| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,168,348 | G→T | G1130C (GGT→TGT) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,168,348 | 0 | G | T | 99.3% | 1221.9 / 1.0 | 296 | G1130C (GGT→TGT) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base T (149/145); total (151/145) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01 | |||||||||||
Only 100 of 311 total aligned reads displayed.
GATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGG > REL606/4168275‑4168420 | gATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGt > 1:3159039/1‑76 (MQ=255) aTTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGTAGGAATCCGGCTGTa > 1:6482335/1‑76 (MQ=255) aTTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTa > 2:6968191/1‑76 (MQ=255) tGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTAcc > 1:5062399/1‑76 (MQ=255) gTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCa < 1:1263587/76‑1 (MQ=255) tCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAg > 2:119329/1‑76 (MQ=255) tCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAg < 2:7686417/76‑1 (MQ=255) tCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAg < 2:1724105/76‑1 (MQ=255) tCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAg < 2:252831/76‑1 (MQ=255) cAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAgg < 1:3781096/76‑1 (MQ=255) aGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGa < 2:3350127/76‑1 (MQ=255) cTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACa > 2:5497629/1‑76 (MQ=255) tGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACAt > 2:2351504/1‑76 (MQ=255) ggAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATc > 1:823075/1‑76 (MQ=255) gAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCa < 2:1085022/76‑1 (MQ=255) gAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCa > 2:9066250/1‑76 (MQ=255) gaGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCAc < 1:3916662/75‑1 (MQ=255) aaGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCAc > 2:7401634/1‑76 (MQ=255) aGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCAcc > 2:2688860/1‑76 (MQ=255) gATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCg > 2:3912882/1‑76 (MQ=255) gATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCg > 2:5065119/1‑76 (MQ=255) aTGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCgg < 1:1652277/76‑1 (MQ=255) tGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCggt > 2:1418446/1‑76 (MQ=255) ggCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCggtg > 1:2954315/1‑76 (MQ=255) cGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCggtggt > 1:9090696/1‑76 (MQ=255) cGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCggtggt > 1:9706054/1‑76 (MQ=255) gTACAGATCAGCTCTGGTGACTCCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTc < 2:5897743/76‑1 (MQ=39) gTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTc > 2:7191403/1‑76 (MQ=255) tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCt > 1:8587102/1‑76 (MQ=255) tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCt < 1:7997424/76‑1 (MQ=255) tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCt < 2:5699794/76‑1 (MQ=255) tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCt > 2:6305878/1‑76 (MQ=255) aCAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTg > 2:2225631/1‑76 (MQ=255) aCAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTg < 2:1903019/76‑1 (MQ=255) aCAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTg < 2:2892711/76‑1 (MQ=255) aCAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTg > 2:3264411/1‑76 (MQ=255) cAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGc > 1:8767565/1‑76 (MQ=255) aGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGcc < 1:1410677/76‑1 (MQ=255) gATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcg > 1:4462001/1‑76 (MQ=255) aTCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcgc > 2:4672571/1‑76 (MQ=255) tCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcgcg > 2:2821744/1‑76 (MQ=255) cAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcgcgc > 2:4599724/1‑76 (MQ=255) cAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcgcgc > 1:1076188/1‑76 (MQ=255) aGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcgcgcg > 1:901569/1‑76 (MQ=255) ctctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTTTGCGGCGCGtt > 1:7825699/1‑76 (MQ=255) ctctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGtt < 1:4801136/76‑1 (MQ=255) ctctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGtt < 1:9919616/76‑1 (MQ=255) ctctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGtt < 2:4928177/76‑1 (MQ=255) ctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGc > 1:9147423/1‑76 (MQ=255) ctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGc > 1:8664119/1‑76 (MQ=255) ctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGc > 1:6075749/1‑76 (MQ=255) ggTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCgg < 2:4266817/76‑1 (MQ=255) ggTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCgg > 1:4912198/1‑76 (MQ=255) ggTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCgg < 1:2431840/76‑1 (MQ=255) gACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCGAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGAcc < 1:2777999/76‑1 (MQ=255) acacCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCt < 1:4544966/76‑1 (MQ=255) acacCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCt < 2:10179893/76‑1 (MQ=255) cacCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTg < 2:6137336/76‑1 (MQ=255) cccTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGtt < 1:1973663/76‑1 (MQ=255) ccTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTc > 1:6571931/1‑76 (MQ=255) ccTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTc < 1:10055581/76‑1 (MQ=255) ccTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTc < 2:2067284/76‑1 (MQ=255) cTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCg < 1:5160240/76‑1 (MQ=255) tGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGa < 1:6340200/76‑1 (MQ=255) tGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGa > 2:3661203/1‑76 (MQ=255) tGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGa < 1:7285816/76‑1 (MQ=255) tGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGa > 2:9440005/1‑76 (MQ=255) tGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGa < 1:9856774/76‑1 (MQ=255) ggCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGaa > 2:9169717/1‑76 (MQ=255) gcgcGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAg < 2:743907/76‑1 (MQ=255) cgcgTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGc > 2:9592052/1‑76 (MQ=255) cgcgTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGc < 1:9556958/76‑1 (MQ=255) cgTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAc > 2:4843185/1‑76 (MQ=255) gTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcg < 2:6755029/76‑1 (MQ=255) tATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgt < 1:764006/76‑1 (MQ=255) tATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgt > 2:3190011/1‑76 (MQ=255) tATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACATGTTCGAAGCAcgt > 1:6197251/1‑76 (MQ=255) aTTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtc < 2:8879711/76‑1 (MQ=255) aTTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtc < 2:821741/76‑1 (MQ=255) aTTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtc < 2:4107686/76‑1 (MQ=255) aTTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtc > 1:5972731/1‑76 (MQ=255) ttCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtcg < 2:4408222/76‑1 (MQ=255) ttCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtcg < 2:9743770/76‑1 (MQ=255) ccGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACgtcgtc > 2:3941260/1‑76 (MQ=255) ccGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACgtcgtc < 2:8154220/76‑1 (MQ=255) ccGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACgtcgtc < 1:3370585/76‑1 (MQ=255) ccGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACgtcgtc < 2:8966934/76‑1 (MQ=255) gCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCg < 1:2359410/76‑1 (MQ=255) gCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCg < 1:8323838/76‑1 (MQ=255) gCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCg < 1:3588046/76‑1 (MQ=255) cAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGa > 2:8796904/1‑76 (MQ=255) ggAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGaaa < 2:9855174/76‑1 (MQ=255) ggAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGaaa > 1:4000162/1‑76 (MQ=255) ggAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGaaa > 1:6831986/1‑76 (MQ=255) gAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAag < 2:5230599/76‑1 (MQ=255) aTCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAagag < 1:5633421/76‑1 (MQ=255) tCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGc > 2:8528302/1‑76 (MQ=255) tCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGc > 1:2137267/1‑76 (MQ=255) ccGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGcc > 1:1750699/1‑76 (MQ=255) ggctgTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCgg > 2:5809925/1‑76 (MQ=255) | GATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGG > REL606/4168275‑4168420 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |