Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	474,884	A→C	L141* (TTA→TGA)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	474,884	0	A	C	100.0%	147.0 / NA	35	L141* (TTA→TGA)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/14);  total (21/14)

AGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGAT  >  REL606/474812‑474954                                                                         |                                                                       aGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCaattcatt                                                                     <  1:149119/76‑1 (MQ=255)      cAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGt                                                                >  2:839880/1‑76 (MQ=255)       aGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGtt                                                               >  1:545978/1‑76 (MQ=255)         aaCCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTTCTGttct                                                             >  1:58630/1‑76 (MQ=255)         aaCCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGttct                                                             <  1:932994/76‑1 (MQ=255)         aaCCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGttct                                                             <  1:748372/76‑1 (MQ=255)          aCCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGttctt                                                            >  1:1379748/1‑76 (MQ=255)           ccATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGttcttc                                                           <  2:745626/76‑1 (MQ=255)            cATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCa                                                          <  1:879971/76‑1 (MQ=255)            cATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCa                                                          <  1:829376/76‑1 (MQ=255)                 ccAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCTTTCTTCAAGTgc                                                     <  2:443326/76‑1 (MQ=255)                  cAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACAGTCAATTCATTGCCGTTCTTCAAGTgcc                                                    >  1:236709/1‑75 (MQ=255)                   aGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTgcgc                                                   >  2:404000/1‑76 (MQ=255)                     gTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTgcgcgc                                                 <  2:188155/76‑1 (MQ=255)                     gTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTgcgcgc                                                 <  2:807795/76‑1 (MQ=255)                      tCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCa                                                >  1:1183500/1‑76 (MQ=255)                                 caaccaacCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACg                                     >  1:496942/1‑76 (MQ=255)                                 caaccaacCGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACg                                     >  2:914945/1‑76 (MQ=255)                                      aaccGATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACt                                <  1:508489/76‑1 (MQ=255)                                         cGATGGCGATTTGCCCACGCTGACTGGTAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGc                             <  2:956039/76‑1 (MQ=255)                                          gATNGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCg                            >  1:1327348/1‑76 (MQ=255)                                          gATGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCg                            >  1:994989/1‑76 (MQ=255)                                          gATGGCGATTTGCCCACACTTACTGTCAATTCATTGCCGTTCTTCAAGTGCGCACAGTTACGCCACGGTACTAGCg                            >  1:75793/1‑76 (MQ=255)                                           aTGGCGATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCgg                           <  2:991552/76‑1 (MQ=255)                                            tGGCGATTTGCCCACGCTGACTGTCAATTCANAGCCATTATACAAGTGCGCGCAGTAACACCAGNGTACTGGCggg                          >  1:180681/1‑75 (MQ=255)                                                gATTTGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGaa                      <  1:358673/76‑1 (MQ=255)                                                    tGCCCACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGAc                  >  1:1046812/1‑76 (MQ=255)                                                        cACGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATaa              <  2:1429008/76‑1 (MQ=255)                                                         aCGCTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATaaa             <  2:1314608/76‑1 (MQ=255)                                                          cGCTGACTCTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACATCACGGTACTCGCGGTGGGAAGACATAAAc            >  2:23587/1‑76 (MQ=255)                                                            cTGACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCg          >  1:1147746/1‑76 (MQ=255)                                                              gACTGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGGAAGACATAAACCGaa        >  2:767133/1‑76 (MQ=255)                                                              gACTGTCAATTCATTGCCGTTCTTCAAGTGAGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGaa        >  2:1273921/1‑76 (MQ=255)                                                                 tGTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAacac     >  1:656443/1‑76 (MQ=255)                                                                  gTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGGAAGACATAAACCGAacaag    >  2:543015/1‑74 (MQ=255)                                                                  gTCAATTCATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACg    >  2:955645/1‑76 (MQ=255)                                                                    cAATTCATTGCCGTTCTTAAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGAt  >  1:208450/1‑76 (MQ=255)                                                                         |                                                                       AGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCACGCTGACTGTCAATTAATTGCCGTTCTTCAAGTGCGCGCAGTAACACCACGGTACTGGCGGTGGAAAGACATAAACCGAACACGAT  >  REL606/474812‑474954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑