Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	365,031	C→A	V249V (GTC→GTA)	sbmA →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	365,031	0	C	A	100.0%	137.4 / NA	39	V249V (GTC→GTA)	sbmA	predicted transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/20);  total (19/20)

CGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCAAACTGCCGGGGCTGGAGTTTAAAAACCAG  >  REL606/364958‑365103                                                                          |                                                                         cGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATg                                                                        >  2:339665/1‑76 (MQ=255)  gCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCGGCAATCGTATgg                                                                       >  2:792958/1‑76 (MQ=255)   ctctCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGt                                                                      <  2:1245897/76‑1 (MQ=255)   ctctCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGt                                                                      >  2:903304/1‑76 (MQ=255)     ctcCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCg                                                                    >  2:709371/1‑76 (MQ=255)     ctcCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCg                                                                    >  2:195816/1‑76 (MQ=255)     ctcCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCg                                                                    >  2:181402/1‑76 (MQ=255)      cccGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGc                                                                   <  1:322585/75‑1 (MQ=255)       ccGCGCATGTGCGGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCt                                                                  <  1:339667/76‑1 (MQ=255)       ccGCGCATGTGCCGGCGCTGCCGATTCTCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGGACGGTCGCt                                                                  >  2:1272763/1‑76 (MQ=255)       ccGCGCATGTGCCGGAGCTGTCGATTATCGGGCACATTCCGTATGGTCTGGTGCTTGCCGCAATCGTATGGTCGCt                                                                  >  2:127790/1‑76 (MQ=255)       ccGCGCATGTGCCGGAGCTGCCGTTTATCGGGCGCAGTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCt                                                                  >  2:1272769/1‑76 (MQ=255)       ccGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCt                                                                  >  2:913662/1‑76 (MQ=255)       ccGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCt                                                                  >  2:703336/1‑76 (MQ=255)        cgcgCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTg                                                                 >  2:1257461/1‑76 (MQ=255)         gcgcATGTGCCGGAGCTGCCGACTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGa                                                                >  1:629594/1‑76 (MQ=255)          cgcATGTGCCGGAGCTGCCGATTATCGGGCACATCCCNTATGGTCTGGTGATTGCCGCAATCGTATGGCCGCTgct                                                               >  1:1439410/1‑74 (MQ=255)            cATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATgg                                                             <  2:1067514/76‑1 (MQ=255)               gtgCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGa                                                          <  2:764341/76‑1 (MQ=255)                   cGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCgn                                                      <  1:429506/76‑2 (MQ=255)                    ggAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGCACGGa                                                     >  2:569651/1‑76 (MQ=255)                         tGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCACTGATGGGGACCGGATTGCt                                                <  2:121649/76‑1 (MQ=255)                          gCCNATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTg                                               >  1:1332105/1‑76 (MQ=255)                           ccGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTgg                                              >  1:649106/1‑76 (MQ=255)                               ttATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGt                                          >  2:720140/1‑76 (MQ=255)                                tATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTg                                         <  2:722438/76‑1 (MQ=255)                                     ggCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAgg                                    <  2:424953/76‑1 (MQ=255)                                        acaTTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGAt                                 <  2:373758/76‑1 (MQ=255)                                         caTTCTGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGNATc                                <  1:1330306/76‑1 (MQ=255)                                         caTTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATc                                <  1:114731/76‑1 (MQ=255)                                         caTTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATc                                <  2:798839/76‑1 (MQ=255)                                          aTTCCGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGTGGACCGGATTGCTGGCAGTGGTAGGGATCa                               >  2:91814/1‑76 (MQ=255)                                            tCCGTATGGTCTGGTGATTGCCGCAATCGTAGTGTCGCTGATGGGGACCGGATTGCTGGCAGTGTTAGGGATCaaa                             <  1:82280/76‑1 (MQ=255)                                             ccGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCAAAc                            <  1:966816/76‑1 (MQ=255)                                              cGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGNATCAAACt                           <  1:1420649/76‑1 (MQ=255)                                              cGTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCANACt                           <  1:1334091/76‑1 (MQ=255)                                               gTATGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCAAACTg                          >  2:966742/1‑76 (MQ=255)                                                 aTGGTCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCAAACTGcc                        >  1:47285/1‑76 (MQ=255)                                                     tCTGGTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCAAACTGCCgggg                    <  2:861331/76‑1 (MQ=255)                                                        ggTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCAAACTGTNGGGGCTg                 <  2:1187685/76‑1 (MQ=255)                                                         gTGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCAAACTGCCGGGGCTgg                <  1:946984/76‑1 (MQ=255)                                                          tGATTGCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCAAACTGCCGGGGCTGGa               >  1:1001210/1‑76 (MQ=255)                                                               gCCGCAATCGTATGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCAAACTGCCGGGGCTGGAGTTTa          <  1:1083365/76‑1 (MQ=255)                                                                       cGTATGGTCGCTGATGGGGAACGGATTGCTGGCAGTGGTAGGGATCAAACTGGCGGGGATGGAGTTTAAAAACCAg  <  1:1474405/76‑1 (MQ=255)                                                                          |                                                                         CGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATCAAACTGCCGGGGCTGGAGTTTAAAAACCAG  >  REL606/364958‑365103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑