| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,327,533 | G→T | R113S (CGT→AGT) | mreC ← | cell wall structural complex MreBCD transmembrane component MreC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,327,533 | 0 | G | T | 99.1% | 1312.9 / ‑3.5 | 328 | R113S (CGT→AGT) | mreC | cell wall structural complex MreBCD transmembrane component MreC |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (159/165); total (161/167) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 338 total aligned reads displayed.
ATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACT > REL606/3327460‑3327605 | aTAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCa > 2:7096192/1‑76 (MQ=255) tAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATACTGACTCAg > 2:7518628/1‑76 (MQ=255) aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGc > 1:9167530/1‑76 (MQ=255) aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGc > 2:4340793/1‑76 (MQ=255) aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGc < 1:7473729/76‑1 (MQ=255) aacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCggg < 2:2682110/76‑1 (MQ=255) ttGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGaa < 2:4736382/76‑1 (MQ=255) ttGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGaa < 2:6550654/76‑1 (MQ=255) tGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAAc < 1:7102187/76‑1 (MQ=255) aTCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAAccc < 1:3220947/76‑1 (MQ=255) aTCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAAccc > 1:3073088/1‑76 (MQ=255) tCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCca < 2:1862606/76‑1 (MQ=255) tCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCca > 1:6212559/1‑76 (MQ=255) tCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCca < 2:407015/76‑1 (MQ=255) cGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcag < 2:5125069/76‑1 (MQ=255) cGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcag > 2:8017740/1‑76 (MQ=255) cGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcag > 2:8700725/1‑76 (MQ=255) gCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCcagc > 1:6235672/1‑76 (MQ=255) cTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcagca < 1:9261838/76‑1 (MQ=255) cTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcagca > 2:3584087/1‑76 (MQ=255) cTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcagca > 1:1811269/1‑76 (MQ=255) cTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACACAGCGGGGAACCcagca > 1:4564496/1‑76 (MQ=255) tataAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcagcag > 2:10042130/1‑76 (MQ=255) tataAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcagcag > 1:2101562/1‑76 (MQ=255) aaGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTc > 1:2519273/1‑76 (MQ=255) aGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTcg < 2:3703439/76‑1 (MQ=255) aGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTcc > 2:3327571/1‑75 (MQ=255) gATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTcgcg < 1:4874774/76‑1 (MQ=255) aTCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCgcgc > 1:9780308/1‑76 (MQ=255) tCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCgcgcc > 1:667749/1‑75 (MQ=255) tCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCa < 1:10090469/76‑1 (MQ=255) tCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCa < 1:8885249/76‑1 (MQ=255) tCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCa < 1:5283074/76‑1 (MQ=255) cGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAg > 2:5334828/1‑76 (MQ=255) cGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAg < 2:2189614/76‑1 (MQ=255) tttAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGa < 1:2937597/75‑1 (MQ=255) gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGa > 1:9635207/1‑76 (MQ=255) gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCGCAGa > 1:2693295/1‑76 (MQ=255) gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCGCAGa > 2:2006429/1‑76 (MQ=255) ttAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGAc < 2:2927286/76‑1 (MQ=255) ttAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCGCAGAc > 2:4654184/1‑76 (MQ=255) tAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCACCGAcg > 2:6931260/1‑76 (MQ=255) aaCCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGAcgc < 1:5459915/76‑1 (MQ=255) aaCCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCGCCGAcgc > 2:3819790/1‑76 (MQ=255) ccGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGAcgcgc > 2:9497604/1‑76 (MQ=255) cGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGAcgcgcg < 2:418639/76‑1 (MQ=255) gTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGt > 1:8142064/1‑76 (MQ=255) gTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCGCAGACGCGCGt > 1:1626547/1‑76 (MQ=255) tGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGAcgcgcggt > 2:9146764/1‑74 (MQ=255) tGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGtt < 2:9291021/76‑1 (MQ=255) tGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGtt < 1:4098328/76‑1 (MQ=255) tGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGtt < 2:64076/76‑1 (MQ=255) gagaTAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTtct < 2:5878630/76‑1 (MQ=255) agaTAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTtctc < 1:9068370/76‑1 (MQ=255) gaTAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGGTTTcc > 2:7400990/1‑76 (MQ=255) aTAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCt < 2:7784810/76‑1 (MQ=255) aaCCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCCGACCCGCGCTCTCCCGt > 1:1053010/1‑76 (MQ=255) aaCCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGt > 1:7583547/1‑76 (MQ=255) aaCCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGt < 2:9032823/76‑1 (MQ=255) aaCCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCCGt > 1:136792/1‑76 (MQ=255) aaCCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGGTCTCCTGt > 2:3932110/1‑76 (MQ=255) ccTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCCGCCGCGCGTTCTCCCGttt > 1:8613912/1‑76 (MQ=255) ccTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGttt < 2:151151/76‑1 (MQ=255) ccTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGttt < 2:6754423/76‑1 (MQ=255) ccTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGttt < 2:8794379/76‑1 (MQ=255) cTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTg < 1:5613359/76‑1 (MQ=255) cTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTg < 1:501409/76‑1 (MQ=255) cTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGGTCTCCCGtttt > 2:9385872/1‑75 (MQ=255) tGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGt < 1:4392844/76‑1 (MQ=255) tGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGt < 2:1434400/76‑1 (MQ=255) aGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAt < 2:8192461/76‑1 (MQ=255) aGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGGTCTCCTGTTTGTAt > 2:7066415/1‑76 (MQ=255) gTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAtt < 2:790234/76‑1 (MQ=255) tCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAttt > 1:7914675/1‑75 (MQ=255) tCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTg < 1:59511/76‑1 (MQ=255) cACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGt > 1:6419855/1‑76 (MQ=255) cACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGt < 1:3525460/76‑1 (MQ=255) cACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGt < 2:3647111/76‑1 (MQ=255) aCCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTc < 2:9748353/76‑1 (MQ=255) ccATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTcc < 1:9361609/76‑1 (MQ=255) cATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCa > 1:7149800/1‑76 (MQ=255) cATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCa < 1:4008392/76‑1 (MQ=255) aTTTTCTGCTCATCCTGACTCAGCGGGGAGCCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCaa < 1:3643395/76‑1 (MQ=255) aTTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCCGACGCGCGTTCTCCTGTTTGTATTGTCCaa > 1:9907295/1‑76 (MQ=255) aTTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCaa > 2:10142365/1‑76 (MQ=255) ttttCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCCCGTTCTCCTGTTTGTATTGTCCCAg > 2:1715848/1‑76 (MQ=255) tttCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGc < 1:2037624/76‑1 (MQ=255) tttCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGc < 1:8290423/76‑1 (MQ=255) tttCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGc > 1:679905/1‑76 (MQ=255) tCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCAt < 2:303168/76‑1 (MQ=255) cggCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATc < 1:1148263/74‑1 (MQ=255) tGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATca < 2:9948403/76‑1 (MQ=255) gCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcag < 2:1358700/76‑1 (MQ=255) gCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcag < 1:8440926/76‑1 (MQ=255) aTCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcag < 1:8660155/72‑1 (MQ=255) tCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGtt < 1:375605/76‑1 (MQ=255) tGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCAc < 1:2438179/76‑1 (MQ=255) tGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGGTCTCCCGTTTTTATTGTCCCAGCATCAGCAGTTTAc > 2:2702983/1‑76 (MQ=255) gACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACt < 2:560518/76‑1 (MQ=255) gACTCAGCGGGGAACCCAGCAGCTCCCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATAAGCAGTTCACt > 2:7600241/1‑76 (MQ=255) | ATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACT > REL606/3327460‑3327605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |