| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,392,556 | 0 | C | T | 39.6% | 252.5 / 151.4 | 225 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (97/39); new base T (0/89); total (97/128) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.84e-32 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 335 total aligned reads displayed.
AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 | aGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTc < 2:5270229/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTa > 1:1475380/1‑76 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 2:7200050/76‑1 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg < 2:7110344/76‑1 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg < 2:692805/76‑1 (MQ=255) aCTTATACCTGTCTGATGGCGCGCTCTGCAAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGc < 2:2886864/76‑1 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc > 2:7513625/1‑76 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc > 2:6533503/1‑76 (MQ=255) cTTATACCGGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 1:1628609/76‑1 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa < 2:2450484/76‑1 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa > 2:779895/1‑76 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa > 2:3592443/1‑76 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCCATTTTCTAGCCa > 2:8280456/1‑76 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGGGATTTTCTAGcccg > 1:2691944/1‑74 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAg > 1:3327650/1‑76 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg > 1:9770369/1‑76 (MQ=255) atcccTGTCTGTGGGCGCTCTCTGCGAAGCGGTAACGCGTGAGGGTGCTGGCGGGGATAGCTTTTTTTTAGCCAGt < 2:9154338/73‑1 (MQ=255) atcccTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGGGTGAGGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGt < 2:4556400/73‑1 (MQ=255) ccTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGAATTTCTAGCCAGTAtt > 2:1319459/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGGCAGGATTg > 2:385765/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTg > 1:3213645/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTg > 2:1799227/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTg > 2:1700077/1‑76 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGGGGTTTTCTTGCCAGTATTTGt > 1:1069999/1‑76 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGt > 2:5193676/1‑76 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGt > 1:2133268/1‑76 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGACAGGATTggg > 2:4448897/1‑75 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGGca > 1:2928552/1‑76 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTcag > 1:4781678/1‑76 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTcag > 2:928425/1‑76 (MQ=255) tGAGGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTcag < 2:9040092/76‑1 (MQ=255) gATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTcagc < 2:5833589/76‑1 (MQ=255) gcgcgcGGCGAAGCGTTAAGGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAg < 2:2645984/76‑1 (MQ=255) gcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAg > 2:9401818/1‑76 (MQ=255) gcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAg < 1:8117514/76‑1 (MQ=255) gcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGGTTTTCTAGCCAGTATTGGTTAGCCGTTAGGa > 1:2838454/1‑76 (MQ=255) gcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGGAGTTAGGa > 2:1564984/1‑76 (MQ=255) tctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAAc > 2:8715464/1‑76 (MQ=255) tctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAAc < 1:210796/76‑1 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACg > 1:5300281/1‑76 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACg > 1:6414782/1‑76 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACg < 1:6245829/76‑1 (MQ=255) tGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACgg > 1:71796/1‑76 (MQ=255) tGCGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACgg < 1:9046576/76‑1 (MQ=255) tGCAAAGCGGTAACGGGTGAAGGTGCTGGGTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACgg < 1:3233197/76‑1 (MQ=255) gCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGc > 2:2375759/1‑76 (MQ=255) aggCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGa < 1:830369/74‑1 (MQ=255) aaGCGGTAACGGGGGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGa < 2:4035032/76‑1 (MQ=255) aaGCGGAAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGa < 2:6783103/76‑1 (MQ=255) ggTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATcc < 1:5123208/76‑1 (MQ=255) ggTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATcc > 1:1282843/1‑76 (MQ=255) gatAACGGGTGAGGGGGTGGGCTGGGATAGCAATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATcc < 1:7848200/74‑1 (MQ=255) gTAACGCGTGAAGGTGCTGGCTGGGATAGCTATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATccc < 1:7699050/76‑1 (MQ=255) gTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATacc > 1:3121899/1‑76 (MQ=255) tAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAAACCCt > 2:5436354/1‑76 (MQ=255) aaCGGGGGAAGGTGCTGGGTGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCtt < 2:3953662/76‑1 (MQ=255) aaCGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAAACCCtt > 2:1456105/1‑76 (MQ=255) aCGCGTGAAGGTGCTGGCGGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTg < 2:9658719/76‑1 (MQ=255) cgcgTGAAGGTGCTGGCTGGGATAGCTATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGa < 2:9805474/76‑1 (MQ=255) cgcgGGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGa < 2:123475/76‑1 (MQ=255) cgcgGGAGGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGa < 1:8563332/76‑1 (MQ=255) gggTGAGGGTGCTGGTTGGGATAGTGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa < 1:692707/74‑1 (MQ=255) gcgTGAAGGGGCTGGTTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa < 2:4324371/76‑1 (MQ=255) cgTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAAc > 1:4852810/1‑76 (MQ=255) gTGAAGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACt < 2:643833/76‑1 (MQ=255) tGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTg > 1:7443350/1‑76 (MQ=255) tGAAGGGGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTg < 1:6584363/76‑1 (MQ=255) gAAGGTGCGGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgg < 1:3794382/76‑1 (MQ=255) aaGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTggg < 1:2405631/76‑1 (MQ=255) aaGGGGCTGGGTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTggg < 1:3435144/76‑1 (MQ=255) aGGTGCTGGCTGGGATAGCGATTTTCTAGCCCGTATTGGTCCGCAGTTAGGAAAGGCTGGATCCCTTGCACTgggg > 2:8545735/1‑76 (MQ=255) gTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCCTGAACTGGggcg > 1:8687428/1‑76 (MQ=255) gTGCGGTCTGGGATGGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcg < 1:1719362/76‑1 (MQ=255) tGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCCTGAACTGgggggg > 2:1626606/1‑76 (MQ=255) ctggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCt > 1:7915518/1‑76 (MQ=255) tggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATTCCTTGAACTGGggcgggcg > 2:10005025/1‑73 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg > 2:2165432/1‑76 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCCgg > 2:8804696/1‑76 (MQ=255) ggcgggGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 1:2495532/76‑1 (MQ=255) gtggggATGGTGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGc < 1:5982507/73‑1 (MQ=255) gctggGATGGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGc < 1:1930764/76‑1 (MQ=255) ctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgggg > 2:202363/1‑74 (MQ=255) ctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgggg > 1:5478593/1‑74 (MQ=255) ctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgggg > 1:7032620/1‑74 (MQ=255) ctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCg > 1:10062413/1‑76 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTggggg > 1:5725704/1‑73 (MQ=255) ggagATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGa < 2:6250876/73‑1 (MQ=255) gggATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGaa > 1:2251334/1‑76 (MQ=255) ggATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGGAt > 2:554347/1‑76 (MQ=255) gATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtg < 2:4927423/76‑1 (MQ=255) gATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGTGAAtg > 1:4960965/1‑76 (MQ=255) gtAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtgt < 1:6832836/75‑1 (MQ=255) aTAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtgt < 2:3512618/76‑1 (MQ=255) aGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGa > 1:9191872/1‑76 (MQ=255) gttttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAAt < 2:3496133/73‑1 (MQ=255) cttttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAAt < 1:3597664/73‑1 (MQ=255) cttttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAAt < 2:3327650/73‑1 (MQ=255) cgtttttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAAt < 1:4557591/73‑1 (MQ=255) tttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATcc < 1:8205772/75‑1 (MQ=255) tttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATcc < 1:4199345/75‑1 (MQ=255) | AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |