MQ=255) tCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATg < 2:3436331/76‑1 (MQ=255) aGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATgtg > 2:4380235/1‑74 (MQ=255) aGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCg < 2:3759392/76‑1 (MQ=255) aGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCg < 1:3724338/76‑1 (MQ=255) gTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGt < 1:1920751/76‑1 (MQ=255) gTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGCCGGATATggggg > 1:1703794/1‑75 (MQ=255) gTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGCACGATATGCggg > 2:6580809/1‑75 (MQ=255) tATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGGTATgggggg > 1:8675495/1‑74 (MQ=255) aTTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTgc < 1:692525/76‑1 (MQ=255) aTTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGGGATATGCggggg > 1:3983866/1‑73 (MQ=255) aTTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATATggggggg > 2:5746095/1‑73 (MQ=255) ttCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATATGCGGTggg > 2:1359071/1‑74 (MQ=255) ccACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGcgcg < 2:1506537/76‑1 (MQ=255) ccACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGcgcg < 2:8396539/76‑1 (MQ=255) ccACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTTATATGCggcgggcg > 1:4962253/1‑76 (MQ=255) cacaTCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGTTGCGCGa < 2:1698288/76‑1 (MQ=255) cacaTCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCACGATATGTGGGGGGCGa > 2:3820944/1‑76 (MQ=255) tCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACt < 2:7936517/76‑1 (MQ=255) tCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACt < 2:6209861/76‑1 (MQ=255) cGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGGTATGCGGGGGGCGGAACTc > 2:9283236/1‑76 (MQ=255) gCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCa < 2:1130201/76‑1 (MQ=255) gCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCa < 1:2786753/76‑1 (MQ=255) gCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCa < 1:8390420/76‑1 (MQ=255) gCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATATGCGGGGCGCGGAACTCa > 1:597888/1‑76 (MQ=255) cATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATATTGGGTGGGCGAAACTCAAt > 2:9090360/1‑76 (MQ=255) cATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATATGCGGTGCGCGCAACTCAAt > 1:4427843/1‑76 (MQ=255) gAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGGTATGCGGTGCGGGGCACTCCATTTcc > 1:1077287/1‑76 (MQ=255) gAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGATATGCGGGGCGCGAAAACCAATCTcc > 2:8304289/1‑76 (MQ=255) gAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATATGCGGGGCGCGAAACCCAAACTcc > 2:2392770/1‑76 (MQ=255) aaaGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATATGCGGTGCGCGAAACTCAATCTccc > 2:2986422/1‑75 (MQ=255) aaGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGCAGATATGTGGGGGGCTAAATCCAATCACCAt > 2:414669/1‑76 (MQ=255) aGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATATGCGGGGCGCGAAAATCAATCTCCCTc > 1:8494579/1‑76 (MQ=255) cacaTTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGGATGAGATGCGGTGCGCGAAAATCAAATTCCATcccc > 1:7539712/1‑74 (MQ=255) cacaTTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGGTATGCGGTGGGCGGAACTCAAACTCCCTCCGc > 2:5282951/1‑76 (MQ=255) cacaTTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGCATGATAAGCGGGGCGCGAAACTCCATCTCCATCCGc > 2:7630020/1‑76 (MQ=255) acaTTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCt < 2:2128623/76‑1 (MQ=255) caTTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTg < 2:6712313/76‑1 (MQ=255) aTTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTgg < 1:1553047/76‑1 (MQ=255) aTTCACTTCGACCTGACCATGCCAGTGGCTGGGGGGGGTGATATGCGGGGCGCGAAACTCAAACTCCATCCCCTgg > 1:1981347/1‑76 (MQ=255) ttCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGt < 2:4961184/76‑1 (MQ=255) ttCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGt < 1:3008639/76‑1 (MQ=255) ttCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGt < 2:3241716/76‑1 (MQ=255) cACTTCGACCTGACCATGCCAGTGGCTGGGGGGGATGATATGCGGGGCGCGAAACTCAATCTCCCTCCCCCGGTAt > 1:4211266/1‑76 (MQ=255) aCTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTAtt < 2:1986180/76‑1 (MQ=255) cTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTc < 2:9653658/76‑1 (MQ=255) cTTCGACCTGACCATGCCAGTGGCTGGGGGGCATGATATGCGGTGCGCGAAAATCAATCTCCATCCGCTGGGATTc > 1:10082017/1‑76 (MQ=255) tCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCg < 2:4612468/76‑1 (MQ=255) gACCTGACCATGCCAGTGGCTGGGGGGGATGATATGCGGGGCGCGAAACTCAATCTCCCTCCGCCGGGATTCCgca > 1:6325420/1‑74 (MQ=255) aCCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAAt < 2:5654505/76‑1 (MQ=255) ccTGACCATGCCAGTGGCTGGGGGGCATGATATGCGGGGCGCGAAACTCAATCTCCATCCGCTGGGATTTCGAATa > 1:1336085/1‑76 (MQ=255) cTGACCATGCCAGTGGCTGGGGGGGATGATATTCGGTGCGCGGAACTCAATCTCCATCCGCCGGGATTTCCAATac > 1:5023257/1‑76 (MQ=255) cTGACCATGCCAGTGGCTGGGGGGGATGATATGCGGGGGGCGGAACCCCATCTCCCTCCGCTGGGATTTCCAATaa > 2:8812363/1‑75 (MQ=255) cTGACCATGCCAGTGGCTGGGGGGGATGATATGCGGGGCGCGAAAATCAAACTCCATCCCCTGGGTTTTCGCATac > 2:8637590/1‑76 (MQ=255) tGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATaca < 2:3665475/76‑1 (MQ=255) tGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATaca < 1:2929058/76‑1 (MQ=255) tGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATaca < 2:10172824/76‑1 (MQ=255) tGACCATGCCAGTGGCTGGGGGGGCCGGTATGCGGGGCGCGAAACACCATCTCCCTTCGCTGGTTTTCCCAATaca > 2:3634118/1‑76 (MQ=255) aCCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagc < 2:2858072/76‑1 (MQ=255) aCCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagc < 1:6635388/76‑1 (MQ=255) ccATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagcg < 1:2443816/76‑1 (MQ=255) ccATGCCAGTGGCTGGGGGGGATGATATGCGGGGCGCGAAACTCAAACTCCATCCGCCGGGATTCCCAATacaacg > 2:9426934/1‑76 (MQ=255) aTGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagcgac < 1:8870416/76‑1 (MQ=255) tGCCAGTGTCTGGTGGGCATGATAAGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagcgaca < 1:5076649/76‑1 (MQ=255) ccAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATacagcgacagc < 1:4586208/76‑1 (MQ=255) aGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCgg < 1:7932580/76‑1 (MQ=255) gTGGCTGGGGGGGATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTTCGAATACACAGACAGCggg > 2:5173556/1‑76 (MQ=255) tGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCTACAGCGGGc > 2:7320288/1‑76 (MQ=255) tGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGc < 1:7707556/76‑1 (MQ=255) tGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGc > 2:2507484/1‑76 (MQ=255) ggCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCt < 1:9038717/76‑1 (MQ=255) gCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTg < 1:7069267/76‑1 (MQ=255) gCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTg < 1:7856876/76‑1 (MQ=255) gCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTg > 1:7798131/1‑76 (MQ=255) cTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGc > 2:487975/1‑76 (MQ=255) cTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGc > 2:205668/1‑76 (MQ=255) ggtggGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCgg < 1:5811681/76‑1 (MQ=255) gtggGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCggg > 2:2312566/1‑76 (MQ=255) gtggGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCggg > 2:5555909/1‑76 (MQ=255) tggGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGt > 1:5881081/1‑76 (MQ=255) tggGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGt > 1:6548761/1‑76 (MQ=255) | TTTCATTGTTGATCAGGTATTCCACATCGCCATCGAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGT > REL606/4320150‑4320300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |