| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 99.6% | 859.6 / NA | 222 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (71/149); total (71/151) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 253 total aligned reads displayed.
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 | gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATcc < 2:5183448/76‑1 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGCGGC‑GGGAAA‑CAGCCCCCATAAACACAAAACACCCATcccc > 1:635345/1‑76 (MQ=255) gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCcca < 2:9832139/76‑1 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCccac < 1:8929624/76‑1 (MQ=255) tGCCTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCccacc < 2:1987159/76‑1 (MQ=255) tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCccacc < 1:918697/76‑1 (MQ=255) tGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAAA‑AACCCCCAAAAAAACAAAAACCCCAACcccccc > 2:4495115/1‑74 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGAAAT‑CAGCCCCCATAACAGCAAAACGCCAATCCccacca > 1:7176298/1‑76 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg < 1:6183783/76‑1 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg < 1:7450448/76‑1 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg < 2:3723144/76‑1 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTT‑GGGAAA‑CCGCCCCAAAAAAACCAAAACAACAAAACCCCCCAg > 2:7577325/1‑76 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAAA‑CAGCCCCCATAAAAGCAAAACGCCAAACCcccccac > 1:1623268/1‑75 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAAA‑CAGCCCCCATAAAAGCAAAACACCCAACCccacccg > 1:2619200/1‑74 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGGAAA‑CAGCCCCAATAACAGCAAAAAGCCAAAACcccccac > 1:5481302/1‑75 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAAACCccacccg > 1:10066241/1‑74 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCCGTT‑GGGAATCCCACCCCAAAAAAGGCAAAAC‑TCAACACCCACCAg > 2:1375687/1‑76 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGaa < 2:5484709/76‑2 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 2:7290025/76‑1 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 1:9598972/76‑1 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 2:8245437/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa < 2:9079038/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAACCCCCACCAGATa < 2:7092542/76‑1 (MQ=255) cGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa < 1:3255046/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 2:3045306/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 1:9333643/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 1:10128900/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGAAAT‑CAGCCCCCATAACAGCAAAAAGCCAATCCCCACCAGATaaa > 1:4501529/1‑76 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTTTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 2:1677146/76‑1 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 1:4775729/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc < 1:4255587/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc < 1:5170177/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc < 1:6579588/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGGCTGGCTGTC‑GGAAAA‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc > 1:2385120/1‑76 (MQ=255) gTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa < 2:1216490/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 2:2193772/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 1:8629433/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 1:9056855/76‑1 (MQ=255) ccAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 1:2089237/76‑1 (MQ=255) ccAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 2:7451176/76‑1 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 2:1054919/76‑1 (MQ=255) ggCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg < 2:1521590/76‑1 (MQ=255) cgcgGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGcc < 2:637607/76‑1 (MQ=255) gcgGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 2:3010142/76‑1 (MQ=255) gcgGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 1:497854/76‑1 (MQ=255) cgGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:8722989/76‑1 (MQ=255) ggCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt < 1:6013949/76‑1 (MQ=255) gCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa > 2:4843937/1‑76 (MQ=255) cTGGTCTGGCTGTC‑GGTAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACTCCTGTAt < 2:3928914/76‑1 (MQ=255) cTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 1:3386275/76‑1 (MQ=255) ggTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 2:6527934/1‑76 (MQ=255) ggTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 2:5313516/1‑76 (MQ=255) ggTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 2:4450270/76‑1 (MQ=255) ggTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 2:8603925/1‑76 (MQ=255) gTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa > 1:3888420/1‑76 (MQ=255) gTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:8539594/76‑1 (MQ=255) tCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 1:4629467/76‑1 (MQ=255) tCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 2:10148075/76‑1 (MQ=255) cTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc > 1:8723993/1‑76 (MQ=255) cTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc > 2:5402530/1‑76 (MQ=255) tGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 2:5904122/1‑76 (MQ=255) tGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt < 2:1707047/76‑1 (MQ=255) gTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 2:2056610/76‑1 (MQ=255) gTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 2:1623268/76‑1 (MQ=255) tC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 2:2531435/76‑1 (MQ=255) c‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCACCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 1:4712531/1‑76 (MQ=255) ggAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 1:3565044/76‑1 (MQ=255) ggAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt > 1:8312337/1‑76 (MQ=255) gAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 1:636932/76‑1 (MQ=255) gAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:3191025/76‑1 (MQ=255) gAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa > 2:2877748/1‑76 (MQ=255) gAAAT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 1:9252278/76‑1 (MQ=255) aaaT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:1833133/1‑76 (MQ=255) aaT‑CAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc > 1:2313438/1‑76 (MQ=255) acAGCCACAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa < 2:7941201/75‑1 (MQ=255) cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 1:1734996/76‑1 (MQ=255) cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 2:8843313/76‑1 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc > 2:9174320/1‑76 (MQ=255) ccccAATAACAGAAAAACGCCAATCCCCACAAGATAACGCCTGACGATTGTATTAAACAGGCTTTTTACCGATgcg > 2:4606162/1‑74 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 2:3419986/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 2:5705889/1‑76 (MQ=255) aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc > 2:4293112/1‑76 (MQ=255) aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc > 1:7546087/1‑76 (MQ=255) aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc > 1:7926245/1‑76 (MQ=255) aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc < 2:2747909/76‑1 (MQ=255) tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg < 2:7628375/76‑1 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:1767116/1‑76 (MQ=255) aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg < 2:1637146/76‑1 (MQ=255) gCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc < 1:2039571/76‑1 (MQ=255) caaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:9488793/75‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 1:7998643/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 1:5102489/1‑76 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 1:7737609/76‑1 (MQ=255) cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg > 2:6020885/1‑76 (MQ=255) cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg > 1:3215050/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:5723101/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCACCGCGCCCCAgccgcc < 2:3129469/76‑1 (MQ=255) cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca > 1:2628631/1‑76 (MQ=255) cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAGGGCGCATCGCGCACCAGCCGCca < 2:9290042/76‑1 (MQ=255) cAACCCCCACCAGAAAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCACCGCCCCCCAGCCGCca < 2:8654154/76‑1 (MQ=255) | GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAAT‑CAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |