BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,252,018	A→G	V248A (GTA→GCA)	nusA ←	transcription elongation factor NusA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,252,018	0	A	G	100.0%	1076.7 / NA	250	V248A (GTA→GCA)	nusA	transcription elongation factor NusA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (122/128); total (122/128)

Only 100 of 256 total aligned reads displayed.

ATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTT  >  REL606/3251945‑3252090

atCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGcc                                                                        >  1:8018457/1‑76 (MQ=255)

 tCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCg                                                                       >  1:617920/1‑76 (MQ=255)

  cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCgg                                                                      >  1:9655830/1‑76 (MQ=255)

  cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCgg                                                                      >  1:662690/1‑76 (MQ=255)

  cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCgg                                                                      >  2:2974624/1‑76 (MQ=255)

    atccGCTCGCCACCCAGTTCATTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGAt                                                                    <  2:8794857/73‑1 (MQ=255)

    aTACGCTCTCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGAt                                                                    <  2:5526878/76‑1 (MQ=255)

    aTACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGAt                                                                    <  2:255757/76‑1 (MQ=255)

    aTACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGAt                                                                    <  1:8755777/76‑1 (MQ=255)

     tACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATc                                                                   <  1:4746605/76‑1 (MQ=255)

     tACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATc                                                                   >  2:9272810/1‑76 (MQ=255)

      ccGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCg                                                                  <  1:3168742/75‑1 (MQ=255)

      aCGCTCGCCCCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCg                                                                  <  1:5324889/76‑1 (MQ=255)

      aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCg                                                                  <  1:5492009/76‑1 (MQ=255)

      aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCg                                                                  >  2:8274787/1‑76 (MQ=255)

      aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCg                                                                  <  2:4837821/76‑1 (MQ=255)

       cGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGa                                                                 >  1:9103867/1‑76 (MQ=255)

        gCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGAt                                                                <  1:9401029/76‑1 (MQ=255)

        ccacGCCCCCCAGTTCAGTAAACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGAt                                                                <  2:2212847/73‑1 (MQ=255)

        ccTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGAt                                                                <  2:5618055/75‑1 (MQ=255)

         cccGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATa                                                               <  2:9092658/74‑1 (MQ=255)

         cTCGCCCCCCAGTCCATTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATa                                                               <  2:1131577/76‑1 (MQ=255)

         cTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATa                                                               <  2:6722025/76‑1 (MQ=255)

         cTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATa                                                               <  1:3591199/76‑1 (MQ=255)

         cTCCCCACCCAGTTCAGTAGACCCCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATa                                                               <  2:8299149/76‑1 (MQ=255)

          tCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATAc                                                              >  2:3877588/1‑76 (MQ=255)

           cGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACg                                                             >  2:934967/1‑76 (MQ=255)

           cGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACg                                                             >  1:758137/1‑76 (MQ=255)

            gCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGt                                                            >  1:2920230/1‑76 (MQ=255)

            gCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGt                                                            >  1:9572317/1‑76 (MQ=255)

              cccccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGttt                                                          <  2:3259778/74‑1 (MQ=255)

              cccccAGTCCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCCCCTGCCGGATCGATACGttt                                                          <  1:1763518/74‑1 (MQ=255)

              cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGttt                                                          <  2:10007887/76‑1 (MQ=255)

               ccccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATCCCTCCGCAAGCACCTGCCTGATCGATACGTTTa                                                         <  2:5390733/75‑1 (MQ=255)

               aCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTa                                                         >  2:2700309/1‑76 (MQ=255)

               aCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTa                                                         >  1:10073136/1‑76 (MQ=255)

               aCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTa                                                         <  2:8058665/76‑1 (MQ=255)

                cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTAt                                                        >  2:4591306/1‑76 (MQ=255)

                cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACACAAGCACCTGCCGGATCGATACGTTTAt                                                        >  2:410333/1‑76 (MQ=255)

                 ccAGTTCAGTAGACCCCGCCGGAACACGCGCGCCACGCATGCCTCCGCAAGCGCCTGCCGGATCGATACGTTTATc                                                       <  2:7866238/76‑1 (MQ=255)

                  cAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCg                                                      >  1:8040990/1‑76 (MQ=255)

                  cAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCg                                                      >  1:9894334/1‑76 (MQ=255)

                    gTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGtt                                                    <  2:8689320/76‑1 (MQ=255)

                    gTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGtt                                                    >  2:9208936/1‑76 (MQ=255)

                    gTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGtt                                                    <  2:4745371/76‑1 (MQ=255)

                        aGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGtt                                                >  1:7035561/1‑76 (MQ=255)

                         gTAGACACCGCCTGAACACGCGCGGCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGgtt                                               >  2:6032359/1‑76 (MQ=255)

                          tAGACCCCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGtttt                                              <  1:9278833/76‑1 (MQ=255)

                          tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGtttt                                              >  1:3299452/1‑76 (MQ=255)

                          tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGtttt                                              <  1:3338373/76‑1 (MQ=255)

                           aGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTc                                             >  1:1625453/1‑76 (MQ=255)

                             tcacCTCCAGAACACGCGCGCCACGCATTCCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCAc                                           <  2:5074965/75‑1 (MQ=255)

                               acCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACcg                                         <  2:4126099/76‑1 (MQ=255)

                                ccGCCTGACCACTCGCGCCACGCATACCTACCCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACcgc                                        <  1:2281887/76‑1 (MQ=255)

                                 cGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACcgcg                                       >  2:7018179/1‑76 (MQ=255)

                                  gCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGa                                      <  2:30517/76‑1 (MQ=255)

                                   ccTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGGTTTCACCGCGAt                                     >  2:1673021/1‑76 (MQ=255)

                                    cTGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtt                                    >  1:7355113/1‑76 (MQ=255)

                                     tGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt                                   <  2:4777364/76‑1 (MQ=255)

                                     tGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt                                   <  1:5124150/76‑1 (MQ=255)

                                     tGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt                                   >  1:3944445/1‑76 (MQ=255)

                                     tGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt                                   >  2:6440575/1‑76 (MQ=255)

                                     tGAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTATTCACCGCGAttt                                   >  2:7281754/1‑76 (MQ=255)

                                      gAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtttt                                  >  1:1196336/1‑76 (MQ=255)

                                      gAACACGCGCGCCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtttt                                  >  1:5774875/1‑76 (MQ=255)

                                            gcgcgcCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACg                            <  2:10063807/76‑1 (MQ=255)

                                            gcgcgaCACGCATCCCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACg                            <  2:1841952/76‑1 (MQ=255)

                                              gcgcCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACgag                          <  2:5005751/76‑1 (MQ=255)

                                               cgcCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACgaga                         <  2:3576489/76‑1 (MQ=255)

                                                gcCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGaa                        <  2:7623146/76‑1 (MQ=255)

                                                gcCACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGaa                        <  2:8902090/76‑1 (MQ=255)

                                                 ccACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGCGAAc                       >  1:3720001/1‑76 (MQ=255)

                                                 ccACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAc                       <  2:9600474/76‑1 (MQ=255)

                                                 ccACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAc                       >  1:3015655/1‑76 (MQ=255)

                                                 ccACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAc                       >  1:5102278/1‑76 (MQ=255)

                                                 ccACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAc                       >  1:5994205/1‑76 (MQ=255)

                                                  cACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAcc                      <  2:3166737/76‑1 (MQ=255)

                                                  cACGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAcc                      >  1:608486/1‑76 (MQ=255)

                                                    cGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg                    >  2:9796023/1‑76 (MQ=255)

                                                    cGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg                    >  2:5759291/1‑76 (MQ=255)

                                                    cGCATACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg                    >  1:6232843/1‑76 (MQ=255)

                                                       aTACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt                 >  2:4949643/1‑76 (MQ=255)

                                                       aTACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt                 <  1:2557553/76‑1 (MQ=255)

                                                       aTACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt                 <  2:1277599/76‑1 (MQ=255)

                                                       aTACCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt                 >  1:4040667/1‑76 (MQ=255)

                                                       aTACCTACGCAAGCACCTGCCGGATCGATACGTCTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCgtac                 >  2:6809472/1‑73 (MQ=255)

                                                         aCCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcg               <  2:944952/76‑1 (MQ=255)

                                                         aCCTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcg               >  1:4872693/1‑76 (MQ=255)

                                                          ccTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcgc              >  2:6515719/1‑76 (MQ=255)

                                                          ccTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcgc              >  2:6013692/1‑76 (MQ=255)

                                                          ccTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcgc              <  1:8421390/76‑1 (MQ=255)

                                                           cTACGCAATCACCGGCCGGATCGATACGTTTATCGTTGGTTTTCACAGCGATTTTCGCACGAGAACCCGTATagca             >  2:7418520/1‑75 (MQ=255)

                                                           cTACGCAAGCCCCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcgcg             <  1:7115898/76‑1 (MQ=255)

                                                           cTACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcgcg             >  1:2010472/1‑76 (MQ=255)

                                                            tACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGa            >  2:1820955/1‑76 (MQ=255)

                                                            tACGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGa            >  2:6617333/1‑76 (MQ=255)

                                                             aCGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAg           <  2:1960531/76‑1 (MQ=255)

                                                               gCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGcc         <  1:2181561/76‑1 (MQ=255)

                                                                     aCCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCgctgct   >  1:890292/1‑76 (MQ=255)

                                                                      ccTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCtt  >  1:4215454/1‑76 (MQ=255)

ATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTT  >  REL606/3251945‑3252090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`