| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,650,140 | A→G | R69G (AGA→GGA) | yiaG → | predicted transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,650,140 | 0 | A | G | 97.6% | 1245.0 / 9.6 | 289 | R69G (AGA→GGA) | yiaG | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (135/147); total (138/151) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 290 total aligned reads displayed.
GACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGC > REL606/3650067‑3650212 | gACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGa < 2:7326324/76‑1 (MQ=255)gACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGa > 2:1106931/1‑76 (MQ=255) aCAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGAc < 1:4055973/76‑1 (MQ=255) aCAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGAc < 1:503799/76‑1 (MQ=255) cAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGAcg < 2:4575380/76‑1 (MQ=255) cAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGAcg < 1:6455175/76‑1 (MQ=255) aGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGAcgc > 1:4664805/1‑76 (MQ=255) aGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGAcgc > 2:5479915/1‑76 (MQ=255) gATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGt < 1:8863595/76‑1 (MQ=255) aTTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTg < 1:1421472/76‑1 (MQ=255) aTTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTg > 2:5588844/1‑76 (MQ=255) ttAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGa < 1:99464/76‑1 (MQ=255) ttAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGa < 2:7614681/76‑1 (MQ=255) ttAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGa < 1:2093424/76‑1 (MQ=255) tAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGaa < 1:3401441/76‑1 (MQ=255) aaaaaTCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAg < 1:5454831/76‑1 (MQ=255) aaaaaTCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAg > 1:9465872/1‑76 (MQ=255) aaTCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGCAAAGGAATGGGAATCCGGACGCGTGAAGCCt > 1:8264915/1‑76 (MQ=255) cGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCa < 1:4368611/76‑1 (MQ=255) cGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCa < 1:2489646/76‑1 (MQ=255) cGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCa > 1:476619/1‑76 (MQ=255) cGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCa > 1:1986065/1‑76 (MQ=255) cGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCa < 1:1232935/76‑1 (MQ=255) gatgatTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCaa < 1:1561379/76‑1 (MQ=255) atgatTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAg < 2:1745440/76‑1 (MQ=255) atgatTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAg < 1:1808024/76‑1 (MQ=255) tgatTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGt < 1:4825819/76‑1 (MQ=255) gatTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTg > 1:5906089/1‑76 (MQ=255) tttCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGcc > 2:1836802/1‑76 (MQ=255) tttCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGcc < 2:3347169/76‑1 (MQ=255) tttCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGcc < 2:6313524/76‑1 (MQ=255) ttCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCg < 1:6208582/76‑1 (MQ=255) cGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGaa < 2:8950826/76‑1 (MQ=255) cGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGaa < 2:5787373/76‑1 (MQ=255) cccGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACt < 1:5789181/76‑1 (MQ=255) cccGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACt > 2:8397885/1‑76 (MQ=255) ccGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTa < 1:8115906/76‑1 (MQ=255) cGGGTTTTGGGCGTGTCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:1224963/1‑76 (MQ=255) cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:6981407/1‑76 (MQ=255) cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:7423793/1‑76 (MQ=255) cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa < 2:3438030/76‑1 (MQ=255) cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:4391789/1‑76 (MQ=255) cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:4134816/1‑76 (MQ=255) cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:9669366/1‑76 (MQ=255) cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa < 1:7317028/76‑1 (MQ=255) gggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaa > 1:1029699/1‑76 (MQ=255) gggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaa > 2:2920513/1‑76 (MQ=255) gggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaa > 2:1537141/1‑76 (MQ=255) gggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaa < 1:5289710/76‑1 (MQ=255) gggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaa < 1:9669366/76‑1 (MQ=255) ggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaaa < 1:9615996/76‑1 (MQ=255) tttGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTg > 1:8415832/1‑76 (MQ=255) tGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGAt > 1:1013082/1‑76 (MQ=255) gggCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATg > 2:6065062/1‑76 (MQ=255) ggCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGc < 1:4447619/76‑1 (MQ=255) gTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGtt < 1:2397530/76‑1 (MQ=255) tATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGttt < 2:449817/76‑1 (MQ=255) tATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGttt > 2:9266523/1‑76 (MQ=255) aTCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTg < 2:8818885/76‑1 (MQ=255) tCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGa < 1:2551681/76‑1 (MQ=255) tCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGa < 1:7501882/76‑1 (MQ=255) tCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCa < 1:7983645/76‑1 (MQ=255) tCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCa < 2:8251825/76‑1 (MQ=255) tCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCa < 2:8626826/76‑1 (MQ=255) cGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCaa > 2:6178292/1‑76 (MQ=255) gCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAg < 1:3197321/76‑1 (MQ=255) gCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAg < 1:6998412/76‑1 (MQ=255) ccATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGc > 1:6063805/1‑76 (MQ=255) ccATGGTAAAGGAATGGGAATCCGGACGCGTAAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGc > 2:7879692/1‑76 (MQ=255) cATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGcc > 1:5619679/1‑76 (MQ=255) cATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGcc > 2:9853064/1‑76 (MQ=255) aTGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCa < 2:1296076/76‑1 (MQ=255) tGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCaa > 1:5129242/1‑76 (MQ=255) tGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCaa < 2:3943047/76‑1 (MQ=255) tGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCaa > 1:3009784/1‑76 (MQ=255) tGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCaa < 2:6622075/76‑1 (MQ=255) tGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCaa > 2:1170467/1‑76 (MQ=255) ggTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAc > 1:667551/1‑76 (MQ=255) ggTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAc < 1:1727032/76‑1 (MQ=255) gTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAcc > 2:8910604/1‑76 (MQ=255) tAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAccc < 2:2300839/76‑1 (MQ=255) tAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAccc < 2:2850963/76‑1 (MQ=255) tAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAccc > 1:8650832/1‑76 (MQ=255) aaaGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCg < 2:5059147/76‑1 (MQ=255) aaaGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCg > 2:3589597/1‑76 (MQ=255) aaGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCgg < 2:6496736/76‑1 (MQ=255) gAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCAt < 2:4928674/76‑1 (MQ=255) aaTGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCAtt < 1:10039599/76‑1 (MQ=255) aaTGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCAtt < 2:5268137/76‑1 (MQ=255) aTGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATta < 1:3766159/76‑1 (MQ=255) aTGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATta > 1:10015869/1‑76 (MQ=255) gggAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaag < 1:790075/76‑1 (MQ=255) gggAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaag > 2:2468115/1‑76 (MQ=255) ggAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaagt < 1:2148869/76‑1 (MQ=255) gAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaagta > 1:9570973/1‑76 (MQ=255) gAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaagta < 2:3234031/76‑1 (MQ=255) aaTCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaagtaa < 2:7149070/76‑1 (MQ=255) aTCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaagtaag > 2:2292151/1‑76 (MQ=255) tCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGc < 1:9148005/76‑1 (MQ=255) tCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGc < 1:2730900/76‑1 (MQ=255) | GACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGC > REL606/3650067‑3650212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |